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Availability

Price

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D128591-2.5KU

2.5KU

Available within 4-8 weeks(?)

$232.90

D128591-10KU

10KU

Available within 4-8 weeks(?)

$841.90

Basic Description

Synonyms	Deoxyribonucleate 5′-oligonucleotido-hydrolase;DNase I
Specifications & Purity	EnzymoPure™, ≥2,000 units/mg dry weight
Biochemical and Physiological Mechanisms	DNase I is an endonuclease that acts on phosphodiester bonds adjacent to pyrimidines to produce polynucleotides with terminal 5′-phosphates. In the presence of Mg2+, DNAse I cleaves each strand of DNA independently and the cleavage sites are random. Both
Storage Temp	Store at 2-8°C
Shipped In	Wet ice
Grade	EnzymoPure™
Note	10KU卖完停产，不再备货
Product Description	Bovine pancreatic deoxyribonuclease is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding polynucleotides with free hydroxyl group at the 3' position and phosphate group at the 5' position. The average chain length of a limit digest is a tetranucleotide. Used for the removal of DNA from protein samples. Deoxyribonuclease I from bovine pancreas has been used in a study to investigate a two-dimensional zymogram analysis of nucleases in Bacillus subtilis. Deoxyribonuclease I from bovine pancreas has also been used in a study to investigate the effects of minor and major groove-binding drugs and intercalators on the DNA association of minor groove-binding proteins RecA and deoxyribonuclease I.

Names and Identifiers

Isomeric SMILES	CN(C)C1=NC(=NC(=N1)N)C(CC2=CNC3=CC=CC=C32)NC(=O)C4=CC=CC=C4
WGK Germany	3
RTECS	RF0750000
PubChem CID	597667

Certificates

Certificate of Analysis(COA)

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2 results found

Lot Number	Certificate Type	Date	Item
H1517086	Certificate of Analysis	Mar 06, 2024	D128591
F1723048	Certificate of Analysis	Feb 06, 2023	D128591

Chemical and Physical Properties

Sensitivity	Moistrue

Safety and Hazards(GHS)

WGK Germany	3
RTECS	RF0750000

FAQs and Articles

FAQ

CRISPR/Cas9 and targeted genome editing

Categories: Technical articles

Tags:

Nuclease Cell and Molecular Biology Research Targeting efficiency Off target mutation

Experimental Guide of Sample Preparation for Flow Cytometry

Categories: Protocols

Tags:

Protein sample preparation Flow Cytometry Antibody FACS

Citations of This Product

How to Cite Aladdin Scientific Products?

