Chip Substrates

Item Number

C491347

• Product Name: Chip Substrates
• Specifications & Purity: spot dark

Product Description

1、Product attribute
Shelf life: 36 months
Marker enzyme: Horseradish peroxidase (HRP)
Reaction time：short (up to 20 minutes) at 20-25°C
Lot-to-lot variation：< 10 %
Boiling point : 100℃（Calculated flash point）
Flash point:：120 °C （Calculated flash point）
pH-Value (at 20 °C): 4.5-4.9 （Experimental data）
Density (20℃) : 1.0064 g/cm³
Water solubility: easily soluble
Appearance: colourless liquid
Odour: odourless
Light sensitive
Heat sensitive

2、Requirements for storage rooms and vessels
1.Keep container tightly closed.
2.Keep cool. Protect from sunlight.
3.Keep/Store only in original container.
4.Never return spills in original containers for reuse.
5. Keep away from: Food and feedingstuffs.
6. The solution can be transported at room temperature, but temperatures exceeding 30 °C have to be avoided. Shipping should be completed within one week.
7. The solution will still work beyond the expiry date, but a lower sensitivity has to be taken into account and a precipitate may occur.
8. Contaminated or leaked out substrate solution from damaged bottles should not be used and has to be destroyed.

3、Effective Components and Principle of Function
A citrate buffer system (pH 4.5 - 5.0) contains the following effective components:
Hydrogen peroxide
3,3`,5,5`-Tetramethylbenzidine (TMB)
precipitating reagent
Peroxidase catalyses the decomposition of hydrogen peroxide, taking electrons from TMB (by
oxidation). Oxidation of TMB forms a radical cation that stabilizes by dimerisation and shows the typical blue colour. This blue cation is stabilized by forming an insoluble complex with the precipitating reagent. So at positions with horseradish peroxidase activity a blue precipitate will appear. This precipitate is very fine so that even the smallest dots can be coloured regularly.

4、Biosafety information
This mixture is not classified as hazardous in accordance with Regulation (EC) No 1272/2008；
Hazardous components：none (according to Regulation (EC) No 1907/2006 (REACH))

5、Advantage
1. Rapid precipitation
2. Strong adherence to plastic surfaces
3. Short reaction time
4. Intensive dark blue colour
5. Homogeneous staining pattern

6、Instruction for usage
For bottling consider the following instructions:
•Work in a dust free and darkened room.
• Pay attention that the solution has no contact with metal parts (leading to catalysis) of your
instruments. Closed systems of silicon tubes are favoured.
• Clean all instruments and vessels very carefully.
• Never touch parts of the instruments that are in contact with the solution with the naked hand. Wear powder free gloves.
• Close the bottles immediately to minimize the influence of light and dust.
• Use bottles that are not permeable to light, made from HDPE or PP.

7. General Instructions for the Use on Microchips
• This solution should only be used by qualified laboratory staff familiar with the basics of immunological methods.
• Note: The substrate solution is very sensitive to impurities. This is why you should never immerse pipette tips in storage bottles. Unused solution is never returned to the bottle! Don't swap the bottles for the cups!
• Substrate Solutions Test systems for instrumental measurements on glass or plastic surfaces (polycarbonate or polystyrene).
• Depending on the system, it may be sufficient to wash the microarrays after incubation with the conjugate and then cover them with the substrate solution. Arrays can be developed with a rocking motion until visible color appears (approximately 5 to 10 minutes). Measurements can be performed using wet arrays or after washing with distilled water and drying. Dry microchips can be used for recording purposes. (Note: some glues will destroy colored deposits)