mAb (53-6.7); Rat anti Mouse CD8 alpha Antibody (AF647); Flow
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab190218-10μg (Trial Size) Apply for free trial size（?） Every year, as a valued customer, you have the exclusive opportunity to explore and enjoy three different trial products of your choice, absolutely free! | 10μg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $47.90 | |
| Ab190218-25μg | 25μg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $95.90 | |
| Ab190218-100μg | 100μg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $206.90 |
mAb (53-6.7); Rat anti Mouse CD8 alpha Antibody (AF647); Flow
| Product Name | CD8 alpha Rat mAb (AF647) |
|---|---|
| Specifications & Purity | ExactAb™, Validated, 0.5 mg/mL |
| Biochemical and Physiological Mechanisms | Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains. |
| Host species | Rat |
| Specificity | CD8 alpha |
| Immunogen | Mouse Spleen Cells or Thymocyte Membranes |
| Positive Control | Flow: C57BL/6 mouse splenocytes. |
| Conjugation | Alexa Fluor® 647 |
| Grade | ExactAb™, Validated |
| Product Description | Rat anti Mouse CD8 alpha Antibody, Monoclonal (53-6.7), could be used for Flow, ICC/IF, WB, in vivo CD8+ T cell depletion and so on. |
| Isotype | Rat IgG2a |
|---|---|
| Light Chain Type | kappa |
| Purification Method | Protein G purified |
| Concentration | 0.5 mg/mL |
| Storage Temp | Store at 2-8°C,Protected from light,Freeze prohibited |
| Shipped In | Wet ice |
| Stability And Storage | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
CD8 alpha Rat mAb (AF647) (Ab190218) - Flow Cytometry
Flow cytometry analysis of CD8 alpha expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). Cells were stained with CD8 alpha Rat mAb (AF647) (Ab190218) at 0.1μg/test for 1 hour at 4°C. Left panel - Rat IgG (AF647) (Ab190071).
CD8 alpha Rat mAb (AF647) (Ab190218) - Flow Cytometry
Flow cytometry analysis of CD8 alpha expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were stained with CD8 alpha Rat mAb (AF647) (Ab190218) at 0.1μg/test for 1 hour at 4°C. Blue - Rat IgG (AF647) (Ab190071). Black - Unlabelled control, cells without incubation with primary antibody.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| Flow | ≤0.3μg/test |
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