Basic Information
ID: ALA3705381
Type: Binding
Description: IMAP Assay: Cdk9/cyclinT1 is purchased from Millipore, cat #14-685. The final total protein concentration in the assay 4 nM. The 5TAMRA-cdk7tide peptide substrate, 5TAMRA-YSPTSPSYSPTSPSYSTPSPS--COOH, is purchased from Molecular Devices, cat#R7352. The final concentration of peptide substrate is 100 nM. The ATP substrate (Adenosine-5'-triphosphate) is purchased from Roche Diagnostics, cat#1140965. The final concentration of ATP substrate is 6 uM. IMAP (Immobilized Metal Assay for Phosphochemicals) Progressive Binding reagent is purchased from Molecular Devices, cat#R8139. Fluorescence polarization (FP) is used for detection. The 5TAMRA-cdk7tide peptide is phosphorylated by Cdk9/cyclinT1 kinase using the ATP substrate. The Phospho-5TAMRA-cdk7tide peptide substrate is bound to the IMAP Progressive Binding Reagent.
Organism: Homo sapiens
Activity Charts
Bioactivity
Activity Types for Assay ALA3705381
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 439.97 | 5.48 | 5.48 |
| 411.91 | 4.71 | 4.71 |
| 504.03 | 4.77 | 4.77 |
| 511.05 | 4.95 | 4.95 |
| 401.94 | 3.68 | 3.68 |
| 440.95 | 5.44 | 5.44 |
| 482.61 | 5.80 | 5.80 |
| 459.49 | 5.60 | 5.60 |
| 443.93 | 5.37 | 5.37 |
| 440.95 | 4.81 | 4.81 |
| 468.96 | 4.33 | 4.33 |
| 450.95 | 5.10 | 5.10 |
| 468.96 | 4.33 | 4.33 |
| 469.95 | 4.93 | 4.93 |
| 453.99 | 5.83 | 5.83 |
| 469.99 | 4.85 | 4.85 |
| 532.09 | 4.91 | 4.91 |
| 439.97 | 5.49 | 5.49 |
| 453.99 | 5.74 | 5.74 |
| 498.02 | 6.51 | 6.51 |
| 414.00 | 5.47 | 5.47 |
| 407.95 | 5.09 | 5.09 |
| 408.94 | 4.49 | 4.49 |
| 443.93 | 5.37 | 5.37 |
| 408.94 | 4.49 | 4.49 |
| 443.93 | 5.37 | 5.37 |
| 392.48 | 3.97 | 3.97 |
| 391.49 | 4.58 | 4.58 |
| 374.49 | 3.83 | 3.83 |
| 426.93 | 4.63 | 4.63 |
| 409.93 | 3.88 | 3.88 |
| 473.96 | 5.69 | 5.69 |
| 442.39 | 5.74 | 5.74 |
| 426.93 | 4.63 | 4.63 |
| 443.93 | 5.37 | 5.37 |
| 415.97 | 4.32 | 4.32 |
| 425.94 | 5.23 | 5.23 |
| 442.39 | 5.74 | 5.74 |
| 421.98 | 5.40 | 5.40 |
| 437.98 | 5.10 | 5.10 |
| 491.95 | 5.99 | 5.99 |
| 487.83 | 5.25 | 5.25 |
| 475.95 | 6.11 | 6.11 |
| 423.95 | 4.80 | 4.80 |
| 425.94 | 5.23 | 5.23 |
| 421.98 | 5.40 | 5.40 |
