CD45 Rat mAb - Primary antibody, specific to CD45, Rat IgG2b

Features and benefits
  • Short Overview:

    mAb (I3/2.3); Rat anti Mouse CD45 Antibody; Flow; Unconjugated

  • Species reactivity(Reacts with): Mouse
  • Isotype: Rat IgG2b
    Application:
  • Flow Cytometry
Item Number
Ab094914
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab094914-10μg
10μg
In stock
$16.90
Ab094914-50μg
50μg
In stock
$49.90
Ab094914-100μg
100μg
In stock
$79.90
Ab094914-1mg
1mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$519.90

mAb (I3/2.3); Rat anti Mouse CD45 Antibody; Flow; Unconjugated

Basic Description

Product NameCD45 Rat mAb - Primary antibody, specific to CD45, Rat IgG2b
SynonymsB220; CD_antigen: CD45; CD45 antigen; CD45; CD45R; EC 3.1.3.48; EC:3.1.3.48; GP180; LCA; L-CA; Leukocyte common antigen; LY5; protein tyrosine phosphatase, receptor type, c polypeptide; PTPRC; receptor-type tyrosine-protein phosphatase C; T200 Glycoprotei
Specifications & PurityExactAb™, Validated, Carrier Free, Azide Free, Lot by Lot
Host speciesRat
SpecificityCD45
ImmunogenMouse lymphoma cell line
ConjugationUnconjugated
GradeAzide Free, Carrier Free, ExactAb™, Validated
Product Description

Rat anti Mouse CD45 Antibody, Monoclonal (I3/2.3), could be used for Flow and so on.

Application

Flow: 1.0 μg/100w cells

Protein Function

Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN.

Product Properties

IsotypeRat IgG2b
SDS-PAGE150 kDa
Purification MethodProtein G purified
FormLiquid
ConcentrationLot by Lot
Storage TempStore at 2-8°C
Shipped InWet ice
Stability And StorageShipped at 4°C. Store at 4°C long term (12 months). Upon delivery aliquot.

Images

CD45 Rat mAb (Ab094914) - Flow Cytometry
Flow Cytometry analysis of mouse splenocytes cells labelling CD45 with CD45 Rat mAb (Ab094914) conjugated with biotin (right panel) at 0.2 μg/mL for 1 hour at 4°C, compared with Rat IgG Isotype Control Antibody (Biotin) (Ab180310) - Isotype Control (left panel) and detected with SA-PE (rp156250).

CD45 Rat mAb (Ab094914) - Flow Cytometry
Flow Cytometry analysis of mouse splenocytes cells labelling CD45 (red) with CD45 Rat mAb (Ab094914) conjugated with biotin at 0.2 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156250). Blue - Isotype control, Rat IgG Isotype Control Antibody (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.

Associated Targets(Human)

PTPRC Tchem Receptor-type tyrosine-protein phosphatase C (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
PTPRC Tchem Leukocyte common antigen (2317 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID

Application

ApplicationDilution info
Flow Cytometry

1μg/100w cells

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

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3 results found

Lot NumberCertificate TypeDateItem
ZJ24F0404998Certificate of AnalysisApr 29, 2024 Ab094914
ZJ24F0404997Certificate of AnalysisApr 29, 2024 Ab094914
ZJ24F0404996Certificate of AnalysisApr 29, 2024 Ab094914

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