Basic Information
ID: ALA3705261
Type: Binding
Description: Luciferase-Based Assay: Recombinant human c-Src or Yes (28 ng/well, Panvera/Invitrogen, Madison Wis.), ATP (3 μM), a tyrosine kinase substrate (PTK2, 250 μM, Promega Corp., Madison Wis.), and test agents (at concentrations ranging from about 1 nM/l to about 100 μM/l), in the presence of Src kinase reaction buffer (Upstate USA, Lake Placid N.Y.). After reacting for about 90 minutes at room temperature, residual ATP was determined using a luciferase-based assay (KinaseGlo, Promega Corp.) as a measure of kinase activity.
Activity Charts
Bioactivity
Activity Types for Assay ALA3705261
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 340.43 | 5.36 | 5.36 |
| 354.46 | 5.67 | 5.67 |
| 354.46 | 5.67 | 5.67 |
| 391.48 | 5.91 | 5.91 |
| 326.40 | 5.05 | 5.05 |
| 396.54 | 6.70 | 6.70 |
| 374.88 | 6.01 | 6.01 |
| 368.48 | 5.98 | 5.98 |
| 488.62 | 3.95 | 3.95 |
| 488.62 | 3.95 | 3.95 |
| 368.48 | 5.98 | 5.98 |
| 368.48 | 5.98 | 5.98 |
| 419.51 | 4.01 | 4.01 |
| 341.42 | 4.76 | 4.76 |
| 341.42 | 4.76 | 4.76 |
| 306.37 | 3.58 | 3.58 |
| 368.44 | 4.87 | 4.87 |
| 455.61 | 6.08 | 6.08 |
| 455.61 | 6.08 | 6.08 |
| 453.59 | 5.84 | 5.84 |
| 453.59 | 5.84 | 5.84 |
| 502.64 | 4.30 | 4.30 |
| 502.64 | 4.30 | 4.30 |
| 383.50 | 5.43 | 5.43 |
| 370.46 | 5.37 | 5.37 |
| 370.46 | 5.37 | 5.37 |
| 384.48 | 5.76 | 5.76 |
| 354.46 | 5.67 | 5.67 |
| 368.48 | 5.98 | 5.98 |
| 425.54 | 5.22 | 5.22 |
| 494.43 | 6.53 | 6.53 |
| 459.98 | 5.87 | 5.87 |
| 461.52 | 5.50 | 5.50 |
| 490.01 | 5.88 | 5.88 |
| 490.01 | 5.88 | 5.88 |
| 490.01 | 5.88 | 5.88 |
| 444.54 | 4.82 | 4.82 |
| 426.52 | 4.61 | 4.61 |
| 439.56 | 5.53 | 5.53 |
| 480.40 | 6.22 | 6.22 |
| 453.59 | 5.84 | 5.84 |
| 516.67 | 4.73 | 4.73 |
| 557.51 | 5.42 | 5.42 |
| 539.06 | 4.48 | 4.48 |
| 527.65 | 4.30 | 4.30 |
| 538.08 | 4.35 | 4.35 |
| 503.63 | 3.70 | 3.70 |
| 517.66 | 4.16 | 4.16 |
| 581.72 | 3.49 | 3.49 |
| 516.67 | 4.73 | 4.73 |
| 488.62 | 4.12 | 4.12 |
| 491.42 | 3.21 | 3.21 |
| 502.64 | 4.43 | 4.43 |
| 556.61 | 5.14 | 5.14 |
| 527.65 | 4.30 | 4.30 |
| 520.63 | 4.57 | 4.57 |
| 518.64 | 3.61 | 3.61 |
| 467.62 | 6.23 | 6.23 |
| 482.63 | 4.99 | 4.99 |
| 482.63 | 4.99 | 4.99 |
| 454.58 | 5.23 | 5.23 |
| 454.58 | 5.11 | 5.11 |
| 468.61 | 5.57 | 5.57 |
| 496.62 | 5.49 | 5.49 |
| 532.67 | 4.90 | 4.90 |
| 475.98 | 5.58 | 5.58 |
| 474.95 | 4.15 | 4.15 |