| 475.95 | 6.11 | 6.11 |
| 473.96 | 5.69 | 5.69 |
| 491.95 | 5.99 | 5.99 |
| 442.39 | 5.74 | 5.74 |
| 428.02 | 5.72 | 5.72 |
| 421.98 | 5.34 | 5.34 |
| 422.96 | 4.73 | 4.73 |
| 440.95 | 4.87 | 4.87 |
| 422.96 | 4.73 | 4.73 |
| 457.96 | 5.62 | 5.62 |
| 456.42 | 5.99 | 5.99 |
| 457.96 | 5.62 | 5.62 |
| 487.98 | 5.94 | 5.94 |
| 457.96 | 5.62 | 5.62 |
| 439.97 | 5.48 | 5.48 |
| 512.03 | 6.36 | 6.36 |
| 399.97 | 4.95 | 4.95 |
| 393.92 | 4.57 | 4.57 |
| 412.90 | 4.11 | 4.11 |
| 429.90 | 4.85 | 4.85 |
| 394.91 | 3.97 | 3.97 |
| 428.37 | 5.23 | 5.23 |
| 429.90 | 4.85 | 4.85 |
| 428.02 | 5.86 | 5.86 |
| 421.98 | 5.12 | 5.12 |
| 439.97 | 5.25 | 5.25 |
| 518.06 | 5.21 | 5.21 |
| 532.09 | 5.46 | 5.46 |
| 537.08 | 5.48 | 5.48 |
| 531.04 | 6.10 | 6.10 |
| 525.07 | 5.20 | 5.20 |
| 551.11 | 5.73 | 5.73 |
| 545.06 | 6.34 | 6.34 |
| 497.02 | 4.51 | 4.51 |
| 523.06 | 5.05 | 5.05 |
| 517.01 | 5.66 | 5.66 |
| 453.99 | 5.88 | 5.88 |
| 399.97 | 4.95 | 4.95 |
| 401.94 | 3.80 | 3.80 |
| 411.91 | 4.71 | 4.71 |
| 429.90 | 4.85 | 4.85 |
| 394.91 | 3.97 | 3.97 |
| 429.90 | 4.85 | 4.85 |
| 399.97 | 4.95 | 4.95 |
| 401.94 | 3.80 | 3.80 |
| 411.91 | 4.71 | 4.71 |
| 429.90 | 4.85 | 4.85 |
| 394.91 | 3.97 | 3.97 |
| 429.90 | 4.85 | 4.85 |
| 426.93 | 4.63 | 4.63 |
| 468.02 | 6.08 | 6.08 |
| 425.94 | 5.05 | 5.05 |
| 401.94 | 4.07 | 4.07 |
| 436.99 | 4.90 | 4.90 |
| 401.94 | 4.07 | 4.07 |
| 415.97 | 4.32 | 4.32 |
| 462.04 | 5.86 | 5.86 |
| 417.94 | 3.31 | 3.31 |
| 426.92 | 5.20 | 5.20 |
| 430.00 | 4.34 | 4.34 |
| 424.94 | 3.46 | 3.46 |
| 508.09 | 7.00 | 7.00 |
| 523.10 | 5.77 | 5.77 |
| 494.06 | 6.61 | 6.61 |
| 467.98 | 5.26 | 5.26 |
| 426.92 | 5.26 | 5.26 |
| 494.06 | 6.76 | 6.76 |
| 496.03 | 5.60 | 5.60 |
| 361.88 | 2.88 | 2.88 |
| 375.90 | 3.27 | 3.27 |
| 415.97 | 3.92 | 3.92 |
| 409.92 | 4.52 | 4.52 |
| 415.97 | 3.92 | 3.92 |
| 409.92 | 4.52 | 4.52 |
| 415.97 | 3.92 | 3.92 |
| 403.91 | 3.06 | 3.06 |
| 402.93 | 4.10 | 4.10 |
| 416.95 | 4.35 | 4.35 |
| 402.93 | 4.10 | 4.10 |
| 416.95 | 4.35 | 4.35 |
| 402.93 | 4.10 | 4.10 |
| 402.93 | 3.47 | 3.47 |
| 388.90 | 3.35 | 3.35 |
| 414.98 | 5.50 | 5.50 |
| 444.91 | 5.40 | 5.40 |
| 426.92 | 5.26 | 5.26 |
| 418.93 | 3.34 | 3.34 |
| 415.97 | 3.69 | 3.69 |
