mAb (C2); Mouse anti Human Cytochrome C Antibody; WB, IHC, ICC, IF; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab098695-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $66.90 | |
| Ab098695-20μl | 20μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $99.90 | |
| Ab098695-50μl | 50μl | In stock | $189.90 | |
| Ab098695-100μl | 100μl | In stock | $299.90 | |
| Ab098695-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,199.90 |
mAb (C2); Mouse anti Human Cytochrome C Antibody; WB, IHC, ICC, IF; Unconjugated
| Product Name | Cytochrome C Mouse mAb - Primary antibody, specific to CYCS, Mouse IgG2a |
|---|---|
| Synonyms | CYC antibody; CYC_HUMAN antibody; CYCS antibody; Cytochrome c antibody; Cytochrome c somatic antibody; HCS antibody; THC4 antibody; Monoclonal Antibody to Cytochrome C; 细胞色素C(CYCS)单克隆抗体 |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, Azide Free, High performance, Lot by Lot |
| Host species | Mouse |
| Specificity | CYCS |
| Immunogen | Recombinant CYCS expressed in E.coli (AA 5-100) |
| Positive Control | WB: HEK293, Jurkat, HeLa, Hep G2, A549 and LNCaP cell lysates. IHC: Human cerebellum, cardiac muscle, small intestine, lymph node tissues. ICC/IF: Hep G2, HeLa cells. |
| Conjugation | Unconjugated |
| Grade | Azide Free, Carrier Free, ExactAb™, High performance, Validated |
| Product Description | Mouse anti Human Cytochrome C Antibody, Monoclonal (C2), could be used for WB,IHC,ICC,IF and so on. Application: WB: 0.01-2µg/mL IHC: 5-30µg/mL ICC/IF: 5-30µg/mL Protein Function Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain. Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases. |
| Isotype | Mouse IgG2a |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A + Protein G purified |
| Form | Liquid |
| Concentration | Lot by Lot |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Cytochrome C Mouse mAb (Ab098695) - Western Blot
All lanes: Cytochrome C Mouse mAb (Ab098695) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution
Predicted band size: 12 kDa
Observed band size: 16 kDa
Exposure time: 7.9 s
Cytochrome C Mouse mAb (Ab098695) - IF/ICC
FITC staining on IF
Sample: Human Hep G2 cell
Primary Ab: 20 μg/mL Cytochrome C Mouse mAb (Ab098695)
Second Ab: 5 μg/mL FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
Cytochrome C Mouse mAb (Ab098695) - IF/ICC
FITC staining on IF
Sample: Human HeLa cell
Primary Ab: 30 μg/mL Cytochrome C Mouse mAb (Ab098695)
Second Ab: 2 µg/mL FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
Cytochrome C Mouse mAb (Ab098695) - IHC
DAB staining on IHC
Sample: Human Cerebellum Tissue
Primary Ab: 30 μg/mL Cytochrome C Mouse mAb (Ab098695)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
Cytochrome C Mouse mAb (Ab098695) - IHC
DAB staining on IHC
Sample: Human Cardiac Muscle Tissue
Primary Ab: 30 μg/mL Cytochrome C Mouse mAb (Ab098695)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
Cytochrome C Mouse mAb (Ab098695) - IHC
DAB staining on IHC
Sample: Human Small intestine Tissue
Primary Ab: 30 μg/mL Cytochrome C Mouse mAb (Ab098695)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
Cytochrome C Mouse mAb (Ab098695) - IHC
DAB staining on IHC
Sample: Human Lymph node Tissue
Primary Ab: 30 μg/mL Cytochrome C Mouse mAb (Ab098695)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| IHC | 5-30 µg/mL |
| WB |
0.01-2 µg/mL
|
| ICC/IF |
5-30 µg/mL
|
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F1101824 | Certificate of Analysis | Nov 27, 2023 | Ab098695 |
| ZJ23F1101821 | Certificate of Analysis | Nov 27, 2023 | Ab098695 |
| 1. Scherer SW, Cheung J, MacDonald JR, Osborne LR, Nakabayashi K, Herbrick JA, Carson AR, Parker-Katiraee L, Skaug J, Khaja R et al.. (2003) Human chromosome 7: DNA sequence and biology.. Science, 300 (5620): (767-72). [PMID:12690205] |
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| 13. San Francisco, Brian B, Bretsnyder, Eric C EC and Kranz, Robert G RG.. (2013) Human mitochondrial holocytochrome c synthase's heme binding, maturation determinants, and complex formation with cytochrome c.. Proceedings of the National Academy of Sciences of the United States of America, (26): [PMID:23150584] |
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