Combination Of Five Chemicals For Long-term Functional Maintenance Of Primary Human Hepatocytes In Vitro

Item Number

C743075

Product Description

5C is a combination of five compounds used for long-term functional maintenance of primary human hepatocyte in vitro.Liver is an important organ in the human body, responsible for metabolism, detoxification, hematopoietic coagulation, immunity and other functions. Hepatocytes are liver parenchyma cells that account for 80-90% of the liver and are the main bearers of liver function. The isolation and in vitro culture of primary hepatocytes provide important methods and materials for simulating liver diseases and studying molecular mechanisms. However, freshly isolated primary hepatocytes can only be maintained for 10-14 days in vitro, during which their functionality rapidly declines. They subsequently transform into fibroblast-like stellate cells and eventually undergo apoptosis, which presents changes for in vitro cell culture to simulate in vivo diseases and study molecular mechanisms. Therefore, maintaining the stability of hepatocytes during long-term in vitro culture and ensuring their functionality consistent with that in vivo remains a long-standing unresolved issue and challenge in the field of liver research.5C is based on "Long-term functional maintenance of primary human hepatocytes in vitro" published by Professor Deng Hongkui's research group at Peking University in April 2019 (Xiang C, et al. Science. 2019 Apr 26;364(6438)

Precautions：

The solvent of all reagents in this product is high-purity DMSO.This product may have a certain toxic effect on the human body. Care should be taken to avoid direct contact or inhalation.This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation.

Instructions for Use：

1. Store this product immediately at -20℃ upon receipt. Before opening the tube, centrifuge at 2,000-10,000×g for a few seconds to collect the liquid at the bottom of the tube.2. For the optimal working concentration, please refer to this manual or other relevant literature, or explore through experiments for specific primary cells and experimental purposes.