| 425.94 | 5.23 | 5.23 |
| 396.90 | 5.90 | 5.90 |
| 410.92 | 6.29 | 6.29 |
| 412.90 | 5.14 | 5.14 |
| 426.92 | 5.39 | 5.39 |
| 385.87 | 4.31 | 4.31 |
| 399.90 | 4.70 | 4.70 |
| 386.86 | 4.34 | 4.34 |
| 400.89 | 5.00 | 5.00 |
| 412.90 | 5.14 | 5.14 |
| 446.90 | 6.54 | 6.54 |
| 412.90 | 5.14 | 5.14 |
| 480.03 | 6.37 | 6.37 |
| 439.97 | 5.48 | 5.48 |
| 425.94 | 5.23 | 5.23 |
| 426.92 | 5.26 | 5.26 |
| 565.05 | 6.55 | 6.55 |
| 538.46 | 6.83 | 6.83 |
| 484.02 | 5.51 | 5.51 |
| 509.07 | 5.52 | 5.52 |
| 371.85 | 3.92 | 3.92 |
| 372.83 | 3.95 | 3.95 |
| 427.96 | 5.30 | 5.30 |
| 425.94 | 4.96 | 4.96 |
| 439.97 | 5.30 | 5.30 |
| 425.94 | 4.96 | 4.96 |
| 439.97 | 5.35 | 5.35 |
| 439.97 | 5.35 | 5.35 |
| 440.95 | 3.87 | 3.87 |
| 439.97 | 5.48 | 5.48 |
| 454.98 | 4.21 | 4.21 |
| 441.94 | 4.29 | 4.29 |
| 370.86 | 5.37 | 5.37 |
| 384.89 | 5.76 | 5.76 |
| 440.95 | 5.78 | 5.78 |
| 433.92 | 5.60 | 5.60 |
| 426.92 | 5.39 | 5.39 |
| 433.92 | 5.60 | 5.60 |
| 342.81 | 4.59 | 4.59 |
| 328.78 | 4.13 | 4.13 |
| 421.52 | 4.59 | 4.59 |
| 440.95 | 5.51 | 5.51 |
| 426.92 | 5.26 | 5.26 |
| 412.90 | 4.87 | 4.87 |
| 412.90 | 4.87 | 4.87 |
| 408.91 | 6.07 | 6.07 |
| 400.89 | 4.73 | 4.73 |
| 442.92 | 5.30 | 5.30 |
| 417.89 | 4.84 | 4.84 |
| 404.85 | 4.48 | 4.48 |
| 454.98 | 6.17 | 6.17 |
| 411.91 | 4.84 | 4.84 |
| 411.91 | 4.84 | 4.84 |
| 502.01 | 5.65 | 5.65 |
| 440.95 | 5.92 | 5.92 |
| 401.87 | 3.28 | 3.28 |
| 386.81 | 4.04 | 4.04 |
| 409.48 | 4.72 | 4.72 |
| 471.98 | 5.97 | 5.97 |
| 498.05 | 5.85 | 5.85 |
| 484.02 | 5.24 | 5.24 |
| 440.91 | 5.21 | 5.21 |
| 440.91 | 5.21 | 5.21 |
| 416.96 | 3.71 | 3.71 |
| 453.95 | 4.87 | 4.87 |
| 467.98 | 5.22 | 5.22 |
| 549.12 | 5.86 | 5.86 |
| 549.12 | 5.86 | 5.86 |
| 417.94 | 3.75 | 3.75 |
| 430.98 | 3.78 | 3.78 |
| 363.85 | 2.43 | 2.43 |
| 405.52 | 4.89 | 4.89 |
| 427.98 | 4.46 | 4.46 |
| 494.01 | 5.75 | 5.75 |
| 453.95 | 4.87 | 4.87 |
| 457.02 | 4.15 | 4.15 |
| 422.58 | 3.50 | 3.50 |
| 457.02 | 4.15 | 4.15 |
| 422.58 | 3.50 | 3.50 |
| 426.92 | 5.12 | 5.12 |
| 426.92 | 5.12 | 5.12 |
| 451.40 | 4.37 | 4.37 |
| 452.39 | 4.40 | 4.40 |
| 451.40 | 4.37 | 4.37 |
| 459.98 | 2.81 | 2.81 |
| 505.49 | 5.50 | 5.50 |
| 431.97 | 3.29 | 3.29 |
