Basic Information
ID: ALA3705142
Type: Binding
Description: Binding Assay: SSTR5 binding assays can be performed by labeling somatostatin and determining the ability of a compound to inhibit somatostatin binding. (Poitout et al., J. Med. Chem. 44:29900-3000, (2001); Hocart et al., J. Med. Chem. 41:1146-1154, (1998); J. Med. Chem. 50, 6292-6295 (2007) and J. Med. Chem. 50, 6295-6298 (2007)). Binding assays were performed using (3-125I-Tyr11)-SRIF-14 or (3-125I-Tyr11)-SRIF-28 as the radioligand (used at 0.1 nM) and The Packard Unifilter assay plate. The assay buffer consisted of 50 mM TrisHCl (pH 7.8) with 1 mM EGTA, 5 in M MgCl2, leupeptin (10 μg/mL), pepstatin (10 μg/mL), bacitracin (200 μg/mL), and aprotinin (0.5 μg/mL).
Organism: Homo sapiens
Activity Charts
Bioactivity
Activity Types for Assay ALA3705142
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 542.56 | 5.41 | 5.41 |
| 567.06 | 6.54 | 6.54 |
| 508.58 | 5.23 | 5.23 |
| 502.57 | 4.58 | 4.58 |
| 582.62 | 6.76 | 6.76 |
| 590.60 | 6.26 | 6.26 |
| 542.56 | 5.41 | 5.41 |
| 542.56 | 5.41 | 5.41 |
| 538.60 | 5.24 | 5.24 |
| 518.61 | 5.00 | 5.00 |
| 573.04 | 6.23 | 6.23 |
| 552.63 | 5.63 | 5.63 |
| 593.46 | 6.58 | 6.58 |
| 518.61 | 5.00 | 5.00 |
| 562.55 | 5.65 | 5.65 |
| 528.53 | 5.24 | 5.24 |
| 542.56 | 5.46 | 5.46 |
| 568.60 | 6.16 | 6.16 |
| 544.56 | 5.51 | 5.51 |
| 620.58 | 5.42 | 5.42 |
| 538.60 | 5.24 | 5.24 |
| 598.62 | 6.64 | 6.64 |
| 582.62 | 6.76 | 6.76 |
| 608.66 | 6.65 | 6.65 |
| 612.61 | 6.80 | 6.80 |
| 592.57 | 6.13 | 6.13 |
| 541.02 | 5.79 | 5.79 |
| 583.04 | 5.41 | 5.41 |
| 524.57 | 5.27 | 5.27 |
| 559.01 | 5.93 | 5.93 |
| 536.60 | 5.14 | 5.14 |
| 568.60 | 6.16 | 6.16 |
| 561.01 | 6.02 | 6.02 |
| 506.58 | 5.00 | 5.00 |
| 456.54 | 3.73 | 3.73 |
| 556.59 | 5.72 | 5.72 |
| 555.05 | 6.08 | 6.08 |
| 596.99 | 6.30 | 6.30 |
| 556.59 | 5.38 | 5.38 |
| 568.47 | 5.22 | 5.22 |
| 560.55 | 5.55 | 5.55 |
| 543.55 | 4.81 | 4.81 |
| 591.03 | 6.03 | 6.03 |
| 577.00 | 5.82 | 5.82 |
| 577.00 | 5.64 | 5.64 |
| 611.02 | 6.22 | 6.22 |
| 500.48 | 4.19 | 4.19 |
| 500.48 | 4.19 | 4.19 |
| 611.02 | 6.36 | 6.36 |
| 611.02 | 6.36 | 6.36 |
| 540.57 | 5.11 | 5.11 |
| 528.53 | 5.07 | 5.07 |
| 562.55 | 5.65 | 5.65 |
| 568.60 | 6.16 | 6.16 |
| 514.50 | 4.63 | 4.63 |
| 433.51 | 4.26 | 4.26 |
| 556.59 | 5.80 | 5.80 |
| 562.64 | 6.37 | 6.37 |
| 536.58 | 6.11 | 6.11 |
| 532.64 | 5.39 | 5.39 |
| 552.63 | 5.63 | 5.63 |
| 534.58 | 5.59 | 5.59 |
| 528.53 | 5.02 | 5.02 |
| 548.61 | 5.98 | 5.98 |
| 521.59 | 5.38 | 5.38 |
| 548.61 | 5.98 | 5.98 |
| 549.60 | 5.37 | 5.37 |