1. Shaopeng Liu, Dan Peng, Shuohan He, Xuan Li, Yi Wu, Xin Liu, Yang Zhang, Peng Liu, Kaiyong Cai. (2023) Ternary low-temperature phototherapy nano-systems for the treatment of diabetic wounds. Journal of Materials Chemistry B, 12 (1): (264-274). [PMID:38088036] [10.1039/D3TB02224B]
2. Chenfei Han, Yu Xiao, Zhenjiang Liu, Daolin Du, Ming Li. (2023) Cascade amplifying aptasensor for positively correlated detecting OTA: Based on DNase I-assisted cyclic enzyme digestion and AgNPs@gel-enhanced fluorescence. FOOD CONTROL, 153 (109970). [PMID:] [10.1016/j.foodcont.2023.109970]
3. Ningning Lei, Xu Peng, Mengyue Hu, Chang Wan, Xixun Yu. (2023) Research on essential performance of oxidized chitosan-crosslinked acellular porcine aorta modified with bioactive SCPP/DOPA for esophageal scaffold with enhanced mechanical strength, biocompatibility and anti-inflammatory. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 241 (124522). [PMID:37100332] [10.1016/j.ijbiomac.2023.124522]
4. Hui-Zhen Zhang, Mei Shu, Wen-Yu Yang, Hong Pan, Men-Xuan Tang, Yuan-Yang Zhao, Chan Zhong, Guo-Ping Wu. (2023) Isolation and characterization of a novel Salmonella bacteriophage JNwz02 capable of lysing Escherichia coli O157:H7 and its antibacterial application in foods. LWT-FOOD SCIENCE AND TECHNOLOGY, 173 (114251). [PMID:] [10.1016/j.lwt.2022.114251]
5. Luan Zhaohui, Liu Shuang, Wang Wei, Xu Kaige, Ye Shaosong, Dan Ruijue, Zhang Hong, Shu Zhenzhen, Wang Tongchuan, Fan Chaoqiang, Xing Malcolm, Yang Shiming. (2022) Aligned nanofibrous collagen membranes from fish swim bladder as a tough and acid-resistant suture for pH-regulated stomach perforation and tendon rupture. Biomaterials Research, 26 (1): (1-20). [PMID:36348451] [10.1186/s40824-022-00306-1]
6. Can Cheng, Xu Peng, Linjie Xi, Yihao Luo, Yuhang Wang, Yufan Zhou, Xixun Yu. (2022) Feasibility study of oxidized naringin as a novel crosslinking agent for crosslinking decellularized porcine Achilles tendon and its potential application for anterior cruciate ligament repair. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 111 (2): (170-184). [PMID:36054309] [10.1002/jbm.a.37440]
7. Guangquan Hu, Xiaojie Ding, Feng Gao, Jiehua Li. (2022) Calcium and integrin binding protein 1 (CIB1) induces myocardial fibrosis in myocardial infarction via regulating the PI3K/Akt pathway. EXPERIMENTAL ANIMALS, [PMID:34349085] [10.1538/expanim.21-0063]
8. Jiansheng Liu, Xueqin Qing, Qin Zhang, Ningyue Yu, Mengbin Ding, Zhaohui Li, Zhen Zhao, Zhiling Zhou, Jingchao Li. (2021) Oxygen-producing proenzyme hydrogels for photodynamic-mediated metastasis-inhibiting combinational therapy. Journal of Materials Chemistry B, 9 (26): (5255-5263). [PMID:34138994] [10.1039/D1TB01009C]
9. Yu Luo, Shenyu Huang, Lie Ma. (2021) A novel detergent-based decellularization combined with carbodiimide crosslinking for improving anti-calcification of bioprosthetic heart valve. Biomedical Materials, 16 (4): (45022). [PMID:33979785] [10.1088/1748-605X/ac0088]
10. Yu Luo, Lie Ma. (2020) Bioprosthetic heart valves with reduced immunogenic residuals using vacuum-assisted decellularization treatment. Biomedical Materials, 15 (6): (65012). [PMID:33016260] [10.1088/1748-605X/abaabf]
11. Yu Luo, Dong Lou, Lie Ma, Changyou Gao. (2019) Optimizing detergent concentration and processing time to balance the decellularization efficiency and properties of bioprosthetic heart valves. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 107 (10): (2235-2243). [PMID:31125175] [10.1002/jbm.a.36732]
12. Xuancheng Zhang, Zhaoyi Fang, Eunshinae Cho, Kai Huang, Jinzhong Zhao, Jia Jiang, Xiaoqiao Huangfu. (2019) Use of a Novel, Reinforced, Low Immunogenic, Porcine Small Intestine Submucosa Patch to Repair a Supraspinatus Tendon Defect in a Rabbit Model. Biomed Research International, 2019 (9346567). [PMID:31073531] [10.1155/2019/9346567]
13. Ma Liang, Guo Ting, Pan Shuli, Zhang Yuhao. (2018) A fluorometric aptasensor for patulin based on the use of magnetized graphene oxide and DNase I-assisted target recycling amplification. MICROCHIMICA ACTA, 185 (10): (1-6). [PMID:30276550] [10.1007/s00604-018-3023-z]
14. Yuanting Xu, Chengcheng Huang, Li Li, Xixun Yu, Xu Wang, Hong Peng, Zhipeng Gu, Yaping Wang. (2013) In vitro enzymatic degradation of a biological tissue fixed by alginate dialdehyde. CARBOHYDRATE POLYMERS, 95 (148). [PMID:23618251] [10.1016/j.carbpol.2013.03.021]
15. Yuanting Xu, Li Li, Hao Wang, Xixun Yu, Zhipeng Gu, Chengcheng Huang, Hong Peng. (2013) In vitro cytocompatibility evaluation of alginate dialdehyde for biological tissue fixation. CARBOHYDRATE POLYMERS, 92 (448). [PMID:23218319] [10.1016/j.carbpol.2012.09.096]

