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CD43 Mouse mAb - Primary antibody, specific to CD43, Mouse IgG1

  • Carrier Free
  • ExactAb™
  • Validated
  • Lot by Lot
Features and benefits
  • Short Overview:

    mAb (HI161); Mouse anti Human CD43 Antibody; Flow; Unconjugated

  • Species reactivity(Reacts with): Human
  • Isotype: Mouse IgG1
    Application:
  • Flow Cytometry
Item Number
Ab094843
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab094843-10μg
10μg
In stock
$29.90
Ab094843-50μg
50μg
In stock
$69.90
Ab094843-100μg
100μg
In stock
$109.90
Ab094843-1mg
1mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$899.90

mAb (HI161); Mouse anti Human CD43 Antibody; Flow; Unconjugated

View related series
Accession#:P16150 CD43 Gene ID:6693 SPN

Basic Description

Product NameCD43 Mouse mAb - Primary antibody, specific to CD43, Mouse IgG1
SynonymsGalactoglycoprotein | GALGP | GPL115 | Leukocyte sialoglycoprotein | Sialophorin | LEU-22 | CD43 antigen | CD43 | CD43) | Galactoglycoprotein | GALGP | GPL115 | Leukocyte sialoglycoprotein | Leukosialin | LSN | Ly-48 | sialophorin (gpL115, leukosialin, CD
Specifications & PurityExactAb™, Validated, Carrier Free, Lot by Lot
Host speciesMouse
SpecificityCD43
ImmunogenHuman T lymphocytes
Positive ControlFlow: Human peripheral blood lymphocytes.
ConjugationUnconjugated
GradeCarrier Free, ExactAb™, Validated
Product Description

Mouse anti Human CD43 Antibody, Monoclonal (HI161), could be used for Flow and so on.
Application:
Flow:<0.5 µg/100w cells
Protein Function
One of the major glycoproteins of thymocytes and T lymphocytes. Plays a role in the physicochemical properties of the T-cell surface and in lectin binding. Presents carbohydrate ligands to selectins. Has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. Is a counter receptor for SN/Siglec-1 (By similarity). During T-cell activation is actively removed from the T-cell-APC (antigen-presenting cell) contact site thus suggesting a negative regulatory role in adaptive immune response.

Product Properties

IsotypeMouse IgG1
Light Chain Typekappa
SDS-PAGE150 kDa
Purification MethodProtein A purified
FormLiquid
ConcentrationLot by Lot
Storage TempStore at 2-8°C
Shipped InWet ice
Stability And StorageShipped at 4°C. Store at 4°C long term (36 months). Upon delivery aliquot.

Images

CD43 Mouse mAb (Ab094843) - Flow Cytometry
Flow Cytometry analysis of K562 cells labelling CD43 (red) with CD43 Mouse mAb (Ab094843) at 0.5 μg/mL for 1 hour at 4°C. Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

CD43 Mouse mAb (Ab094843) - Flow Cytometry
Flow Cytometry analysis of U937 cells labelling CD43 (red) with CD43 Mouse mAb (Ab094843) at 0.05 μg/mL for 1 hour at 4°C. Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

CD43 Mouse mAb (Ab094843) - Flow Cytometry
Flow Cytometry analysis of Ramos cells labelling CD43 (red) with CD43 Mouse mAb (Ab094843) at 0.5 μg/mL for 1 hour at 4°C. Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Associated Targets

SPN Tbio Leukosialin 0 Activities

Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Application

ApplicationDilution info
Flow

< 0.5µg/100w cells

Certificates

Certificate of Analysis(COA)

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3 results found

Lot NumberCertificate TypeDateItem
ZJ24F0505961Certificate of AnalysisMay 22, 2024 Ab094843
ZJ24F0505962Certificate of AnalysisMay 22, 2024 Ab094843
ZJ24F0505963Certificate of AnalysisMay 22, 2024 Ab094843

Related Documents

References

1. Schmid, K K and 9 more authors..  (1992)  Amino acid sequence of human plasma galactoglycoprotein: identity with the extracellular region of CD43 (sialophorin)..  Proceedings of the National Academy of Sciences of the United States of America,    (15):   [PMID:1731338]
2. Kudo, S S and Fukuda, M M..  (1991)  A short, novel promoter sequence confers the expression of human leukosialin, a major sialoglycoprotein on leukocytes..  The Journal of biological chemistry,    (5):   [PMID:1827122]
3. Shelley, C S CS, Remold-O'Donnell, E E, Rosen, F S FS and Whitehead, A S AS..  (1990)  Structure of the human sialophorin (CD43) gene. Identification of features atypical of genes encoding integral membrane proteins..  The Biochemical journal,    (15):   [PMID:2241892]
4. Pallant, A A and 6 more authors..  (1989)  Characterization of cDNAs encoding human leukosialin and localization of the leukosialin gene to chromosome 16..  Proceedings of the National Academy of Sciences of the United States of America,      [PMID:2521952]
5. Shelley, C S CS and 6 more authors..  (1989)  Molecular characterization of sialophorin (CD43), the lymphocyte surface sialoglycoprotein defective in Wiskott-Aldrich syndrome..  Proceedings of the National Academy of Sciences of the United States of America,      [PMID:2784859]
6. Remold-O'Donnell, E E, Davis, A E AE, Kenney, D D, Bhaskar, K R KR and Rosen, F S FS..  (1986)  Purification and chemical composition of gpL115, the human lymphocyte surface sialoglycoprotein that is defective in Wiskott-Aldrich syndrome..  The Journal of biological chemistry,    (5):   [PMID:3711098]
7. Andersson, Christian X CX and 6 more authors..  (2004)  CD43 has a functional NLS, interacts with beta-catenin, and affects gene expression..  Biochemical and biophysical research communications,    (26):   [PMID:15003504]
8. Ramírez-Pliego, Oscar O and 7 more authors..  (2007)  CD43 signals induce Type One lineage commitment of human CD4+ T cells..  BMC immunology,    (23):   [PMID:18036228]
9. Zahedi, René P RP and 8 more authors..  (2008)  Phosphoproteome of resting human platelets..  Journal of proteome research,      [PMID:18088087]
10. Mambole, Agnès A and 7 more authors..  (2008)  The cleavage of neutrophil leukosialin (CD43) by cathepsin G releases its extracellular domain and triggers its intramembrane proteolysis by presenilin/gamma-secretase..  The Journal of biological chemistry,    (29):   [PMID:18586676]
11. Mayya, Viveka V and 7 more authors..  (2009)  Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions..  Science signaling,    (18):   [PMID:19690332]
12. Zhou, Houjiang H and 6 more authors..  (2013)  Toward a comprehensive characterization of a human cancer cell phosphoproteome..  Journal of proteome research,    (4):   [PMID:23186163]

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