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CD8 alpha Rat mAb (Biotin) - Primary antibody, specific to CD8 alpha, Rat IgG2b

  • Azide Free
  • ExactAb™
  • Validated
  • 0.5 mg/mL
Features and benefits
  • Short Overview:

    mAb (2.43); Rat anti Mouse CD8 alpha Antibody (Biotin); Flow

  • Species reactivity(Reacts with): Mouse
  • Isotype: Rat IgG2b
    Application:
  • Flow Cytometry
Item Number
Ab181175
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab181175-10μg (Trial Size)
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10μg
In stock
$29.90
Ab181175-25μg
25μg
In stock
$49.90
Ab181175-100μg
100μg
In stock
$85.90

mAb (2.43); Rat anti Mouse CD8 alpha Antibody (Biotin); Flow

Basic Description

Product NameCD8 alpha Rat mAb (Biotin) - Primary antibody, specific to CD8 alpha, Rat IgG2b
Specifications & PurityExactAb™, Validated, Azide Free, 0.5 mg/mL
Host speciesRat
SpecificityCD8 alpha
ImmunogenMouse CTL clone L3
Positive ControlFlow: Murine splenocytes
ConjugationBiotin
GradeAzide Free, ExactAb™, Validated
Product Description

Application:

Flow: ≤0.3μg/test

For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.

Product Properties

IsotypeRat IgG2b
Light Chain TypeKappa
SDS-PAGE150 kDa
Purification MethodProtein G purified
FormLiquid
Concentration0.5 mg/mL
Storage TempStore at 2-8°C,Protected from light,Freeze prohibited
Shipped InWet ice
Stability And StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Images

CD8 alpha Rat mAb (Biotin) (Ab181175) - Flow Cytometry
Flow cytometry analysis of CD8 alpha expression by mouse splenocytes cells (right panel) compared to an isotype control (left panel). Cells were stained with CD8 alpha Rat mAb (Biotin) (Ab181175) at 0.05μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Left panel - Rat IgG Isotype Control Antibody (Biotin) (Ab180310).

CD8 alpha Rat mAb (Biotin) (Ab181175) - Flow Cytometry
Flow cytometry analysis of CD8 alpha expression by mouse splenocytes cells (red) compared to an isotype control (blue). Cells were stained with CD8 alpha Rat mAb (Biotin) (Ab181175) at 0.05μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Rat IgG Isotype Control Antibody (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.

Associated Targets

CD8A Tbio T-cell surface glycoprotein CD8 alpha chain 0 Activities

Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Application

ApplicationDilution info
Flow

≤0.3μg/test

Certificates

Certificate of Analysis(COA)

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3 results found

Lot NumberCertificate TypeDateItem
ZJ24F0505127Certificate of AnalysisMay 07, 2024 Ab181175
ZJ24F0505126Certificate of AnalysisMay 07, 2024 Ab181175
ZJ24F0505125Certificate of AnalysisMay 07, 2024 Ab181175

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