Collagenase from Clostridium histolyticum

  • sterile-filtered
  • suitable for release of physiologically active rat hepatocytes, Type IV-S, 0.5-5.0 FALGPA units/mg solid,≥125 CDU/mg solid
Item Number
C754917
Grouped product items
SKUSizeAvailabilityPrice Qty
C754917-50mg
50mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$94.90

Basic Description

Specifications & Puritysterile-filtered, suitable for release of physiologically active rat hepatocytes, Type IV-S, 0.5-5.0 FALGPA units/mg solid,≥125 CDU/mg solid
Biochemical and Physiological MechanismsEffective release of cells from tissue requires the action of both collagenase enzymes and the neutral protease. Collagenase is activated by four gram atom calcium (Ca2+) per mole enzyme. The culture filtrate is thought to contain at least 7 different pro
Storage TempStore at -20°C
Shipped InIce chest + Ice pads
Gradesterile-filtered
Product Description

Clostridium histolyticum consists of two classes of collagenases, which cleaves at multiple cleavage sites within the collagen triple helix. It is more effective than mammalian collagenases. Clostridial collagenase is implicated in bacterial invasion in gas gangrene.
Application:
Collagenase from Clostridium histolyticum has been used to dissociate tissue samples such as spleens and testicular tissue.
Collagenase has been used in the preparation of arterial tissue for the study of Advanced Glycosylation End Products (AGE). The enzyme has also been used along with other proteases for the disaggregation of human tumor, mouse kidney, human brain, lung epithelium and many other tissues. It is also effective in liver and kidney perfusion studies, digestion of pancreas, and isolation of nonparenchymal hepatocytes. When this enzyme is tested for suitability for the release of hepatocytes. The collagenase is used at approximately 1 mg/mL in a total volume of 100 mL for each rat liver.

Names and Identifiers

Enzyme Commission Number3.4.24.3(BRENDA,IUBMB)
Isomeric SMILES CCCCCCCC[C@H]1CC[C@@]2([C@@]1(CC[C@]3(C2CC[C@@]4([C@@]3(CC5=C(C4)N=C6C[C@]7([C@@](CCC8[C@@]7(CC[C@]9([C@]8(CC[C@@H]9CCCCCCCC)C)C)C)(CC6=N5)C)C)C)C)C)C)C
WGK Germany 1
PubChem CID 44384985

Certificates

Certificate of Analysis(COA)

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Safety and Hazards(GHS)

Pictogram(s) GHS08
Signal Warning
Hazard Statements

H334:May cause allergy or asthma symptoms or breathing difficulties if inhaled

Precautionary Statements

P261:Avoid breathing dust/fume/gas/mist/vapors/spray.

P501:Dispose of contents/container to ...

P284:[In case of inadequate ventilation] Wear respiratory protection.

WGK Germany 1

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