Basic Information
ID: ALA3705228
Type: Binding
Description: Enzyme Assay: The aim of this assay is to determine the affinity of a test compound for the mPGES-1 enzyme. 47 ul of recombinant human mPGES-1 (0.5 ug protein/well) containing microsomal suspension in a buffer containing GSH, (2.5 mmol/L L-Glutathione reduced, dissolved in 0.1 mol/L Phosphat Buffer pH 7.4) is dispensed in a 384-well plate and thereafter 1 ul of the test compound(s) is/are added and incubated for 25 minutes at room temperature. The enzyme reaction is started by the addition of 2 ul PGH2 (final conc. 2 uM) dissolved in water-free Diglyme. After 60 seconds the reaction is terminated by addition of a stop solution containing FeCl2 (10 uL 0.074 mol/l FeCl2). The samples are diluted between 1:25 in PBS (Phosphate Buffered Saline). 10 ul of the diluted samples are transferred to 384-well low volume plate.
Format: BAO_0000019
Organism: Homo sapiens
Target: Prostaglandin E synthase(ALA5658)
Document: ALA3639137
BindingDB Source: 6363
Activity Charts
Bioactivity
Activity Types for Assay ALA3705228
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 678.53 | 7.78 | 7.78 |
| 664.50 | 7.77 | 7.77 |
| 616.49 | 7.28 | 7.28 |
| 669.35 | 7.93 | 7.93 |
| 683.38 | 7.94 | 7.94 |
| 650.47 | 6.92 | 6.92 |
| 621.34 | 7.44 | 7.44 |
| 666.93 | 7.43 | 7.43 |
| 588.43 | 6.41 | 6.41 |
| 583.58 | 6.25 | 6.25 |
| 676.51 | 7.71 | 7.71 |
| 602.46 | 7.27 | 7.27 |
| 694.53 | 8.14 | 8.14 |
| 672.92 | 8.34 | 8.34 |
| 626.41 | 6.58 | 6.58 |
| 594.88 | 7.47 | 7.47 |
| 576.89 | 7.33 | 7.33 |
| 594.88 | 7.47 | 7.47 |
| 556.42 | 5.95 | 5.95 |
| 548.37 | 5.72 | 5.72 |
| 611.33 | 7.98 | 7.98 |
| 594.88 | 7.47 | 7.47 |
| 547.94 | 6.43 | 6.43 |
| 645.62 | 6.75 | 6.75 |
| 624.01 | 6.95 | 6.95 |
| 577.50 | 5.19 | 5.19 |
| 629.50 | 7.05 | 7.05 |
| 656.92 | 7.97 | 7.97 |
| 638.93 | 7.84 | 7.84 |
| 622.48 | 7.32 | 7.32 |
| 582.48 | 6.21 | 6.21 |
| 656.92 | 7.97 | 7.97 |
| 696.01 | 8.45 | 8.45 |
| 686.51 | 7.88 | 7.88 |
| 704.50 | 8.02 | 8.02 |
| 612.51 | 5.84 | 5.84 |
| 660.42 | 6.86 | 6.86 |
| 624.44 | 6.61 | 6.61 |
| 656.92 | 7.97 | 7.97 |
| 645.95 | 7.29 | 7.29 |
| 687.50 | 7.27 | 7.27 |
| 592.42 | 5.92 | 5.92 |
| 651.37 | 6.85 | 6.85 |
| 656.92 | 7.97 | 7.97 |
| 596.51 | 6.46 | 6.46 |
| 673.47 | 7.60 | 7.60 |
| 624.44 | 6.61 | 6.61 |
| 654.59 | 6.87 | 6.87 |
| 668.55 | 7.03 | 7.03 |
| 596.51 | 6.46 | 6.46 |
| 624.56 | 7.24 | 7.24 |
| 582.48 | 6.07 | 6.07 |
| 652.96 | 8.14 | 8.14 |
| 622.54 | 6.85 | 6.85 |
| 556.44 | 5.68 | 5.68 |
| 632.54 | 6.90 | 6.90 |
| 610.41 | 6.22 | 6.22 |
| 644.55 | 7.67 | 7.67 |
| 673.38 | 8.49 | 8.49 |
| 673.47 | 7.60 | 7.60 |
| 649.55 | 7.42 | 7.42 |
| 653.95 | 7.54 | 7.54 |
| 646.51 | 7.24 | 7.24 |
| 639.92 | 7.23 | 7.23 |
| 673.47 | 7.60 | 7.60 |
| 673.47 | 7.60 | 7.60 |
| 660.54 | 7.48 | 7.48 |
| 624.01 | 6.95 | 6.95 |
| 617.46 | 7.15 | 7.15 |
| 589.57 | 6.29 | 6.29 |
| 656.92 | 7.97 | 7.97 |
| 640.47 | 7.46 | 7.46 |
| 640.47 | 7.46 | 7.46 |
| 662.08 | 7.27 | 7.27 |
| 593.96 | 5.71 | 5.71 |
| 650.48 | 7.73 | 7.73 |
| 700.48 | 8.21 | 8.21 |
| 713.48 | 7.28 | 7.28 |
| 727.51 | 7.67 | 7.67 |
| 705.56 | 7.81 | 7.81 |
| 658.46 | 7.60 | 7.60 |
| 638.93 | 7.68 | 7.68 |
| 650.97 | 7.36 | 7.36 |
| 662.98 | 7.50 | 7.50 |
| 620.45 | 7.40 | 7.40 |