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CD3D Mouse mAb - Primary antibody, specific to CD3D, Mouse IgG2b

  • Azide Free
  • Carrier Free
  • ExactAb™
  • Validated
  • 高性能
  • Lot by Lot
Features and benefits
  • Short Overview:

    mAb (C6); Mouse anti Human CD3D Antibody; WB, IHC; Unconjugated

  • Species reactivity(Reacts with): Human,Pig
  • Isotype: Mouse IgG2b
    Application:
  • IHC
  • WB
Item Number
Ab094722
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab094722-10μl
10μl
In stock
$86.90
Ab094722-20μl
20μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$89.90
Ab094722-50μl
50μl
In stock
$179.90
Ab094722-100μl
100μl
In stock
$249.90
Ab094722-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$1,399.90

mAb (C6); Mouse anti Human CD3D Antibody; WB, IHC; Unconjugated

View related series
Accession#:P04234 CD3 CD3D Gene ID:915

Basic Description

Product NameCD3D Mouse mAb - Primary antibody, specific to CD3D, Mouse IgG2b
SynonymsCD3 antigen delta subunit antibody|CD3 delta antibody|CD3-delta antibody|CD3d antibody|CD3d antigen delta polypeptide (TiT3 complex) antibody|CD3d molecule antibody|CD3d molecule delta (CD3-TCR complex) antibody|CD3D_HUMAN antibody|IMD19 antibody|OKT3 del
Specifications & PurityExactAb™, Validated, Carrier Free, Azide Free, High performance, Lot by Lot
Host speciesMouse
SpecificityCD3D
ImmunogenRecombinant CD3D expressed in E.coli (AA 18-113).
Positive ControlWB: Jurkat and MOLT-4 cell lysates. IHC: Human small intestine and spleen tissues.
ConjugationUnconjugated
GradeAzide Free, Carrier Free, ExactAb™, Validated, 高性能
Product Description

Mouse anti Human CD3D Antibody, Monoclonal (C6), could be used for WB, IHC and so on.

Application

WB: 0.01-2 μg/mL

IHC: 5-20 μg/mL

Protein Function

The CD3 complex mediates signal transduction.

Product Properties

IsotypeMouse IgG2b
Light Chain Typekappa
SDS-PAGE150 kDa
Purification MethodProtein A purified + Protein G purified
FormLiquid
ConcentrationLot by Lot
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

CD3D Mouse mAb (Ab094722) - Western Blot
All lanes: CD3D Mouse mAb (Ab094722) at 1/500 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution

Predicted band size: 19 kDa
Observed band size: 23 kDa
Exposure time: 20.0 s

CD3D Mouse mAb (Ab094722) - IHC
DAB staining on IHC
Sample: Human Small intestine Tissue
Primary Ab: 10 µg/mL CD3D Mouse mAb (Ab094722)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

CD3D Mouse mAb (Ab094722) - IHC
DAB staining on IHC
Sample: Human Spleen Tissue
Primary Ab: 10 µg/mL CD3D Mouse mAb (Ab094722)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

Associated Targets

CD3D Tclin T-cell surface glycoprotein CD3 delta chain 0 Activities

Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Application

ApplicationDilution info
IHC

5-20 µg/mL

WB

0.01-2 µg/mL

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section

3 results found

Lot NumberCertificate TypeDateItem
ZJ24F0404441Certificate of AnalysisApr 11, 2024 Ab094722
ZJ24F0404440Certificate of AnalysisApr 11, 2024 Ab094722
ZJ24F0404439Certificate of AnalysisApr 11, 2024 Ab094722

