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Daclizumab (anti-IL2RA) - Primary antibody, specific to IL2RA, >95%, high purity, Human IgG1, INHIBITOR of Interleukin-2 receptor inhibitor

Azide Free
Carrier Free
ExactAb™
Low Endotoxin
Recombinant
Validated
≥95%(SDS-PAGE&SEC)
Lot by Lot

Features and benefits

Short Overview:
Purity>95% (SDS-PAGE&SEC); Endotoxin Level<1.0EU/mg; Human IgG1; CHO; ELISA, FACS, Functional assay, Animal Model; Unconjugated
Species reactivity(Reacts with): Human,Mouse,Rat,Rhesus monkey
Isotype: Human IgG1

Application:

Animal Model
ELISA
Flow Cytometry
Functional Assay

Item Number

Ab170892

Grouped product items
SKU	Size	Availability	Price
Ab170892-100μg	100μg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$59.90
Ab170892-1mg	1mg	Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!	$297.90
Ab170892-5mg	5mg	Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!	$777.90
Ab170892-10mg	10mg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$12,288.90

Purity>95% (SDS-PAGE&SEC); Endotoxin Level<1.0EU/mg; Human IgG1; CHO; ELISA, FACS, Functional assay, Animal Model; Unconjugated

View related series

Accession#:P01589 CD25 Gene ID:3559 IL-2 Receptor Alpha IL2RA

Basic Description

Product Name	Daclizumab (anti-IL2RA) - Primary antibody, specific to IL2RA, >95%, high purity, Human IgG1
Synonyms	Interleukin 2 receptor alpha chain antibody; CD25 antibody; CD25 antigen antibody; IDDM10 antibody; IL 2 receptor alpha subunit antibody; IL-2 receptor subunit alpha antibody; IL-2-RA antibody; IL-2R subunit alpha antibody; IL2 RA antibody; IL2 Receptor a
Specifications & Purity	ExactAb™, Validated, Carrier Free, Low Endotoxin, Azide Free, Recombinant, ≥95%(SDS-PAGE&SEC), Lot by Lot
Specificity	IL2RA
Conjugation	Unconjugated
Grade	Azide Free, Carrier Free, ExactAb™, Low Endotoxin, Recombinant, Validated
Action Type	INHIBITOR
Mechanism of action	INHIBITOR of Interleukin-2 receptor inhibitor
Product Description	Daclizumab (anti-IL2RA) is a humanized, monoclonal antibody that blocks CD25 (α-subunit of the high-affinity interleukin-2 receptor (IL-2R-HA)). Daclizumab (anti-IL2RA) reversibly binds to CD25and prevents the interaction of IL-2 with the IL-2R-HA. Daclizumab (anti-IL2RA) can be used for multiple sclerosis research. Purity >95% (SDS-PAGE&SEC) Endotoxin Level < 1.0EU/mg

Product Properties

Isotype	Human IgG1
Light Chain Type	kappa
SDS-PAGE	27.1 kDa (Light Chain) & 51.4 kDa (Heavy Chain), under reducing conditions; 178.6 kDa, under non-reducing conditions.
Purification Method	Protein A purified
Purity	>95%
Form	Liquid
Concentration	Lot by Lot
Storage Temp	Store at -80°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at -80℃ for 24 months. Upon delivery aliquot. Avoid freeze/thaw cycle.
CAS	152923-56-3

Images

Daclizumab (anti-IL2RA) (Ab170892) - Flow Cytometry
Flow Cytometry analysis of PHA-stimulated (3 days) human peripheral blood mononuclear lymphocytes labelling IL2RA (red) with Daclizumab (anti-IL2RA) (Ab170892). Goat Anti-Human IgG (PE) (Ab175838) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, human IgG (Ab170213). Black - Unlabelled control, cells without incubation with primary antibody.

Daclizumab (anti-IL2RA) (Ab170892) - SEC
The purity of Daclizumab (anti-IL2RA) (Ab170892) is more than 95% verified by HPLC.

Daclizumab (anti-IL2RA) (Ab170892) - ELISA
Immobilized TARGET at 2 μg/mL can bind Daclizumab (anti-IL2RA) (Ab170892) with the EC₅₀ of 31.47 ng/mL.

