Basic Information
ID: ALA3705378
Type: Binding
Description: ELISA Assay: HEK293 cells are seeded in 96-well poly-lysine coated plates at 20,000 cells per well in DMEM (10% FBS, 1% NEAA, 0.1% glutamine). Following overnight incubation, the cells are washed with 100 μL of Opti-MEM (Gibco, Carlsbad, Calif.) to remove serum. Compound in DMSO is serially diluted (beginning with 100 μM) in Opti-MEM and added to the cells. The conditioned media is analyzed for VEGF with a Quantikine human VEGF immunoassay kit (R&D Systems, Minneapolis, Minn.). Optical density measurements at 450 nm are recorded using the Spectra Max 250 (Molecular Devices, Sunnyvale, Calif.).
Organism: Homo sapiens
Cell type: HEK293
Activity Charts
Bioactivity
Activity Types for Assay ALA3705378
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 433.94 | 3.31 | 3.31 |
| 326.44 | 3.27 | 3.27 |
| 338.41 | 1.98 | 1.98 |
| 334.42 | 3.45 | 3.45 |
| 304.42 | 2.05 | 2.05 |
| 411.89 | 2.37 | 2.37 |
| 341.41 | 1.36 | 1.36 |
| 389.50 | 2.92 | 2.92 |
| 342.44 | 2.47 | 2.47 |
| 445.59 | 2.55 | 2.55 |
| 475.96 | 1.86 | 1.86 |
| 354.84 | 3.55 | 3.55 |
| 366.40 | 0.62 | 0.62 |
| 235.67 | 2.26 | 2.26 |
| 235.67 | 2.26 | 2.26 |
| 328.41 | 1.95 | 1.95 |