Basic Information
ID: ALA3705261
Type: Binding
Description: Luciferase-Based Assay: Recombinant human c-Src or Yes (28 ng/well, Panvera/Invitrogen, Madison Wis.), ATP (3 μM), a tyrosine kinase substrate (PTK2, 250 μM, Promega Corp., Madison Wis.), and test agents (at concentrations ranging from about 1 nM/l to about 100 μM/l), in the presence of Src kinase reaction buffer (Upstate USA, Lake Placid N.Y.). After reacting for about 90 minutes at room temperature, residual ATP was determined using a luciferase-based assay (KinaseGlo, Promega Corp.) as a measure of kinase activity.
Format: BAO_0000357
Target: Tyrosine-protein kinase SRC(ALA267)
Document: ALA3639196
BindingDB Source: 5868
Activity Charts
Bioactivity
Activity Types for Assay ALA3705261
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 354.46 | 5.67 | 5.67 |
| 368.48 | 5.98 | 5.98 |
| 368.48 | 5.98 | 5.98 |
| 341.42 | 4.76 | 4.76 |
| 306.37 | 3.58 | 3.58 |
| 455.61 | 6.08 | 6.08 |
| 453.59 | 5.84 | 5.84 |
| 502.64 | 4.30 | 4.30 |
| 502.64 | 4.30 | 4.30 |
| 383.50 | 5.43 | 5.43 |
| 370.46 | 5.37 | 5.37 |
| 494.43 | 6.53 | 6.53 |
| 459.98 | 5.87 | 5.87 |
| 461.52 | 5.50 | 5.50 |
| 444.54 | 4.82 | 4.82 |
| 426.52 | 4.61 | 4.61 |
| 480.40 | 6.22 | 6.22 |
| 516.67 | 4.73 | 4.73 |
| 557.51 | 5.42 | 5.42 |
| 518.64 | 3.61 | 3.61 |
| 454.58 | 5.11 | 5.11 |
| 468.61 | 5.57 | 5.57 |
| 496.62 | 5.49 | 5.49 |
| 532.67 | 4.90 | 4.90 |
| 441.90 | 3.75 | 3.75 |
| 553.09 | 4.82 | 4.82 |
| 567.12 | 5.21 | 5.21 |
| 581.14 | 5.60 | 5.60 |
| 569.09 | 4.06 | 4.06 |
| 538.07 | 5.36 | 5.36 |
| 573.51 | 5.13 | 5.13 |
| 540.60 | 4.10 | 4.10 |
| 518.02 | 5.80 | 5.80 |
| 360.39 | 4.89 | 4.89 |
| 409.54 | 5.35 | 5.35 |
| 391.48 | 5.91 | 5.91 |
| 346.82 | 5.40 | 5.40 |
| 370.46 | 5.37 | 5.37 |
| 386.46 | 5.07 | 5.07 |
| 400.48 | 5.38 | 5.38 |
| 416.48 | 5.16 | 5.16 |
| 400.48 | 5.38 | 5.38 |
| 402.45 | 4.77 | 4.77 |
| 402.45 | 4.77 | 4.77 |
| 411.29 | 6.06 | 6.06 |
| 382.25 | 5.45 | 5.45 |
| 382.25 | 5.45 | 5.45 |
| 427.51 | 4.01 | 4.01 |
| 469.59 | 5.54 | 5.54 |
| 496.66 | 5.38 | 5.38 |
| 496.66 | 5.38 | 5.38 |
| 361.41 | 4.36 | 4.36 |
| 457.54 | 3.74 | 3.74 |
| 523.47 | 5.68 | 5.68 |
| 537.50 | 6.07 | 6.07 |
| 480.62 | 5.19 | 5.19 |
| 454.58 | 4.65 | 4.65 |
| 480.62 | 5.19 | 5.19 |
| 516.67 | 4.73 | 4.73 |
| 456.55 | 4.34 | 4.34 |
| 375.86 | 5.41 | 5.41 |
| 519.44 | 6.40 | 6.40 |
| 468.61 | 5.04 | 5.04 |
| 530.70 | 5.12 | 5.12 |
| 530.70 | 5.08 | 5.08 |
| 500.56 | 4.67 | 4.67 |
| 545.67 | 4.08 | 4.08 |
| 503.01 | 4.79 | 4.79 |
| 453.55 | 3.83 | 3.83 |
| 488.98 | 4.49 | 4.49 |
| 518.64 | 4.13 | 4.13 |
| 454.58 | 4.70 | 4.70 |
| 441.54 | 4.92 | 4.92 |
| 518.64 | 3.61 | 3.61 |
| 559.70 | 4.38 | 4.38 |
| 601.73 | 4.64 | 4.64 |
| 383.88 | 3.87 | 3.87 |
| 602.77 | 5.04 | 5.04 |
| 476.97 | 4.39 | 4.39 |
| 381.80 | 4.64 | 4.64 |
| 474.54 | 4.61 | 4.61 |
| 511.01 | 3.70 | 3.70 |
| 522.61 | 3.96 | 3.96 |
| 457.55 | 5.67 | 5.67 |
| 411.94 | 4.65 | 4.65 |
| 511.65 | 2.41 | 2.41 |
| 554.08 | 4.55 | 4.55 |
| 475.98 | 5.58 | 5.58 |
| 493.40 | 5.10 | 5.10 |
| 571.53 | 5.77 | 5.77 |
| 491.98 | 4.68 | 4.68 |
| 492.05 | 6.29 | 6.29 |
| 522.61 | 3.96 | 3.96 |
| 522.61 | 3.96 | 3.96 |
| 522.61 | 3.96 | 3.96 |
| 556.06 | 5.50 | 5.50 |
| 518.64 | 4.13 | 4.13 |
| 643.17 | 5.99 | 5.99 |
| 571.08 | 4.96 | 4.96 |
| 535.02 | 5.41 | 5.41 |