Basic Information
ID: ALA3431457
Type: Functional
Description: GSK_TCAKS: pIC50 NIH 3T3 cell line viability assay using luminiscence. Tissue culture surface treated assay plates (1536-well) were used. 5uL of a 2 * 10^5 cells per mL solution were dispensed per well (~10^3 host cells), into plates already containing compounds which had been pre-dispensed. The 100% cells survival control was determined exposing the host-cells to a 0.5% DMSO concentration, whereas the 100% toxicity control was achieved by seeding the cells in presence of Amphotericin B at 50uM. Plates were incubated for four days at 37C, 5% CO2. The assay readout was developed by addition of 5uL of CellTiter-Glo Reagent following the manufacturer's instructions. Luminiscence was measured using a ViewLux.
Organism: Mus musculus
Cell type: NIH3T3
Activity Charts
Bioactivity
Activity Types for Assay ALA3431457
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 308.39 | 2.15 | 2.15 |
| 251.25 | -0.95 | -0.95 |
| 386.40 | 3.01 | 3.01 |
| 307.82 | 3.34 | 3.34 |
| 321.72 | 3.90 | 3.90 |
| 330.86 | 4.56 | 4.56 |
| 285.34 | 3.10 | 3.10 |
| 458.53 | 3.20 | 3.20 |
| 268.23 | -2.20 | -2.20 |
| 365.27 | 3.26 | 3.26 |
| 387.51 | 5.17 | 5.17 |
| 373.37 | 2.08 | 2.08 |
| 352.44 | 3.75 | 3.75 |
| 351.39 | 4.21 | 4.21 |
| 446.57 | 3.12 | 3.12 |
| 434.93 | 3.59 | 3.59 |
| 332.35 | 1.47 | 1.47 |
| 326.38 | 3.27 | 3.27 |
| 308.41 | 3.78 | 3.78 |
| 412.95 | 4.11 | 4.11 |
| 582.70 | 4.70 | 4.70 |
| 482.40 | 4.82 | 4.82 |
| 442.52 | 3.47 | 3.47 |
| 337.25 | 4.73 | 4.73 |
| 376.71 | 4.33 | 4.33 |
| 685.67 | 4.28 | 4.28 |
| 506.52 | 3.01 | 3.01 |
| 296.37 | 2.75 | 2.75 |
| 211.22 | 0.71 | 0.71 |
| 450.99 | 3.84 | 3.84 |
| 393.36 | 1.94 | 1.94 |
| 418.42 | 4.03 | 4.03 |
| 242.33 | 2.90 | 2.90 |
| 325.22 | 4.17 | 4.17 |
| 434.36 | 3.93 | 3.93 |
| 302.34 | 3.72 | 3.72 |
| 324.30 | 2.75 | 2.75 |
| 321.85 | 2.72 | 2.72 |
| 425.92 | 3.94 | 3.94 |
| 299.76 | 4.23 | 4.23 |
| 259.31 | 3.60 | 3.60 |
| 389.46 | 2.27 | 2.27 |
| 226.19 | 0.15 | 0.15 |
| 409.83 | 5.06 | 5.06 |
| 315.12 | 3.47 | 3.47 |
| 311.13 | 3.33 | 3.33 |
| 388.82 | 2.46 | 2.46 |
| 359.41 | 2.95 | 2.95 |
| 403.20 | 2.64 | 2.64 |
