Basic Information
ID: ALA3705072
Type: Binding
Description: Inhibition Assay: The inhibition of full-length hepatitis C NS3 protease enzyme was measured essentially as described in Poliakov, 2002 Prot Expression & Purification 25 363 371. Briefly, the hydrolysis of a depsipeptide substrate, Ac-DED(Edans)EEAbu[COO]ASK(Dabcyl)-NH2 (AnaSpec, San Jose, USA), was measured spectrofluorometrically in the presence of a peptide cofactor, KKGSVVIVGRIVLSGK (Ake Engstrom, Department of Medical Biochemistry and Microbiology, Uppsala University, Sweden). [Landro, 1997 #Biochem 36 9340-9348]. The enzyme (1 nM) was incubated in 50 mM HEPES, pH 7.5, 10 mM DTT, 40% glycerol, 0.1% n-octyl-D-glucoside, with 25 μM NS4A cofactor and inhibitor at 30 C. for 10 min, whereupon the reaction was initiated by addition of 0.5 μM substrate. Inhibitors were dissolved in DMSO, sonicated for 30 sec. and vortexed.
Organism: Hepatitis C virus
Activity Charts
Bioactivity
Activity Types for Assay ALA3705072
Ki
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 749.96 | 5.25 | 5.25 |
| 750.94 | 4.94 | 4.94 |
| 771.36 | 5.29 | 5.29 |
| 785.39 | 5.68 | 5.68 |
| 667.23 | 6.08 | 6.08 |
| 770.37 | 5.60 | 5.60 |
| 784.40 | 5.99 | 5.99 |
| 763.98 | 5.64 | 5.64 |
| 749.96 | 5.30 | 5.30 |
| 646.81 | 5.78 | 5.78 |