DRAQ7 Fluorescent Probe

Item Number

D266297

• Storage Temp: Store at 2-8°C,Protected from light,Desiccated
• Shipped In: Wet ice

Product Description

DRAQ7 is a far-red DNA dye that can stain nuclei in dead and permeabilized cells. Because it is impermeable to living cells, it can be used to distinguish between living cells and dead cells. It is an ideal tool for studying dead or membrane-damaged cells, and it can quickly stain the dsDNA/nucleus of dead or permeabilized cells. It can be used in most cell types, eukaryotic and prokaryotic organisms: mammals, bacteria, parasites, plants, etc. It can be used with live cell dyes and used for siRNA research and other dynamic activity detection.

DRAQ7 is an ideal substitute for PI and 7-AAD because it is not excited by ultraviolet rays and has no emission overlap with PE and PE homologues. It can be combined with FITC, PE and other purple dyes for multicolor analysis without washing or RNase treatment. DRAQ7 can be detected by flow cytometry, laser scanning cytometer and confocal microscope. DRAQ7 is optimally excited at 647nm. When using flow cytometry, excitation at 488nm, 514nm and 568nm can be used. For imaging microscopy, it is recommended to use a light source of 633 or 647 nm for excitation. Due to its wide emission and excitation wavelength range, it is not recommended to combine DRAQ7 with other far-red fluorescent dyes that can be excited at 488 or 633 nm.

Instructions

①. Prepare PBS buffer without sodium azide.

②. Fix the cells: fix in 4% paraformaldehyde in PBS for 15 min at room temperature.

③. Wash the cells twice with PBS.

④. Permeabilize the cells in 0.5% Triton X-100 in PBS for 10 min at room temperature.

⑤. Wash the cells twice with PBS.

⑥. Optional: Perform immunofluorescence staining operations according to your standards.

⑦. Dilute DRAQ7 to the best concentration according to different cells, and stain at room temperature for 5-30 min (37℃ staining is faster, and shorter staining time may be required). The recommended dilution factor is between 1:15 and 1:200.

⑧. Detect far-infrared cell nuclear staining with fluorescence microscope, flow cytometer, etc.

Precautions

1. Fluorescent dyes have quenching problems, please try to avoid light to slow down quenching.

2. For your safety and health, please wear lab coats and disposable gloves.