Basic Information
ID: ALA3705369
Type: Binding
Description: Inhibition Assay: Using a 96 well plate (#3915, Costar), a test compound (25 μL) was mixed with 20 μM fluorescence enzyme substrate (Boc-Phe-Ser-Arg-AMC, 50 μL) mixed with 200 mM Tris-HCl buffer (pH 8.0), and human trypsin (Sigma, 25 μL) was added. Using a fluorescence plate reader fmax (Molecular Devices, Inc.), the reaction rate was measured from the time-course changes at excitation wavelength 355 nm and fluorescence wavelength 460 nm.
Organism: Homo sapiens
Activity Charts
Bioactivity
Activity Types for Assay ALA3705369
Ki
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 491.50 | 2.15 | 2.15 |
| 505.52 | 2.54 | 2.54 |
| 421.45 | 2.16 | 2.16 |
| 451.48 | 1.52 | 1.52 |
| 461.52 | 3.06 | 3.06 |
| 449.50 | 2.94 | 2.94 |
| 435.48 | 2.50 | 2.50 |
| 461.52 | 3.03 | 3.03 |
| 463.53 | 3.18 | 3.18 |
| 463.53 | 3.28 | 3.28 |
| 479.53 | 2.30 | 2.30 |
| 491.59 | 3.96 | 3.96 |
| 463.53 | 3.33 | 3.33 |
| 493.56 | 2.69 | 2.69 |
| 539.63 | 4.55 | 4.55 |
| 489.57 | 3.81 | 3.81 |
| 392.45 | 3.82 | 3.82 |
| 507.54 | 2.78 | 2.78 |
| 491.59 | 4.06 | 4.06 |
| 463.53 | 3.28 | 3.28 |
| 511.60 | 2.86 | 2.86 |
| 521.57 | 3.17 | 3.17 |
| 475.54 | 3.28 | 3.28 |
| 463.53 | 3.33 | 3.33 |
| 477.56 | 3.72 | 3.72 |
| 491.59 | 4.11 | 4.11 |
| 477.56 | 3.72 | 3.72 |
| 483.52 | 4.30 | 4.30 |
| 483.52 | 4.30 | 4.30 |
| 475.54 | 3.17 | 3.17 |
| 503.60 | 4.11 | 4.11 |
| 497.55 | 3.90 | 3.90 |
| 440.50 | 3.99 | 3.99 |
| 456.50 | 3.29 | 3.29 |
| 475.54 | 3.42 | 3.42 |
| 520.58 | 2.57 | 2.57 |
| 506.56 | 2.18 | 2.18 |
| 470.53 | 4.00 | 4.00 |
| 439.46 | 3.44 | 3.44 |
| 499.52 | 4.00 | 4.00 |
| 525.60 | 5.01 | 5.01 |
| 523.59 | 4.24 | 4.24 |
| 470.53 | 4.00 | 4.00 |
| 506.60 | 3.05 | 3.05 |
| 525.60 | 4.75 | 4.75 |
| 458.49 | 4.13 | 4.13 |
| 461.52 | 3.08 | 3.08 |
| 447.49 | 2.69 | 2.69 |
| 593.63 | 3.41 | 3.41 |
| 527.53 | 3.99 | 3.99 |
| 555.58 | 4.17 | 4.17 |
| 433.46 | 2.25 | 2.25 |
| 491.59 | 4.11 | 4.11 |
| 416.48 | 2.99 | 2.99 |
| 459.51 | 1.83 | 1.83 |
| 445.48 | 1.79 | 1.79 |
| 555.58 | 4.77 | 4.77 |
| 569.61 | 4.86 | 4.86 |
| 527.53 | 3.99 | 3.99 |
| 489.57 | 3.72 | 3.72 |
| 505.61 | 4.50 | 4.50 |
| 447.49 | 2.50 | 2.50 |
| 483.52 | 4.30 | 4.30 |
| 527.53 | 3.99 | 3.99 |
| 475.54 | 3.28 | 3.28 |
| 506.56 | 2.06 | 2.06 |
| 489.57 | 3.72 | 3.72 |
| 475.54 | 3.47 | 3.47 |
| 489.57 | 3.86 | 3.86 |
| 473.53 | 2.57 | 2.57 |
| 513.55 | 3.61 | 3.61 |
| 461.52 | 2.89 | 2.89 |
| 519.64 | 4.74 | 4.74 |