| 336.23 | 0.44 | 0.44 |
| 339.83 | 4.58 | 4.58 |
| 285.34 | 3.89 | 3.89 |
| 450.49 | 3.40 | 3.40 |
| 341.84 | 4.26 | 4.26 |
| 463.49 | 3.73 | 3.73 |
| 388.43 | 3.99 | 3.99 |
| 464.49 | 4.68 | 4.68 |
| 413.40 | 4.40 | 4.40 |
| 373.41 | 3.55 | 3.55 |
| 320.44 | 3.58 | 3.58 |
| 309.71 | 2.26 | 2.26 |
| 484.95 | 5.11 | 5.11 |
| 310.33 | 3.45 | 3.45 |
| 375.43 | 4.30 | 4.30 |
| 396.83 | 4.25 | 4.25 |
| 387.46 | 3.51 | 3.51 |
| 461.52 | 2.78 | 2.78 |
| 432.48 | 4.67 | 4.67 |
| 497.62 | 3.94 | 3.94 |
| 309.39 | 3.18 | 3.18 |
| 260.36 | 3.69 | 3.69 |
| 395.37 | 3.01 | 3.01 |
| 588.46 | 3.82 | 3.82 |
| 437.58 | 4.56 | 4.56 |
| 337.41 | 2.44 | 2.44 |
| 351.28 | 4.99 | 4.99 |
| 387.78 | 3.14 | 3.14 |
| 430.57 | 2.42 | 2.42 |
| 476.02 | 4.14 | 4.14 |
| 416.48 | 3.72 | 3.72 |
| 325.80 | 3.50 | 3.50 |
| 493.59 | 4.95 | 4.95 |
| 478.04 | 4.40 | 4.40 |
| 316.21 | 4.06 | 4.06 |
| 426.52 | 3.67 | 3.67 |
| 458.54 | 2.16 | 2.16 |
| 294.38 | 2.43 | 2.43 |
| 410.83 | 3.36 | 3.36 |
| 327.82 | 4.28 | 4.28 |
| 474.52 | 2.99 | 2.99 |
| 318.32 | 1.74 | 1.74 |
| 344.41 | 4.95 | 4.95 |
| 464.55 | 3.16 | 3.16 |
| 444.58 | 3.74 | 3.74 |
| 379.44 | 3.88 | 3.88 |
| 343.37 | 1.93 | 1.93 |
| 692.56 | 3.57 | 3.57 |
| 545.53 | 4.24 | 4.24 |
| 516.56 | 4.20 | 4.20 |
| 311.38 | 3.46 | 3.46 |
| 342.40 | 4.24 | 4.24 |
| 364.49 | 3.72 | 3.72 |
| 410.39 | 3.81 | 3.81 |
| 550.49 | 4.00 | 4.00 |
| 495.60 | 3.82 | 3.82 |
| 350.43 | 3.55 | 3.55 |
| 302.41 | 2.65 | 2.65 |
| 540.54 | 2.73 | 2.73 |
| 396.42 | 2.99 | 2.99 |
| 464.52 | 3.61 | 3.61 |
| 442.57 | 3.33 | 3.33 |
| 383.86 | 3.68 | 3.68 |
| 303.79 | 3.23 | 3.23 |
| 383.45 | 4.21 | 4.21 |
| 394.52 | 4.57 | 4.57 |
| 457.55 | 2.31 | 2.31 |
| 473.62 | 4.18 | 4.18 |
| 435.50 | 3.78 | 3.78 |
| 401.65 | 4.02 | 4.02 |
| 412.54 | 4.73 | 4.73 |
| 348.43 | 4.71 | 4.71 |
| 422.50 | 4.60 | 4.60 |
| 395.48 | 2.77 | 2.77 |
| 335.41 | 2.17 | 2.17 |
| 464.52 | 5.55 | 5.55 |
| 381.46 | 4.32 | 4.32 |
| 573.57 | 2.30 | 2.30 |
| 495.48 | 2.45 | 2.45 |
| 810.62 | 2.88 | 2.88 |
| 472.56 | 5.15 | 5.15 |
| 499.49 | 4.49 | 4.49 |
| 422.50 | 2.42 | 2.42 |
| 335.82 | 3.64 | 3.64 |
| 400.91 | 2.53 | 2.53 |
| 387.23 | 3.27 | 3.27 |