References

1. Shaopeng Liu, Dan Peng, Shuohan He, Xuan Li, Yi Wu, Xin Liu, Yang Zhang, Peng Liu, Kaiyong Cai. (2023) Ternary low-temperature phototherapy nano-systems for the treatment of diabetic wounds. Journal of Materials Chemistry B, 12 (1): (264-274). [PMID:38088036] [10.1039/D3TB02224B]
2. Chenfei Han, Yu Xiao, Zhenjiang Liu, Daolin Du, Ming Li. (2023) Cascade amplifying aptasensor for positively correlated detecting OTA: Based on DNase I-assisted cyclic enzyme digestion and AgNPs@gel-enhanced fluorescence. FOOD CONTROL, 153 (109970). [PMID:] [10.1016/j.foodcont.2023.109970]
3. Ningning Lei, Xu Peng, Mengyue Hu, Chang Wan, Xixun Yu. (2023) Research on essential performance of oxidized chitosan-crosslinked acellular porcine aorta modified with bioactive SCPP/DOPA for esophageal scaffold with enhanced mechanical strength, biocompatibility and anti-inflammatory. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 241 (124522). [PMID:37100332] [10.1016/j.ijbiomac.2023.124522]
4. Hui-Zhen Zhang, Mei Shu, Wen-Yu Yang, Hong Pan, Men-Xuan Tang, Yuan-Yang Zhao, Chan Zhong, Guo-Ping Wu. (2023) Isolation and characterization of a novel Salmonella bacteriophage JNwz02 capable of lysing Escherichia coli O157:H7 and its antibacterial application in foods. LWT-FOOD SCIENCE AND TECHNOLOGY, 173 (114251). [PMID:] [10.1016/j.lwt.2022.114251]
5. Luan Zhaohui, Liu Shuang, Wang Wei, Xu Kaige, Ye Shaosong, Dan Ruijue, Zhang Hong, Shu Zhenzhen, Wang Tongchuan, Fan Chaoqiang, Xing Malcolm, Yang Shiming. (2022) Aligned nanofibrous collagen membranes from fish swim bladder as a tough and acid-resistant suture for pH-regulated stomach perforation and tendon rupture. Biomaterials Research, 26 (1): (1-20). [PMID:36348451] [10.1186/s40824-022-00306-1]
6. Can Cheng, Xu Peng, Linjie Xi, Yihao Luo, Yuhang Wang, Yufan Zhou, Xixun Yu. (2022) Feasibility study of oxidized naringin as a novel crosslinking agent for crosslinking decellularized porcine Achilles tendon and its potential application for anterior cruciate ligament repair. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 111 (2): (170-184). [PMID:36054309] [10.1002/jbm.a.37440]
7. Guangquan Hu, Xiaojie Ding, Feng Gao, Jiehua Li. (2022) Calcium and integrin binding protein 1 (CIB1) induces myocardial fibrosis in myocardial infarction via regulating the PI3K/Akt pathway. EXPERIMENTAL ANIMALS, [PMID:34349085] [10.1538/expanim.21-0063]
8. Jiansheng Liu, Xueqin Qing, Qin Zhang, Ningyue Yu, Mengbin Ding, Zhaohui Li, Zhen Zhao, Zhiling Zhou, Jingchao Li. (2021) Oxygen-producing proenzyme hydrogels for photodynamic-mediated metastasis-inhibiting combinational therapy. Journal of Materials Chemistry B, 9 (26): (5255-5263). [PMID:34138994] [10.1039/D1TB01009C]
9. Yu Luo, Shenyu Huang, Lie Ma. (2021) A novel detergent-based decellularization combined with carbodiimide crosslinking for improving anti-calcification of bioprosthetic heart valve. Biomedical Materials, 16 (4): (45022). [PMID:33979785] [10.1088/1748-605X/ac0088]
10. Yu Luo, Lie Ma. (2020) Bioprosthetic heart valves with reduced immunogenic residuals using vacuum-assisted decellularization treatment. Biomedical Materials, 15 (6): (65012). [PMID:33016260] [10.1088/1748-605X/abaabf]
11. Yu Luo, Dong Lou, Lie Ma, Changyou Gao. (2019) Optimizing detergent concentration and processing time to balance the decellularization efficiency and properties of bioprosthetic heart valves. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 107 (10): (2235-2243). [PMID:31125175] [10.1002/jbm.a.36732]
12. Xuancheng Zhang, Zhaoyi Fang, Eunshinae Cho, Kai Huang, Jinzhong Zhao, Jia Jiang, Xiaoqiao Huangfu. (2019) Use of a Novel, Reinforced, Low Immunogenic, Porcine Small Intestine Submucosa Patch to Repair a Supraspinatus Tendon Defect in a Rabbit Model. Biomed Research International, 2019 (9346567). [PMID:31073531] [10.1155/2019/9346567]
13. Ma Liang, Guo Ting, Pan Shuli, Zhang Yuhao. (2018) A fluorometric aptasensor for patulin based on the use of magnetized graphene oxide and DNase I-assisted target recycling amplification. MICROCHIMICA ACTA, 185 (10): (1-6). [PMID:30276550] [10.1007/s00604-018-3023-z]
14. Yuanting Xu, Chengcheng Huang, Li Li, Xixun Yu, Xu Wang, Hong Peng, Zhipeng Gu, Yaping Wang. (2013) In vitro enzymatic degradation of a biological tissue fixed by alginate dialdehyde. CARBOHYDRATE POLYMERS, 95 (148). [PMID:23618251] [10.1016/j.carbpol.2013.03.021]
15. Yuanting Xu, Li Li, Hao Wang, Xixun Yu, Zhipeng Gu, Chengcheng Huang, Hong Peng. (2013) In vitro cytocompatibility evaluation of alginate dialdehyde for biological tissue fixation. CARBOHYDRATE POLYMERS, 92 (448). [PMID:23218319] [10.1016/j.carbpol.2012.09.096]

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Deoxyribonuclease I from Bovine Pancreas(Ribonuclease&Protease Free) - ≥2,000 units/mg dry weight, high purity , CAS No.9003-98-9