CD8 alpha Rat mAb - Primary antibody, specific to CD8 alpha, Rat IgG2a
Specifications & Purity
Validated, Azide Free, Carrier Free, ≥95%(SDS-PAGE), Lot by Lot
Host species
Rat
Specificity
CD8 alpha
Immunogen
Mouse Spleen Cells or Thymocyte Membranes
Conjugation
Unconjugated
Grade
Azide Free, Carrier Free, Validated
Product Description
Rat anti Mouse CD8 alpha Antibody, Monoclonal (53-6.7), could be used for Flow, ICC/IF, WB, in vivo CD8+ T cell depletion and so on. Application Flow: Use at an assay dependent concentration. ICC/IF: Use at an assay dependent concentration. WB: Use at an assay dependent concentration. in vivo CD8+ T cell depletion: Use at an assay dependent concentration. Protein Function Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
Product Properties
Isotype
Rat IgG2a
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein G purified
Form
Liquid
Concentration
Lot by Lot
Storage Temp
Store at 2-8°C,Freeze prohibited
Shipped In
Wet ice
Stability And Storage
Shipped at 4°C. Store at 4°C long term (12 months). Do not freeze. Upon delivery aliquot.
Images
CD8 alpha Rat mAb (Ab095344) - Flow Cytometry Two-color flow cytometric analysis of CD8 alpha expression on mouse splenocytes. C57BL/6 mouse spleen cells were stained with CD3 Rat mAb (FITC) (Ab185406) and either Rat IgG Isotype Control Antibody (Biotin) (Ab180310; Left panel) or CD8 alpha Rat mAb (Ab095344; Right panel) at 0.5 μg/mL. Two-color flow cytometric dot plots showing the correlated expression of CD3 versus CD8 alpha (or Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable mononuclear cells.