| 488.98 | 4.49 | 4.49 |
| 524.00 | 3.97 | 3.97 |
| 441.90 | 3.75 | 3.75 |
| 539.06 | 4.48 | 4.48 |
| 553.09 | 4.82 | 4.82 |
| 567.12 | 5.21 | 5.21 |
| 581.14 | 5.60 | 5.60 |
| 569.09 | 4.06 | 4.06 |
| 511.01 | 3.70 | 3.70 |
| 538.07 | 5.36 | 5.36 |
| 510.02 | 4.63 | 4.63 |
| 573.51 | 5.13 | 5.13 |
| 557.05 | 4.62 | 4.62 |
| 540.60 | 4.10 | 4.10 |
| 583.51 | 4.59 | 4.59 |
| 560.10 | 6.82 | 6.82 |
| 580.09 | 7.09 | 7.09 |
| 594.12 | 7.40 | 7.40 |
| 546.07 | 6.43 | 6.43 |
| 518.02 | 5.80 | 5.80 |
| 376.51 | 2.97 | 2.97 |
| 360.39 | 4.89 | 4.89 |
| 372.43 | 4.76 | 4.76 |
| 404.56 | 3.67 | 3.67 |
| 405.51 | 5.92 | 5.92 |
| 409.54 | 5.35 | 5.35 |
| 391.48 | 5.91 | 5.91 |
| 354.46 | 5.67 | 5.67 |
| 392.87 | 6.15 | 6.15 |
| 346.82 | 5.40 | 5.40 |
| 372.43 | 4.76 | 4.76 |
| 370.46 | 5.37 | 5.37 |
| 409.32 | 6.67 | 6.67 |
| 386.46 | 5.07 | 5.07 |
| 386.46 | 5.07 | 5.07 |
| 400.48 | 5.38 | 5.38 |
| 400.48 | 5.38 | 5.38 |
| 400.48 | 5.38 | 5.38 |
| 428.54 | 6.10 | 6.10 |
| 416.48 | 5.16 | 5.16 |
| 373.42 | 4.16 | 4.16 |
| 400.48 | 5.38 | 5.38 |
| 402.45 | 4.77 | 4.77 |
| 402.45 | 4.77 | 4.77 |
| 411.29 | 6.06 | 6.06 |
| 411.29 | 6.06 | 6.06 |
| 382.25 | 5.45 | 5.45 |
| 382.25 | 5.45 | 5.45 |
| 469.59 | 5.07 | 5.07 |
| 427.51 | 4.01 | 4.01 |
| 371.44 | 4.76 | 4.76 |
| 469.59 | 5.54 | 5.54 |
| 496.66 | 5.38 | 5.38 |
| 496.66 | 5.38 | 5.38 |
| 361.41 | 4.36 | 4.36 |
| 466.59 | 4.75 | 4.75 |
| 457.54 | 3.74 | 3.74 |
| 473.58 | 3.97 | 3.97 |
| 488.62 | 4.12 | 4.12 |
| 523.47 | 5.68 | 5.68 |
| 523.47 | 5.68 | 5.68 |
| 537.50 | 6.07 | 6.07 |
| 537.50 | 6.07 | 6.07 |
| 482.59 | 3.99 | 3.99 |
| 480.62 | 5.19 | 5.19 |
| 388.43 | 4.74 | 4.74 |
| 454.58 | 4.65 | 4.65 |
| 480.62 | 5.19 | 5.19 |
| 374.40 | 4.65 | 4.65 |
| 519.44 | 6.40 | 6.40 |
| 516.67 | 4.73 | 4.73 |
| 456.55 | 4.34 | 4.34 |
| 375.86 | 5.41 | 5.41 |
| 375.86 | 5.41 | 5.41 |
| 470.58 | 4.78 | 4.78 |
| 490.63 | 4.20 | 4.20 |
| 490.63 | 4.20 | 4.20 |
| 487.63 | 4.64 | 4.64 |
| 495.41 | 5.92 | 5.92 |
| 519.44 | 6.40 | 6.40 |
| 452.56 | 4.45 | 4.45 |
| 523.69 | 4.73 | 4.73 |
| 468.61 | 5.04 | 5.04 |
| 530.70 | 5.12 | 5.12 |
| 484.60 | 5.17 | 5.17 |
| 487.63 | 4.64 | 4.64 |
| 588.73 | 5.60 | 5.60 |
| 530.70 | 5.08 | 5.08 |
| 452.56 | 4.41 | 4.41 |
| 531.69 | 3.54 | 3.54 |
| 466.59 | 4.75 | 4.75 |
| 495.41 | 5.92 | 5.92 |
| 515.68 | 5.62 | 5.62 |
| 500.56 | 4.67 | 4.67 |
| 545.67 | 4.08 | 4.08 |
| 503.01 | 4.79 | 4.79 |