| 524.54 | 4.11 | 4.11 |
| 459.38 | 3.33 | 3.33 |
| 348.36 | 3.18 | 3.18 |
| 602.04 | 4.36 | 4.36 |
| 343.82 | 3.14 | 3.14 |
| 284.34 | 3.42 | 3.42 |
| 213.22 | 1.40 | 1.40 |
| 335.45 | 3.22 | 3.22 |
| 411.40 | 3.18 | 3.18 |
| 289.77 | 2.75 | 2.75 |
| 357.85 | 2.02 | 2.02 |
| 342.42 | 3.52 | 3.52 |
| 424.03 | 3.63 | 3.63 |
| 429.50 | 4.89 | 4.89 |
| 453.97 | 3.29 | 3.29 |
| 353.45 | 4.03 | 4.03 |
| 338.41 | 3.69 | 3.69 |
| 350.45 | 4.33 | 4.33 |
| 354.39 | 3.33 | 3.33 |
| 379.36 | 4.42 | 4.42 |
| 228.28 | 2.60 | 2.60 |
| 469.37 | 2.86 | 2.86 |
| 350.42 | 3.48 | 3.48 |
| 346.43 | 3.58 | 3.58 |
| 334.37 | 2.99 | 2.99 |
| 350.42 | 3.87 | 3.87 |
| 328.37 | 1.82 | 1.82 |
| 295.35 | 2.56 | 2.56 |
| 346.89 | 4.04 | 4.04 |
| 328.20 | 2.77 | 2.77 |
| 327.43 | 3.13 | 3.13 |
| 365.52 | 3.92 | 3.92 |
| 330.39 | 3.58 | 3.58 |
| 322.37 | 1.94 | 1.94 |
| 351.41 | 2.52 | 2.52 |
| 326.40 | 1.90 | 1.90 |
| 588.61 | 3.63 | 3.63 |
| 343.43 | 4.02 | 4.02 |
| 326.42 | 3.47 | 3.47 |
| 325.46 | 3.03 | 3.03 |
| 349.41 | 3.54 | 3.54 |
| 338.24 | 3.50 | 3.50 |
| 518.97 | 2.95 | 2.95 |
| 400.53 | 4.53 | 4.53 |
| 421.24 | 2.97 | 2.97 |
| 318.69 | 3.69 | 3.69 |
| 410.95 | 4.18 | 4.18 |
| 501.38 | 2.30 | 2.30 |
| 315.80 | 4.49 | 4.49 |
| 461.49 | 4.02 | 4.02 |
| 469.63 | 3.47 | 3.47 |
| 489.61 | 2.72 | 2.72 |
| 241.04 | 2.54 | 2.54 |
| 433.31 | 3.51 | 3.51 |
| 292.30 | 3.42 | 3.42 |
| 518.89 | 5.15 | 5.15 |
| 554.59 | 3.26 | 3.26 |
| 615.59 | 3.32 | 3.32 |
| 356.84 | 3.92 | 3.92 |
| 391.45 | 4.96 | 4.96 |
| 321.34 | 3.89 | 3.89 |
| 517.94 | 5.30 | 5.30 |
| 316.36 | 3.64 | 3.64 |
| 369.60 | 4.48 | 4.48 |
| 419.66 | 4.16 | 4.16 |
| 374.87 | 4.22 | 4.22 |
| 387.48 | 4.01 | 4.01 |
| 480.48 | 3.78 | 3.78 |
| 311.41 | 4.02 | 4.02 |
| 393.78 | 3.03 | 3.03 |
| 306.82 | 3.72 | 3.72 |
| 324.41 | 3.33 | 3.33 |
| 569.06 | 2.07 | 2.07 |
| 231.30 | 1.70 | 1.70 |
| 274.66 | 3.91 | 3.91 |
| 304.78 | 2.83 | 2.83 |
| 225.32 | 1.85 | 1.85 |
| 419.53 | 4.63 | 4.63 |
| 546.67 | 5.73 | 5.73 |
| 385.77 | 2.71 | 2.71 |
| 446.53 | 4.65 | 4.65 |
| 418.52 | 3.13 | 3.13 |
| 423.97 | 2.79 | 2.79 |
| 407.97 | 3.35 | 3.35 |
| 553.44 | 2.99 | 2.99 |
| 222.25 | 3.00 | 3.00 |
| 426.90 | 3.34 | 3.34 |