Basic Information
ID: ALA3705108
Type: Binding
Description: ALDH2 Assay: Standard ALDH2 reaction mixtures contained 150 uM formaldehyde, 2.5 mM NAD+, 10 mM MgCl2 and 10 nM recombinant human ALDH2 in 50 mM Hepes buffer, pH 7.4, 0.01% Tween 20 in a final volume of 50 ul using 384-well plates. After 60 min of pre-incubation of compound with ALDH2 and formaldehyde, the reaction was started by adding NAD+ and the reaction mixture was allowed to proceed for 90 minutes. Activity of the enzyme was determined by monitoring NADH formation using Perkin-Elmer Envision Reader with excitation and emission wavelengths set at 340 and 460 nm, respectively.
Organism: Homo sapiens
Activity Charts
Bioactivity
Activity Types for Assay ALA3705108
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 379.44 | 3.59 | 3.59 |
| 415.47 | 4.23 | 4.23 |
| 397.45 | 2.95 | 2.95 |
| 383.43 | 2.83 | 2.83 |
| 437.52 | 3.88 | 3.88 |
| 436.53 | 3.84 | 3.84 |
| 416.46 | 3.63 | 3.63 |
| 417.45 | 3.02 | 3.02 |
| 416.46 | 3.63 | 3.63 |
| 409.46 | 3.36 | 3.36 |
| 423.49 | 3.49 | 3.49 |
| 522.62 | 4.83 | 4.83 |
| 494.57 | 4.05 | 4.05 |
| 432.46 | 2.92 | 2.92 |
| 462.53 | 3.05 | 3.05 |
| 422.51 | 3.18 | 3.18 |
| 490.58 | 3.83 | 3.83 |
| 433.49 | 3.27 | 3.27 |
| 447.52 | 4.01 | 4.01 |
| 419.46 | 2.96 | 2.96 |
| 394.45 | 2.40 | 2.40 |
| 506.58 | 4.19 | 4.19 |
| 423.49 | 3.61 | 3.61 |
| 480.54 | 2.68 | 2.68 |
| 512.53 | 3.69 | 3.69 |
| 493.59 | 2.59 | 2.59 |
| 452.49 | 1.63 | 1.63 |
| 506.58 | 2.80 | 2.80 |
| 480.54 | 2.41 | 2.41 |
| 492.55 | 2.68 | 2.68 |
| 466.52 | 2.02 | 2.02 |
| 519.62 | 2.98 | 2.98 |
| 498.51 | 3.30 | 3.30 |
| 476.55 | 3.44 | 3.44 |
| 476.55 | 3.44 | 3.44 |
| 591.69 | 4.09 | 4.09 |
| 577.66 | 3.70 | 3.70 |
| 591.69 | 4.04 | 4.04 |
| 450.56 | 3.02 | 3.02 |
| 423.49 | 2.73 | 2.73 |
| 419.46 | 2.21 | 2.21 |