Certificates

Certificate of Analysis(COA)

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2 results found

Lot Number	Certificate Type	Date	Item
ZJ23F1001292	Certificate of Analysis	Oct 13, 2023	Ab170892
ZJ23F1001293	Certificate of Analysis	Oct 13, 2023	Ab170892

References

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2. Leonard, W J WJ and 7 more authors.. (1985) Structure of the human interleukin-2 receptor gene.. Science (New York, N.Y.), (8): [PMID:2996141]
3. Ishida, N N and 7 more authors.. (1985) Molecular cloning and structure of the human interleukin 2 receptor gene.. Nucleic acids research, (11): [PMID:2999698]
4. Cross, S L SL and 5 more authors.. (1987) Regulation of the human interleukin-2 receptor alpha chain promoter: activation of a nonfunctional promoter by the transactivator gene of HTLV-I.. Cell, (10): [PMID:3030566]
5. Miedel, M C MC, Hulmes, J D JD, Weber, D V DV, Bailon, P P and Pan, Y C YC.. (1988) Structural analysis of recombinant soluble human interleukin-2 receptor. Primary structure, assignment of disulfide bonds and core IL-2 binding structure.. Biochemical and biophysical research communications, (15): [PMID:3134887]
6. Leonard, W J WJ and 9 more authors.. (1984) Molecular cloning and expression of cDNAs for the human interleukin-2 receptor.. Nature, [PMID:6090948]
7. Nikaido, T T and 8 more authors.. (1984) Molecular cloning of cDNA encoding human interleukin-2 receptor.. Nature, [PMID:6090949]
8. Bamborough, P P, Hedgecock, C J CJ and Richards, W G WG.. (1994) The interleukin-2 and interleukin-4 receptors studied by molecular modelling.. Structure (London, England : 1993), (15): [PMID:7529123]
9. Krouwels, F H FH and 5 more authors.. (1996) Glucocorticosteroids affect functions of airway- and blood-derived human T-cell clones, favoring the Th1 profile through two mechanisms.. American journal of respiratory cell and molecular biology, [PMID:8600944]
10. Berard, J L JL, Velez, R L RL, Freeman, R B RB and Tsunoda, S M SM.. (1999) A review of interleukin-2 receptor antagonists in solid organ transplantation.. Pharmacotherapy, [PMID:10512062]
11. Deloukas, P P and 135 more authors.. (2004) The DNA sequence and comparative analysis of human chromosome 10.. Nature, (27): [PMID:15164054]
12. Wang, Xinquan X, Rickert, Mathias M and Garcia, K Christopher KC.. (2005) Structure of the quaternary complex of interleukin-2 with its alpha, beta, and gammac receptors.. Science (New York, N.Y.), (18): [PMID:16293754]
13. Garcia, C D CD and 5 more authors.. () Plasmapheresis for recurrent posttransplant focal segmental glomerulosclerosis.. Transplantation proceedings, [PMID:16908318]
14. and Turturro, Francesco F.. (2007) Denileukin diftitox: a biotherapeutic paradigm shift in the treatment of lymphoid-derived disorders.. Expert review of anticancer therapy, [PMID:17187516]
15. and Waldmann, Thomas A.. (2007) Anti-Tac (daclizumab, Zenapax) in the treatment of leukemia, autoimmune diseases, and in the prevention of allograft rejection: a 25-year personal odyssey.. Journal of clinical immunology, [PMID:17216565]
16. and Waldmann, T A TA.. (2007) Daclizumab (anti-Tac, Zenapax) in the treatment of leukemia/lymphoma.. Oncogene, (28): [PMID:17530023]
17. Lowe, Christopher E CE and 12 more authors.. (2007) Large-scale genetic fine mapping and genotype-phenotype associations implicate polymorphism in the IL2RA region in type 1 diabetes.. Nature genetics, [PMID:17676041]
18. Duvic, Madeleine M and Talpur, Rakhshandra R.. (2008) Optimizing denileukin diftitox (Ontak) therapy.. Future oncology (London, England), [PMID:18684057]
19. Goudy, Kevin K and 18 more authors.. (2013) Human IL2RA null mutation mediates immunodeficiency with lymphoproliferation and autoimmunity.. Clinical immunology (Orlando, Fla.), [PMID:23416241]
20. Bezrodnik, L L, Caldirola, M S MS, Seminario, A G AG, Moreira, I I and Gaillard, M I MI.. (2014) Follicular bronchiolitis as phenotype associated with CD25 deficiency.. Clinical and experimental immunology, [PMID:24116927]
21. Chen, X X, Ji, Z L ZL and Chen, Y Z YZ.. (2002) TTD: Therapeutic Target Database.. Nucleic acids research, (1): [PMID:11752352]

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