Basic Information
ID: ALA3705358
Type: Binding
Description: Inhibition Assay: Experimental was performed by measuring the formation of NADH at 340 nm with a fluorescence spectrophotometer. Specifically, 2 ml (in total) of the solution containing 50 mM Tris-HCl (pH 7.5), 0.1 mM DTT, 0.25 mM NAD+, 10 μg of purified 15-PGDH enzyme, 21 μM PGE2 and various concentrations (0.0001 μM to 64 μM) of the derivatives according to the present invention was added to each cell. The absorbance of the reaction mixture was recorded at 340 nm so that the activities of the derivatives according to the present invention as 15-PGDH inhibitors were determined from a standard curve prepared from the absorbance of various concentrations of NADH at 340 nm.
Organism: Homo sapiens
Activity Charts
Bioactivity
Activity Types for Assay ALA3705358
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 311.36 | 3.79 | 3.79 |
| 303.38 | 3.78 | 3.78 |
| 221.24 | 1.72 | 1.72 |
| 319.38 | 3.16 | 3.16 |
| 317.41 | 4.17 | 4.17 |
| 369.40 | 3.43 | 3.43 |
| 331.44 | 4.56 | 4.56 |
| 219.26 | 2.52 | 2.52 |
| 331.44 | 4.56 | 4.56 |
| 382.46 | 1.32 | 1.32 |
| 331.42 | 3.90 | 3.90 |
| 334.40 | 1.93 | 1.93 |
| 331.42 | 3.90 | 3.90 |
| 307.37 | 3.02 | 3.02 |
| 326.38 | 3.23 | 3.23 |
| 346.45 | 3.52 | 3.52 |
| 332.43 | 3.08 | 3.08 |
| 346.43 | 3.60 | 3.60 |
| 396.49 | 1.71 | 1.71 |
| 317.39 | 3.86 | 3.86 |
| 317.39 | 3.86 | 3.86 |
| 317.41 | 4.17 | 4.17 |
| 301.32 | 3.39 | 3.39 |
| 312.35 | 3.19 | 3.19 |
| 325.39 | 4.10 | 4.10 |
| 355.37 | 3.52 | 3.52 |
| 359.83 | 4.53 | 4.53 |
| 331.44 | 4.42 | 4.42 |
| 347.44 | 3.94 | 3.94 |
| 375.49 | 4.06 | 4.06 |
| 297.33 | 4.01 | 4.01 |
| 365.88 | 5.22 | 5.22 |
| 410.33 | 5.33 | 5.33 |
| 337.83 | 4.58 | 4.58 |
| 351.86 | 4.83 | 4.83 |
| 379.91 | 5.61 | 5.61 |
| 393.94 | 6.00 | 6.00 |
| 416.91 | 1.98 | 1.98 |
| 365.86 | 4.55 | 4.55 |
| 341.82 | 3.67 | 3.67 |
| 351.86 | 4.83 | 4.83 |
| 345.81 | 4.45 | 4.45 |
| 359.83 | 4.49 | 4.49 |
| 373.86 | 4.88 | 4.88 |
| 387.89 | 5.27 | 5.27 |
| 345.81 | 4.45 | 4.45 |
| 359.83 | 4.49 | 4.49 |
| 373.86 | 4.88 | 4.88 |
| 387.89 | 5.27 | 5.27 |
| 365.88 | 5.22 | 5.22 |
| 360.82 | 3.88 | 3.88 |
| 390.46 | 4.86 | 4.86 |
| 347.51 | 5.36 | 5.36 |
| 399.43 | 5.58 | 5.58 |
| 349.43 | 4.70 | 4.70 |
| 303.38 | 3.93 | 3.93 |
| 345.46 | 4.95 | 4.95 |
| 359.49 | 5.34 | 5.34 |
| 376.43 | 4.47 | 4.47 |
| 325.39 | 3.84 | 3.84 |
| 339.42 | 4.23 | 4.23 |
| 353.44 | 4.62 | 4.62 |
| 361.46 | 4.57 | 4.57 |
| 361.46 | 4.06 | 4.06 |
| 387.46 | 5.46 | 5.46 |
| 383.87 | 5.36 | 5.36 |
| 395.91 | 5.23 | 5.23 |
| 399.43 | 5.58 | 5.58 |
| 404.49 | 5.25 | 5.25 |
| 376.43 | 4.47 | 4.47 |
| 427.46 | 1.23 | 1.23 |
| 365.88 | 5.07 | 5.07 |
| 341.82 | 3.67 | 3.67 |
| 337.83 | 4.58 | 4.58 |
| 351.86 | 4.83 | 4.83 |
| 379.91 | 5.61 | 5.61 |
| 393.94 | 6.00 | 6.00 |
| 361.44 | 3.70 | 3.70 |
| 380.88 | 4.25 | 4.25 |
| 380.88 | 4.25 | 4.25 |
| 361.42 | 3.33 | 3.33 |
| 333.45 | 4.08 | 4.08 |
| 319.43 | 3.69 | 3.69 |
| 305.40 | 3.30 | 3.30 |
| 353.87 | 4.35 | 4.35 |
| 319.43 | 3.69 | 3.69 |
| 384.48 | 0.84 | 0.84 |
| 367.90 | 4.74 | 4.74 |
| 347.48 | 4.39 | 4.39 |
| 381.92 | 5.13 | 5.13 |
| 367.90 | 4.74 | 4.74 |
| 361.51 | 4.78 | 4.78 |
| 389.48 | 4.98 | 4.98 |
| 333.45 | 4.08 | 4.08 |
| 313.38 | 3.31 | 3.31 |
| 331.44 | 4.48 | 4.48 |
| 390.80 | 4.36 | 4.36 |
| 361.44 | 3.91 | 3.91 |
| 375.49 | 4.96 | 4.96 |
| 399.43 | 5.44 | 5.44 |
| 345.81 | 4.45 | 4.45 |
| 345.46 | 4.87 | 4.87 |
| 375.45 | 2.96 | 2.96 |
| 373.86 | 4.88 | 4.88 |
| 351.86 | 4.83 | 4.83 |
| 346.45 | 4.15 | 4.15 |
| 361.46 | 4.06 | 4.06 |
| 379.91 | 5.61 | 5.61 |
| 391.49 | 4.07 | 4.07 |
| 337.83 | 4.58 | 4.58 |
| 387.89 | 5.27 | 5.27 |
| 376.43 | 4.47 | 4.47 |
| 359.49 | 5.26 | 5.26 |
| 381.88 | 4.60 | 4.60 |
| 360.82 | 3.88 | 3.88 |
| 375.49 | 4.96 | 4.96 |
| 235.26 | 1.71 | 1.71 |
| 373.52 | 5.65 | 5.65 |
| 395.91 | 5.23 | 5.23 |
| 365.88 | 5.22 | 5.22 |
| 357.48 | 5.12 | 5.12 |
| 359.83 | 4.49 | 4.49 |
| 393.94 | 6.00 | 6.00 |
| 351.86 | 4.83 | 4.83 |