Basic Information
ID: ALA3705193
Type: Binding
Description: FRET Assay: We have developed effective assays to monitor the inhibition of CBFβ-SMMHC binding to the Runt domain (as well as for CBFβ binding to the Runt domain). We fused the green fluorescent protein derivative Cerulean to the N-terminus of the Runt domain and the green fluorescent protein derivative Venus to the N-terminus of CBFβ-SMMHC (as well as to CBFβ (Gorczynski, Grembecka et al. 2007)). The ratio of the emission intensities at 525 nm and 474 nm, measured after excitation at 433 nm, was used as the readout in this assay. The dynamic range for the FRET assay was determined by adding a 30-fold excess of untagged CBFβ-SMMHC (or CBFβ in the case of CBFβ Runt domain binding) to the assay and the associated change in the FRET ratio was defined as 100% inhibition.
Organism: Homo sapiens
Activity Charts
Bioactivity
Activity Types for Assay ALA3705193
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 261.29 | 2.81 | 2.81 |
| 229.67 | 3.28 | 3.28 |
| 209.25 | 2.93 | 2.93 |
| 223.28 | 3.24 | 3.24 |
| 213.22 | 2.76 | 2.76 |
| 225.25 | 2.63 | 2.63 |
| 209.25 | 2.93 | 2.93 |
| 225.25 | 2.12 | 2.12 |
| 239.28 | 3.02 | 3.02 |
| 239.28 | 3.02 | 3.02 |
| 253.31 | 3.41 | 3.41 |
| 211.22 | 2.33 | 2.33 |
| 255.28 | 1.61 | 1.61 |
| 241.32 | 3.05 | 3.05 |
| 273.32 | 2.03 | 2.03 |
| 279.22 | 3.52 | 3.52 |
| 223.28 | 3.24 | 3.24 |
| 231.21 | 2.90 | 2.90 |
| 249.19 | 3.04 | 3.04 |
| 261.23 | 2.40 | 2.40 |
| 259.70 | 2.77 | 2.77 |
| 264.12 | 3.93 | 3.93 |
| 263.22 | 3.64 | 3.64 |
| 297.67 | 4.30 | 4.30 |
| 280.33 | 2.46 | 2.46 |
| 239.23 | 2.16 | 2.16 |
| 356.31 | 4.59 | 4.59 |
| 255.28 | 2.13 | 2.13 |
| 299.33 | 2.14 | 2.14 |
| 353.30 | 3.55 | 3.55 |
| 255.28 | 2.13 | 2.13 |
| 297.21 | 3.66 | 3.66 |
| 255.28 | 2.13 | 2.13 |
| 269.26 | 2.17 | 2.17 |