mAb (2D1); Mouse anti Human CD45 Antibody; Flow; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab094898-10μg | 10μg | In stock | $26.90 | |
| Ab094898-50μg | 50μg | In stock | $69.90 | |
| Ab094898-100μg | 100μg | In stock | $99.90 | |
| Ab094898-1mg | 1mg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $899.90 |
mAb (2D1); Mouse anti Human CD45 Antibody; Flow; Unconjugated
| Product Name | CD45 Mouse mAb - Primary antibody, specific to CD45, Mouse IgG1 |
|---|---|
| Synonyms | B220|CD_antigen: CD45|CD45 antigen|CD45|CD45R|EC 3.1.3.48|EC:3.1.3.48|GP180|LCA|L-CA|Leukocyte common antigen|LY5|protein tyrosine phosphatase, receptor type, c polypeptide|PTPRC|receptor-type tyrosine-protein phosphatase C|T200 Glycoprotein|T200 leukocyt |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, Azide Free, Lot by Lot |
| Host species | Mouse |
| Specificity | CD45 |
| Immunogen | Human PBMC |
| Conjugation | Unconjugated |
| Grade | Azide Free, Carrier Free, ExactAb™, Validated |
| Product Description | Mouse anti Human CD45 Antibody, Monoclonal (2D1), could be used for Flow and so on. Application Flow: 1.0 μg/100w cells Protein Function Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. |
| Isotype | Mouse IgG1 |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein G purified |
| Form | Liquid |
| Concentration | Lot by Lot |
| Storage Temp | Store at 2-8°C |
| Shipped In | Wet ice |
| Stability And Storage | Shipped at 4°C. Store at 4°C long term (12 months). Upon delivery aliquot. |
CD45 Mouse mAb (Ab094898) - Flow Cytometry
Flow Cytometry analysis of Jurkat cells labelling CD45 (red) with CD45 Mouse mAb (Ab094898) at 0.5 μg/mL for 1 hour at 4°C. Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.
CD45 Mouse mAb (Ab094898) - Flow Cytometry
Flow Cytometry analysis of THP-1 cells labelling CD45 (red) with CD45 Mouse mAb (Ab094898) at 0.5 μg/mL for 1 hour at 4°C. Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.
CD45 Mouse mAb (Ab094898) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD45 with CD45 Mouse mAb (Ab094898) conjugated with biotin (right panel) at 2 μg/mL for 1 hour at 4°C, compared with mouse IgG (Ab170221) conjugated with biotin - Isotype Control (left panel) and detected with SA-PE (rp156250).
CD45 Mouse mAb (Ab094898) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD45 (red) with CD45 Mouse mAb (Ab094898) conjugated with biotin at 2 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156250). Blue - Isotype control, mouse IgG (Ab170221) conjugated with biotin. Black - Unlabelled control, cells without incubation with primary antibody.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| Flow | 1μg/100w cells |
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To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0404666 | Certificate of Analysis | Apr 19, 2024 | Ab094898 |
| ZJ24F0404665 | Certificate of Analysis | Apr 19, 2024 | Ab094898 |
| ZJ24F0404664 | Certificate of Analysis | Apr 19, 2024 | Ab094898 |
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