| 549.60 | 5.37 | 5.37 |
| 590.69 | 6.64 | 6.64 |
| 602.67 | 5.47 | 5.47 |
| 562.64 | 6.29 | 6.29 |
| 583.06 | 6.63 | 6.63 |
| 588.68 | 6.40 | 6.40 |
| 549.60 | 5.37 | 5.37 |
| 572.64 | 5.46 | 5.46 |
| 521.59 | 4.97 | 4.97 |
| 548.61 | 5.98 | 5.98 |
| 616.61 | 7.00 | 7.00 |
| 562.64 | 6.29 | 6.29 |
| 549.60 | 5.37 | 5.37 |
| 566.60 | 6.12 | 6.12 |
| 583.06 | 6.63 | 6.63 |
| 578.64 | 5.99 | 5.99 |
| 566.60 | 6.12 | 6.12 |
| 540.54 | 5.86 | 5.86 |
| 541.53 | 5.25 | 5.25 |
| 522.55 | 5.72 | 5.72 |
| 522.55 | 5.72 | 5.72 |
| 540.54 | 5.86 | 5.86 |
| 554.57 | 6.25 | 6.25 |
| 554.57 | 6.17 | 6.17 |
| 568.59 | 6.48 | 6.48 |
| 564.48 | 6.48 | 6.48 |
| 529.49 | 5.60 | 5.60 |
| 554.57 | 6.17 | 6.17 |
| 568.59 | 6.48 | 6.48 |
| 554.57 | 6.17 | 6.17 |
| 519.57 | 5.28 | 5.28 |
| 538.58 | 5.64 | 5.64 |
| 554.57 | 6.25 | 6.25 |
| 580.48 | 6.36 | 6.36 |
| 584.59 | 6.18 | 6.18 |
| 536.55 | 5.95 | 5.95 |
| 569.58 | 6.16 | 6.16 |
| 572.56 | 6.31 | 6.31 |
| 589.01 | 6.82 | 6.82 |
| 506.00 | 5.71 | 5.71 |
| 544.96 | 6.42 | 6.42 |
| 509.97 | 5.54 | 5.54 |
| 559.97 | 6.42 | 6.42 |
| 493.59 | 4.90 | 4.90 |
| 504.59 | 4.58 | 4.58 |
| 450.54 | 4.35 | 4.35 |
| 490.56 | 4.94 | 4.94 |
| 504.58 | 5.33 | 5.33 |
| 519.60 | 5.03 | 5.03 |
| 525.00 | 5.59 | 5.59 |
| 476.53 | 4.55 | 4.55 |
| 525.00 | 5.59 | 5.59 |
| 529.52 | 5.34 | 5.34 |
| 529.52 | 5.34 | 5.34 |
| 450.54 | 4.44 | 4.44 |
| 478.59 | 5.21 | 5.21 |
| 450.54 | 4.48 | 4.48 |
| 464.56 | 4.74 | 4.74 |
| 468.53 | 4.58 | 4.58 |
| 484.98 | 5.09 | 5.09 |
| 450.54 | 4.57 | 4.57 |
| 410.43 | 3.10 | 3.10 |
| 436.51 | 4.09 | 4.09 |
| 424.43 | 3.39 | 3.39 |
| 436.47 | 3.26 | 3.26 |
| 478.55 | 4.14 | 4.14 |
| 480.54 | 4.67 | 4.67 |
| 466.51 | 4.45 | 4.45 |
| 478.55 | 4.32 | 4.32 |
| 473.53 | 4.01 | 4.01 |
| 491.55 | 3.41 | 3.41 |
| 473.53 | 4.18 | 4.18 |
| 494.57 | 4.91 | 4.91 |
| 506.60 | 4.78 | 4.78 |
| 476.53 | 4.07 | 4.07 |
| 512.57 | 4.72 | 4.72 |
| 554.65 | 5.89 | 5.89 |
| 534.61 | 4.35 | 4.35 |
| 477.56 | 4.92 | 4.92 |
| 514.99 | 5.27 | 5.27 |
| 553.66 | 6.49 | 6.49 |
| 449.51 | 3.88 | 3.88 |
| 477.56 | 4.75 | 4.75 |
| 491.59 | 5.14 | 5.14 |
| 435.48 | 3.87 | 3.87 |
| 511.58 | 5.33 | 5.33 |
| 577.60 | 7.00 | 7.00 |
| 526.43 | 5.67 | 5.67 |
| 477.56 | 4.92 | 4.92 |
| 562.00 | 6.49 | 6.49 |
| 561.70 | 6.56 | 6.56 |
| 535.67 | 5.81 | 5.81 |
| 563.53 | 6.12 | 6.12 |
| 525.96 | 5.70 | 5.70 |
| 525.63 | 6.17 | 6.17 |
| 463.56 | 4.81 | 4.81 |
| 509.56 | 5.70 | 5.70 |
| 559.61 | 6.21 | 6.21 |
| 536.51 | 5.11 | 5.11 |
| 482.54 | 4.69 | 4.69 |
| 420.47 | 3.33 | 3.33 |
| 550.54 | 5.42 | 5.42 |