Related Documents

References

1. de Saint Basile G, Geissmann F, Flori E, Uring-Lambert B, Soudais C, Cavazzana-Calvo M, Durandy A, Jabado N, Fischer A, Le Deist F.  (2004)  Severe combined immunodeficiency caused by deficiency in either the delta or the epsilon subunit of CD3..  J Clin Invest,  114  (10): (1512-7).  [PMID:15546002]
2. Todd D Taylor,Hideki Noguchi,Yasushi Totoki,Atsushi Toyoda,Yoko Kuroki,Ken Dewar,Christine Lloyd,Takehiko Itoh,Tadayuki Takeda,Dae-Won Kim,Xinwei She,Karen F Barlow,Toby Bloom,Elspeth Bruford,Jean L Chang,Christina A Cuomo,Evan Eichler,Michael G FitzGerald,David B Jaffe,Kurt LaButti,Robert Nicol,Hong-Seog Park,Christopher Seaman,Carrie Sougnez,Xiaoping Yang,Andrew R Zimmer,Michael C Zody,Bruce W Birren,Chad Nusbaum,Asao Fujiyama,Masahira Hattori,Jane Rogers,Eric S Lander,Yoshiyuki Sakaki.  (2006-03-24)  Human chromosome 11 DNA sequence and analysis including novel gene identification..  Nature,  440  ((7083)): (497-500).  [PMID:16554811]
3. Phillips, J H JH and 5 more authors..  (1992)  Ontogeny of human natural killer (NK) cells: fetal NK cells mediate cytolytic function and express cytoplasmic CD3 epsilon,delta proteins..  The Journal of experimental medicine,    (1):   [PMID:1372642]
4. Barber, E K EK, Dasgupta, J D JD, Schlossman, S F SF, Trevillyan, J M JM and Rudd, C E CE..  (1989)  The CD4 and CD8 antigens are coupled to a protein-tyrosine kinase (p56lck) that phosphorylates the CD3 complex..  Proceedings of the National Academy of Sciences of the United States of America,      [PMID:2470098]
5. Alexander, D D and 5 more authors..  (1989)  Dephosphorylation of the human T lymphocyte CD3 antigen..  European journal of biochemistry,    (15):   [PMID:2540970]
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7. Tunnacliffe, A A, Sims, J E JE and Rabbitts, T H TH..  (1986)  T3 delta pre-mRNA is transcribed from a non-TATA promoter and is alternatively spliced in human T cells..  The EMBO journal,      [PMID:3488209]
8. van den Elsen, P P and 6 more authors..  (1984)  Isolation of cDNA clones encoding the 20K T3 glycoprotein of human T-cell receptor complex..  Nature,      [PMID:6095101]
9. Wilde, M I MI and Goa, K L KL..  (1996)  Muromonab CD3: a reappraisal of its pharmacology and use as prophylaxis of solid organ transplant rejection..  Drugs,      [PMID:8861551]
10. Doucey, Marie-Agnès MA and 7 more authors..  (2003)  CD3 delta establishes a functional link between the T cell receptor and CD8..  The Journal of biological chemistry,    (31):   [PMID:12215456]
11. Salomon, Arthur R AR and 10 more authors..  (2003)  Profiling of tyrosine phosphorylation pathways in human cells using mass spectrometry..  Proceedings of the National Academy of Sciences of the United States of America,    (21):   [PMID:12522270]
12. Dadi, Harjit K HK, Simon, Amos J AJ and Roifman, Chaim M CM..  (2003)  Effect of CD3delta deficiency on maturation of alpha/beta and gamma/delta T-cell lineages in severe combined immunodeficiency..  The New England journal of medicine,    (6):   [PMID:14602880]
13. Jin, Pei P and 7 more authors..  (2004)  PCR isolation and cloning of novel splice variant mRNAs from known drug target genes..  Genomics,      [PMID:15028279]
14. Brill, Laurence M LM and 5 more authors..  (2004)  Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity chromatography and tandem mass spectrometry..  Analytical chemistry,    (15):   [PMID:15144186]
15. Arnett, Kelly L KL, Harrison, Stephen C SC and Wiley, Don C DC..  (2004)  Crystal structure of a human CD3-epsilon/delta dimer in complex with a UCHT1 single-chain antibody fragment..  Proceedings of the National Academy of Sciences of the United States of America,    (16):   [PMID:15534202]
16. Mayya, Viveka V and 7 more authors..  (2009)  Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions..  Science signaling,    (18):   [PMID:19690332]
17. Yu, Grace P and 13 more authors..  (2011)  Genotype, phenotype, and outcomes of nine patients with T-B+NK+ SCID..  Pediatric transplantation,      [PMID:21883749]
18. Zugmaier, Gerhard G, Klinger, Matthias M, Schmidt, Margit M and Subklewe, Marion M..  (2015)  Clinical overview of anti-CD19 BiTE(®) and ex vivo data from anti-CD33 BiTE(®) as examples for retargeting T cells in hematologic malignancies..  Molecular immunology,      [PMID:25883042]

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