Basic Information
ID: ALA3705073
Type: Binding
Description: In Vitro Kinase Assay: PDK-1 kinase activity This in vitro assay was performed using a PDK-1 kinase assay kit (Upstate, Lake Placid, N.Y.) according to the vendor's instructions. This cell-free assay is based on the ability of recombinant PDK-1, in the presence of DMSO vehicle or the test agent, to activate its downstream kinase serum- and glucocorticoid-regulated kinase (SGK.) which, in turn, phosphorylates the Akt/SGK-specific peptide substrate RPRAATF with [γ-32]-ATP. The [32P]-phosphorylated peptide substrate was then separated from the residual [γ-32P]-ATP using PS1 phosphocellulose paper and quantitated by a scintillation counter after three washes with 0.75% phosphoric acid.
Organism: Homo sapiens
Activity Charts
Bioactivity
Activity Types for Assay ALA3705073
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 381.38 | 3.51 | 3.51 |
| 406.39 | 3.69 | 3.69 |
| 460.46 | 5.76 | 5.76 |
| 477.90 | 5.53 | 5.53 |
| 546.79 | 6.83 | 6.83 |
| 467.47 | 5.51 | 5.51 |
| 445.45 | 5.77 | 5.77 |
| 417.41 | 4.36 | 4.36 |
| 474.30 | 4.14 | 4.14 |
| 488.33 | 4.53 | 4.53 |
| 436.42 | 4.06 | 4.06 |
| 450.45 | 4.45 | 4.45 |
| 423.46 | 4.55 | 4.55 |
| 423.46 | 4.50 | 4.50 |
| 443.45 | 4.87 | 4.87 |
| 512.34 | 6.18 | 6.18 |
| 512.34 | 6.18 | 6.18 |
| 457.48 | 5.18 | 5.18 |
| 511.45 | 5.89 | 5.89 |
| 536.37 | 5.77 | 5.77 |
| 471.50 | 5.49 | 5.49 |
| 499.56 | 6.22 | 6.22 |
| 499.56 | 6.17 | 6.17 |
| 457.48 | 4.80 | 4.80 |
| 455.46 | 4.78 | 4.78 |
| 467.47 | 5.51 | 5.51 |
| 467.47 | 5.51 | 5.51 |
| 431.42 | 5.96 | 5.96 |
| 413.40 | 6.74 | 6.74 |
| 446.43 | 6.17 | 6.17 |
| 456.43 | 6.04 | 6.04 |
| 431.42 | 7.13 | 7.13 |
| 430.43 | 7.40 | 7.40 |
| 427.43 | 6.93 | 6.93 |
| 470.46 | 5.97 | 5.97 |
| 446.43 | 6.36 | 6.36 |
| 461.47 | 6.06 | 6.06 |