mAb (HI156); Mouse anti Human CD99 Antibody; Flow; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab095548-10μg | 10μg | In stock | $29.90 | |
| Ab095548-50μg | 50μg | In stock | $69.90 | |
| Ab095548-100μg | 100μg | In stock | $109.90 | |
| Ab095548-1mg | 1mg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $899.90 |
mAb (HI156); Mouse anti Human CD99 Antibody; Flow; Unconjugated
| Product Name | CD99 Mouse mAb - Primary antibody, specific to CD99, Mouse IgG2a |
|---|---|
| Synonyms | 12E7|E2 antigen|Protein MIC2|T-cell surface glycoprotein E2 |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, Lot by Lot |
| Host species | Mouse |
| Specificity | CD99 |
| Immunogen | Human endothelial cells |
| Positive Control | Flow: Human peripheral blood lymphocytes. |
| Conjugation | Unconjugated |
| Grade | Carrier Free, ExactAb™, Validated |
| Product Description |
Mouse anti Human CD99 Antibody, Monoclonal (HI156), could be used for Flow and so on. |
| Isotype | Mouse IgG2a |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | Lot by Lot |
| Storage Temp | Store at 2-8°C |
| Shipped In | Wet ice |
| Stability And Storage | Shipped at 4°C. Store at 4°C long term (36 months). Upon delivery aliquot. |
CD99 Mouse mAb (Ab095548) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD99 with CD99 Mouse mAb (Ab095548) conjugated with biotin (right panel) at 2.0 μg/mL for 1 hour at 4°C, compared with Mouse IgG (Biotin) (Ab170221) - Isotype Control (left panel) and detected with SA-PE (rp156150).
CD99 Mouse mAb (Ab095548) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD99 (red) with CD99 Mouse mAb (Ab095548) conjugated with biotin at 2.0 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156150). Blue - Isotype control, Mouse IgG (Biotin) (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.
CD99 Mouse mAb (Ab095548) - Flow Cytometry
Flow Cytometry analysis of A549 cells labelling CD99 (red) with CD99 Mouse mAb (Ab095548) at 5.0 μg/mL for 1 hour at 4°C. Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.
CD99 Mouse mAb (Ab095548) - Flow Cytometry
Flow Cytometry analysis of A431 cells labelling CD99 (red) with CD99 Mouse mAb (Ab095548) at 0.5 μg/mL for 1 hour at 4°C. Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.
CD99 Mouse mAb (Ab095548) - Flow Cytometry
Flow Cytometry analysis of HeLa cells labelling CD99 (red) with CD99 Mouse mAb (Ab095548) at 0.5 μg/mL for 1 hour at 4°C. Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.
CD99 Mouse mAb (Ab095548) - Flow Cytometry
Flow Cytometry analysis of K562 cells labelling CD99 (red) with CD99 Mouse mAb (Ab095548) at 0.5 μg/mL for 1 hour at 4°C. Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| Flow | < 0.5µg/100w cells |
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To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0506083 | Certificate of Analysis | May 24, 2024 | Ab095548 |
| ZJ24F0506082 | Certificate of Analysis | May 24, 2024 | Ab095548 |
| ZJ24F0506081 | Certificate of Analysis | May 24, 2024 | Ab095548 |
| 1. Goodfellow, P J PJ and 5 more authors.. (1988) Absence of methylation of a CpG-rich region at the 5' end of the MIC2 gene on the active X, the inactive X, and the Y chromosome.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:2456574] |
| 2. Gelin, C C and 6 more authors.. (1989) The E2 antigen, a 32 kd glycoprotein involved in T-cell adhesion processes, is the MIC2 gene product.. The EMBO journal, [PMID:2479542] |
| 3. Darling, S M SM and 5 more authors.. (1986) Molecular genetics of MIC2: a gene shared by the human X and Y chromosomes.. Cold Spring Harbor symposia on quantitative biology, [PMID:3472717] |
| 4. Ross, Mark T MT and 281 more authors.. (2005) The DNA sequence of the human X chromosome.. Nature, (17): [PMID:15772651] |
| 5. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163] |
| 6. Bian, Yangyang Y and 9 more authors.. (2014) An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.. Journal of proteomics, (16): [PMID:24275569] |
