Cyclic-di-GMP disodium is a STING agonist and a bacterial second messenger that coordinates different aspects of bacterial growth and behavior, including motility, virulence, biofilm formation, and cell cycle progression. Cyclic-di-GMP disodium has anti-cancer cell proliferation activity and also induces elevated CD4 receptor expression and cell cycle arrest. Cyclic-di-GMP disodium can be used in cancer research
In Vitro
Cyclic-di-GMP disodium (0.5-50 µM; 5 days) inhibits proliferation of human colon cancer cells. Cyclic-di-GMP disodium (0.5-50 µM; 5 days) specifically elevates CD4 expression in Jurkat cells. Cyclic-di-GMP disodium (0.5-50 µM; 5 days) induces cell cycle arrest at the S-phase in Jurkat cells. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Cell Proliferation AssayCell Line: H508 cells Concentration: 0.5-50 µM Incubation Time: 5 days Result: Reduced basal H508 cell proliferation by approx 15%, even inhibited acetylcholine- and EGF-induced cell proliferation. Cell Viability AssayCell Line: Jurkat cells Concentration: 50 µM Incubation Time: 24 h Result: Specifically induced of CD4 (no effect on the expression of CD8), with a 6.3-fold upregulation over control and in a dose-dependent manner. Cell Cycle AnalysisCell Line: Jurkat cells Concentration: 50 µM Incubation Time: 24 h Result: Increased the percentage of cells in S-phase by 79%, with almost complete disappearance of G2/M-phase cells which decreased by 93%.
In Vivo
Cyclic-di-GMP disodium (100 µg/per; i.v.; two sequential vaccinations 9 days apart) enhances TriVax-induced immune responses to melanoma in mice and further increased the anti-tumor effects of TriVax. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Animal Model: C57BL/6 (B6) mice (8- to 10-week-old). Dosage: 100 µg/per Administration: Intravenous injection; two sequential vaccinations 9 days apart; combine with TriVax. Result: Significantly higher numbers of antigen-specific CD8 T cells when combined with TriVax. (TriVax consisted of a mixture of 120 μg Pam-hgp100, 100 µg hgp100 or 100 µg Ova, 50 or 25 μg anti-CD40 antibody, and 25 μg Poly-IC). Enhanced the anti-tumor activity of TriVax.
