Mouse anti Human CD43 Antibody, Monoclonal (HI161), could be used for Flow and so on. Application: Flow:<0.5 µg/100w cells Protein Function One of the major glycoproteins of thymocytes and T lymphocytes. Plays a role in the physicochemical properties of the T-cell surface and in lectin binding. Presents carbohydrate ligands to selectins. Has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. Is a counter receptor for SN/Siglec-1 (By similarity). During T-cell activation is actively removed from the T-cell-APC (antigen-presenting cell) contact site thus suggesting a negative regulatory role in adaptive immune response.
Product Properties
Isotype
Mouse IgG1
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Form
Liquid
Concentration
Lot by Lot
Storage Temp
Store at 2-8°C
Shipped In
Wet ice
Stability And Storage
Shipped at 4°C. Store at 4°C long term (36 months). Upon delivery aliquot.
Images
CD43 Mouse mAb (Ab094843) - Flow Cytometry Flow Cytometry analysis of K562 cells labelling CD43 (red) with CD43 Mouse mAb (Ab094843) at 0.5 μg/mL for 1 hour at 4°C. Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.
CD43 Mouse mAb (Ab094843) - Flow Cytometry Flow Cytometry analysis of U937 cells labelling CD43 (red) with CD43 Mouse mAb (Ab094843) at 0.05 μg/mL for 1 hour at 4°C. Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.
CD43 Mouse mAb (Ab094843) - Flow Cytometry Flow Cytometry analysis of Ramos cells labelling CD43 (red) with CD43 Mouse mAb (Ab094843) at 0.5 μg/mL for 1 hour at 4°C. Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.
1.Schmid, K K and 9 more authors.. (1992) Amino acid sequence of human plasma galactoglycoprotein: identity with the extracellular region of CD43 (sialophorin).. Proceedings of the National Academy of Sciences of the United States of America, (15):[PMID:1731338]
2.Kudo, S S and Fukuda, M M.. (1991) A short, novel promoter sequence confers the expression of human leukosialin, a major sialoglycoprotein on leukocytes.. The Journal of biological chemistry, (5):[PMID:1827122]
3.Shelley, C S CS, Remold-O'Donnell, E E, Rosen, F S FS and Whitehead, A S AS.. (1990) Structure of the human sialophorin (CD43) gene. Identification of features atypical of genes encoding integral membrane proteins.. The Biochemical journal, (15):[PMID:2241892]
4.Pallant, A A and 6 more authors.. (1989) Characterization of cDNAs encoding human leukosialin and localization of the leukosialin gene to chromosome 16.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:2521952]
5.Shelley, C S CS and 6 more authors.. (1989) Molecular characterization of sialophorin (CD43), the lymphocyte surface sialoglycoprotein defective in Wiskott-Aldrich syndrome.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:2784859]
6.Remold-O'Donnell, E E, Davis, A E AE, Kenney, D D, Bhaskar, K R KR and Rosen, F S FS.. (1986) Purification and chemical composition of gpL115, the human lymphocyte surface sialoglycoprotein that is defective in Wiskott-Aldrich syndrome.. The Journal of biological chemistry, (5):[PMID:3711098]
7.Andersson, Christian X CX and 6 more authors.. (2004) CD43 has a functional NLS, interacts with beta-catenin, and affects gene expression.. Biochemical and biophysical research communications, (26):[PMID:15003504]
8.Ramírez-Pliego, Oscar O and 7 more authors.. (2007) CD43 signals induce Type One lineage commitment of human CD4+ T cells.. BMC immunology, (23):[PMID:18036228]
9.Zahedi, René P RP and 8 more authors.. (2008) Phosphoproteome of resting human platelets.. Journal of proteome research, [PMID:18088087]
10.Mambole, Agnès A and 7 more authors.. (2008) The cleavage of neutrophil leukosialin (CD43) by cathepsin G releases its extracellular domain and triggers its intramembrane proteolysis by presenilin/gamma-secretase.. The Journal of biological chemistry, (29):[PMID:18586676]
11.Mayya, Viveka V and 7 more authors.. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.. Science signaling, (18):[PMID:19690332]
12.Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4):[PMID:23186163]
Solution Calculators
Molarity Calculator
Determine the necessary mass, volume, or concentration for preparing a solution.
Dilution Calculator
Determine the dilution needed to prepare a stock solution.