ExactAb™, Validated, Carrier Free, Azide Free, ≥95%(SDS-PAGE), Lot by Lot
Host species
Rat
Specificity
CD8 alpha
Immunogen
CBA mouse thymocytes
Conjugation
Unconjugated
Grade
Azide Free, Carrier Free, ExactAb™, Validated
Product Description
Rat anti Mouse CD8 alpha Antibody, Monoclonal (YTS 169.4), could be used for in vivo CD8+ T cell depletion, WB, Flow and so on. Application: in vivo CD8+ T cell depletion: Use at an assay dependent concentration. WB: Use at an assay dependent concentration. Flow: Use at an assay dependent concentration. Protein Function Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
Product Properties
Isotype
Rat IgG2b
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein G purified
Form
Liquid
Concentration
Lot by Lot
Storage Temp
Store at 2-8°C,Freeze prohibited
Shipped In
Wet ice
Stability And Storage
Shipped at 4°C. Store at 4°C long term (12 months). Do not freeze. Upon delivery aliquot.
Images
CD8 alpha Rat mAb (Ab095356) - Flow Cytometry Two-color flow cytometric analysis of CD8 alpha expression on mouse splenocytes. C57BL/6 mouse spleen cells were stained with CD3 Rat mAb (FITC) (Ab185406) and either Rat IgG Isotype Control Antibody (Biotin) (Ab180310; Left panel) or CD8 alpha Rat mAb (Ab095356; Right panel) at 0.5 μg/mL. Two-color flow cytometric dot plots showing the correlated expression of CD3 versus CD8 alpha (or Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable mononuclear cells.
CD8 alpha Rat mAb (Ab095356) - Flow Cytometry Flow Cytometry analysis of mouse splenocytes cells labelling CD8 alpha with CD8 alpha Rat mAb (Ab095356) conjugated with biotin (right panel) at 0.5 μg/mL for 1 hour at 4°C, compared with Rat IgG Isotype Control Antibody (Biotin) (Ab180310) (left panel) and detected with SA-PE (rp156150).
CD8 alpha Rat mAb (Ab095356) - Flow Cytometry Flow Cytometry analysis of mouse splenocytes cells labelling CD8 alpha (red) with CD8 alpha Rat mAb (Ab095356) conjugated with biotin at 0.5 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156150). Blue - Rat IgG Isotype Control Antibody (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.
Application
Application
Dilution info
WB
Use at an assay dependent concentration.
Flow
Use at an assay dependent concentration.
in vivo CD8+ T cell depletion
Use at an assay dependent concentration.
Certificates
Certificate of Analysis(COA)
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
ZJ24F0809293