Basic Information
ID: ALA3705176
Type: Binding
Description: Binding Assay: Methods for performing in vitro dopamine receptor binding studies are described in Huang et al. J. Med. Chem. 44:1815-1826 (2001) and Luedtke et al. Synapse 38:438-439 (2000). These papers describe radioactively labeled dopamine receptor selective ligands binding with picomolar affinity and nonselectivity to D2 and D3 dopamine receptors expressed in Sf9 and HEK 293 cells. 125I-IABN binds with 7- to 10-fold lower affinity to human D4.4 dopamine receptors expressed in HEK 293 cells. Dissociation constants (Kd) calculated from kinetic experiments were found to be in agreement with equilibrium Kd values obtained from saturation binding studies. Saturation plots of the binding of 125I-IABN with rat caudate membrane preparations were monophasic and exhibited low nonspecific binding.
Organism: Homo sapiens
Cell type: Sf9
Activity Charts
Bioactivity
Activity Types for Assay ALA3705176
Ki
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 497.43 | 4.46 | 4.46 |
| 495.45 | 5.47 | 5.47 |
| 495.45 | 5.47 | 5.47 |
| 497.43 | 4.40 | 4.40 |
| 497.43 | 5.18 | 5.18 |
| 442.56 | 3.87 | 3.87 |
| 453.59 | 4.37 | 4.37 |
| 499.44 | 4.36 | 4.36 |
| 510.47 | 4.87 | 4.87 |
| 460.58 | 3.06 | 3.06 |
| 471.60 | 3.57 | 3.57 |
| 541.48 | 4.93 | 4.93 |
| 499.44 | 4.36 | 4.36 |
| 510.47 | 4.87 | 4.87 |
| 471.60 | 3.57 | 3.57 |
| 461.39 | 3.78 | 3.78 |
| 422.53 | 2.48 | 2.48 |
| 450.58 | 3.26 | 3.26 |
| 462.38 | 4.04 | 4.04 |
| 423.51 | 2.74 | 2.74 |
| 588.27 | 4.65 | 4.65 |
| 549.41 | 3.35 | 3.35 |
| 439.58 | 3.21 | 3.21 |
| 461.39 | 3.78 | 3.78 |
| 422.53 | 2.48 | 2.48 |
| 480.37 | 4.18 | 4.18 |
| 441.50 | 2.88 | 2.88 |
| 492.40 | 4.05 | 4.05 |
| 453.54 | 2.75 | 2.75 |