| 473.97 | 4.18 | 4.18 |
| 453.55 | 3.83 | 3.83 |
| 440.55 | 4.92 | 4.92 |
| 487.01 | 5.09 | 5.09 |
| 581.72 | 3.49 | 3.49 |
| 503.63 | 3.70 | 3.70 |
| 445.59 | 5.59 | 5.59 |
| 488.98 | 4.49 | 4.49 |
| 518.64 | 4.13 | 4.13 |
| 457.55 | 5.67 | 5.67 |
| 454.58 | 4.70 | 4.70 |
| 527.65 | 4.60 | 4.60 |
| 320.40 | 3.46 | 3.46 |
| 557.51 | 5.42 | 5.42 |
| 441.54 | 4.92 | 4.92 |
| 518.64 | 3.61 | 3.61 |
| 363.81 | 4.50 | 4.50 |
| 559.70 | 4.38 | 4.38 |
| 601.73 | 4.64 | 4.64 |
| 545.67 | 4.08 | 4.08 |
| 529.09 | 4.83 | 4.83 |
| 383.88 | 3.87 | 3.87 |
| 492.97 | 4.52 | 4.52 |
| 602.77 | 5.04 | 5.04 |
| 518.64 | 4.13 | 4.13 |
| 476.97 | 4.39 | 4.39 |
| 524.67 | 3.67 | 3.67 |
| 504.62 | 3.82 | 3.82 |
| 510.02 | 4.38 | 4.38 |
| 513.60 | 4.43 | 4.43 |
| 381.80 | 4.64 | 4.64 |
| 474.54 | 4.61 | 4.61 |
| 347.35 | 3.98 | 3.98 |
| 405.89 | 5.17 | 5.17 |
| 420.86 | 4.46 | 4.46 |
| 393.83 | 4.51 | 4.51 |
| 511.01 | 3.70 | 3.70 |
| 459.53 | 5.06 | 5.06 |
| 522.61 | 3.96 | 3.96 |
| 532.67 | 4.52 | 4.52 |
| 457.55 | 5.67 | 5.67 |
| 478.58 | 2.84 | 2.84 |
| 411.94 | 4.65 | 4.65 |
| 539.06 | 4.48 | 4.48 |
| 462.94 | 4.66 | 4.66 |
| 548.67 | 3.49 | 3.49 |
| 448.91 | 4.33 | 4.33 |
| 475.98 | 5.58 | 5.58 |
| 557.72 | 2.67 | 2.67 |
| 513.67 | 2.66 | 2.66 |
| 513.02 | 3.94 | 3.94 |
| 511.65 | 2.41 | 2.41 |
| 447.93 | 3.91 | 3.91 |
| 554.08 | 4.55 | 4.55 |
| 475.98 | 5.58 | 5.58 |
| 503.99 | 4.32 | 4.32 |
| 519.99 | 3.25 | 3.25 |
| 491.98 | 3.61 | 3.61 |
| 525.03 | 4.04 | 4.04 |
| 496.40 | 5.92 | 5.92 |
| 482.59 | 5.18 | 5.18 |
| 529.45 | 4.64 | 4.64 |
| 599.59 | 6.55 | 6.55 |
| 493.40 | 5.10 | 5.10 |
| 531.64 | 3.22 | 3.22 |
| 571.53 | 5.77 | 5.77 |
| 397.27 | 5.76 | 5.76 |
| 525.44 | 4.26 | 4.26 |
| 507.00 | 3.32 | 3.32 |
| 510.43 | 5.62 | 5.62 |
| 525.03 | 4.04 | 4.04 |
| 556.52 | 6.31 | 6.31 |
| 491.98 | 4.68 | 4.68 |
| 492.05 | 6.29 | 6.29 |
| 505.07 | 5.88 | 5.88 |
| 492.05 | 6.29 | 6.29 |
| 478.00 | 5.31 | 5.31 |
| 522.61 | 3.96 | 3.96 |
| 522.61 | 3.96 | 3.96 |
| 522.61 | 3.96 | 3.96 |
| 553.09 | 4.82 | 4.82 |
| 517.03 | 5.27 | 5.27 |
| 510.43 | 6.23 | 6.23 |
| 556.06 | 5.50 | 5.50 |
| 544.46 | 5.28 | 5.28 |
| 518.64 | 4.13 | 4.13 |
| 643.17 | 5.99 | 5.99 |
| 572.52 | 6.02 | 6.02 |
| 539.61 | 4.99 | 4.99 |
| 571.08 | 4.96 | 4.96 |
| 535.02 | 5.41 | 5.41 |
| 510.43 | 5.76 | 5.76 |
| 518.02 | 5.80 | 5.80 |
| 587.53 | 5.47 | 5.47 |
| 520.62 | 3.52 | 3.52 |