Assay Report Card

Basic Information

ID: ALA3705203

Type: Binding

Description: In Vitro Ezyme Assay: 5 uL of each compound dilution were robotically pipetted to Costar serocluster plates maintaining the same plate layout. All assay mixtures consisted of the following volumes: 5 uL diluted compound, 10 uL target enzyme preparation, 1 uL substrate, 5 uL assay ATP. From each assay mixture, 10 uL of assay mixture was spotted onto Millipore Multiscreen-PH opaque plates and washed twice for 10 minutes in 1% phosphoric acid. The plates were dried at 40 C. for 30 minutes, then substrate-phosphate complexes were quantitated by scintillation counting. These Millipore plates are in a 96-well format with immobilized P81 phosphocellulose membranes in the wells. Both the phosphorylated and non-phosphorylated form of the substrate bind to the membrane while ATP (unincorporated phosphate) is removed by subsequent wash steps.

Organism: Homo sapiens

Activity Charts

Bioactivity

Activity Types for Assay ALA3705203

IC50

Total(151)

Compound Summary

Parent Molecular Weight	ALogP	Polar Surface Area
309.35	3.76	3.76
310.39	3.06	3.06
230.30	2.73	2.73
278.31	2.21	2.21
260.32	2.07	2.07
260.32	2.07	2.07
201.25	2.69	2.69
280.33	1.33	1.33
313.34	1.06	1.06
363.35	1.64	1.64
306.32	2.50	2.50
341.39	1.62	1.62
274.31	2.28	2.28
288.33	2.36	2.36
323.40	1.49	1.49
415.50	2.78	2.78
288.33	2.36	2.36
215.28	2.99	2.99
293.35	4.06	4.06
231.28	1.85	1.85
309.38	1.18	1.18
327.37	1.36	1.36
371.42	0.99	0.99
327.37	1.36	1.36
371.42	0.99	0.99
418.48	2.59	2.59
246.29	2.28	2.28
323.40	1.49	1.49
273.32	1.68	1.68
309.38	1.22	1.22
353.43	0.85	0.85
367.46	1.50	1.50
417.49	3.19	3.19
419.56	3.16	3.16
433.95	3.71	3.71
429.53	3.06	3.06
399.50	3.06	3.06
413.53	3.10	3.10
428.54	2.68	2.68
422.54	1.19	1.19
391.40	2.42	2.42
403.49	1.82	1.82
414.52	2.64	2.64
400.49	2.45	2.45
380.50	1.42	1.42
394.53	1.81	1.81
415.50	2.39	2.39
372.44	1.98	1.98
275.34	1.67	1.67
315.40	2.59	2.59
303.39	1.66	1.66
274.35	2.19	2.19
289.36	2.06	2.06
337.38	2.21	2.21
414.52	2.84	2.84
337.40	4.33	4.33
293.35	4.06	4.06
248.29	2.72	2.72
288.38	2.58	2.58
303.39	2.45	2.45
317.37	2.55	2.55
469.58	4.27	4.27
358.37	4.22	4.22
380.45	4.69	4.69
358.37	4.22	4.22
385.38	3.99	3.99
358.37	4.22	4.22
370.41	4.09	4.09
375.34	3.58	3.58
330.34	3.68	3.68
331.33	3.07	3.07
278.31	2.72	2.72
340.38	4.08	4.08
348.41	1.83	1.83
341.37	3.48	3.48
373.42	2.79	2.79
248.29	2.72	2.72
262.31	3.02	3.02
248.29	2.72	2.72
260.32	2.59	2.59
284.27	3.29	3.29
327.18	3.48	3.48
309.19	3.34	3.34
264.74	3.23	3.23
305.38	2.78	2.78
287.39	2.64	2.64
234.26	2.41	2.41
248.29	2.72	2.72
308.39	1.98	1.98
260.32	2.59	2.59
330.42	1.69	1.69
261.27	2.18	2.18
294.77	3.24	3.24
312.76	3.38	3.38
400.49	2.53	2.53
351.44	3.63	3.63
216.27	2.27	2.27
231.28	1.85	1.85
301.42	2.76	2.76
287.39	2.42	2.42
315.45	3.15	3.15
301.42	2.81	2.81
337.36	3.84	3.84
256.33	3.15	3.15
244.32	2.83	2.83
282.33	3.53	3.53
230.30	2.58	2.58
262.36	2.99	2.99
292.37	3.94	3.94
300.39	1.68	1.68
299.40	2.74	2.74
293.36	3.33	3.33
293.36	3.33	3.33
285.38	2.87	2.87
298.40	4.00	4.00
311.37	2.60	2.60
309.36	2.74	2.74
302.41	2.24	2.24
302.41	2.29	2.29
289.36	2.33	2.33
328.44	2.78	2.78
316.43	2.63	2.63
339.43	2.97	2.97
374.47	4.40	4.40
328.44	2.68	2.68
344.44	1.66	1.66
259.34	2.34	2.34
322.40	2.77	2.77
375.82	4.13	4.13
273.37	2.16	2.16
307.38	3.52	3.52
304.35	3.29	3.29
284.27	2.99	2.99
298.29	3.60	3.60
273.37	2.30	2.30
329.43	2.41	2.41
313.43	3.17	3.17
342.47	2.32	2.32
392.51	1.51	1.51
287.35	2.54	2.54
316.39	1.82	1.82
323.38	3.74	3.74
394.48	0.99	0.99
364.41	1.96	1.96
274.35	1.64	1.64
350.42	2.39	2.39
364.45	2.78	2.78
322.40	3.59	3.59
355.43	2.65	2.65
363.47	2.27	2.27
346.41	4.14	4.14