| 432.96 | 3.33 | 3.33 |
| 416.96 | 3.71 | 3.71 |
| 417.94 | 3.75 | 3.75 |
| 464.04 | 3.72 | 3.72 |
| 430.98 | 4.02 | 4.02 |
| 416.96 | 3.71 | 3.71 |
| 430.98 | 4.02 | 4.02 |
| 425.94 | 5.09 | 5.09 |
| 439.97 | 5.35 | 5.35 |
| 453.99 | 5.74 | 5.74 |
| 425.94 | 5.09 | 5.09 |
| 439.97 | 5.35 | 5.35 |
| 453.99 | 5.74 | 5.74 |
| 457.02 | 4.59 | 4.59 |
| 445.01 | 4.27 | 4.27 |
| 431.97 | 3.99 | 3.99 |
| 475.04 | 3.99 | 3.99 |
| 458.99 | 3.89 | 3.89 |
| 495.05 | 3.30 | 3.30 |
| 430.98 | 3.96 | 3.96 |
| 473.02 | 4.14 | 4.14 |
| 430.98 | 3.74 | 3.74 |
| 445.01 | 3.98 | 3.98 |
| 431.97 | 3.77 | 3.77 |
| 446.00 | 4.02 | 4.02 |
| 489.06 | 4.01 | 4.01 |
| 450.41 | 4.97 | 4.97 |
| 556.56 | 4.65 | 4.65 |
| 484.95 | 4.73 | 4.73 |
| 444.02 | 5.10 | 5.10 |
| 444.02 | 5.10 | 5.10 |
| 508.49 | 5.25 | 5.25 |
| 494.47 | 4.59 | 4.59 |
| 433.96 | 4.46 | 4.46 |
| 416.96 | 3.71 | 3.71 |
| 416.96 | 3.71 | 3.71 |
| 417.94 | 3.75 | 3.75 |
| 417.94 | 3.75 | 3.75 |
| 415.97 | 4.32 | 4.32 |
| 484.86 | 5.62 | 5.62 |
| 451.40 | 5.00 | 5.00 |
| 534.53 | 5.78 | 5.78 |
| 433.96 | 4.46 | 4.46 |
| 534.53 | 5.78 | 5.78 |
| 550.53 | 5.02 | 5.02 |
| 522.52 | 5.59 | 5.59 |
| 500.09 | 5.13 | 5.13 |
| 474.05 | 4.59 | 4.59 |
| 416.95 | 4.35 | 4.35 |
| 474.05 | 4.59 | 4.59 |
| 500.09 | 5.13 | 5.13 |
| 500.09 | 5.13 | 5.13 |
| 488.08 | 4.94 | 4.94 |
| 520.51 | 5.39 | 5.39 |
| 490.02 | 4.49 | 4.49 |
| 433.96 | 4.46 | 4.46 |
| 492.04 | 4.73 | 4.73 |
| 444.02 | 5.09 | 5.09 |
| 502.10 | 5.37 | 5.37 |
| 494.87 | 5.08 | 5.08 |
| 488.08 | 4.98 | 4.98 |
| 528.02 | 4.87 | 4.87 |
| 528.02 | 4.87 | 4.87 |
| 562.46 | 5.52 | 5.52 |
| 522.52 | 5.64 | 5.64 |
| 552.95 | 5.36 | 5.36 |
| 506.07 | 5.12 | 5.12 |
| 506.07 | 5.12 | 5.12 |
| 451.40 | 5.00 | 5.00 |
| 479.45 | 5.78 | 5.78 |
| 590.52 | 6.30 | 6.30 |
| 590.52 | 6.30 | 6.30 |
| 415.97 | 4.05 | 4.05 |
| 528.02 | 5.48 | 5.48 |
| 562.46 | 6.14 | 6.14 |
| 401.94 | 3.80 | 3.80 |
| 401.94 | 3.80 | 3.80 |
| 518.10 | 4.75 | 4.75 |
| 516.13 | 5.91 | 5.91 |
| 518.10 | 4.75 | 4.75 |
| 556.07 | 6.00 | 6.00 |
| 556.07 | 6.00 | 6.00 |
| 513.09 | 4.88 | 4.88 |
| 430.00 | 4.71 | 4.71 |
| 447.99 | 4.85 | 4.85 |
| 433.96 | 4.41 | 4.41 |
| 492.04 | 4.73 | 4.73 |
| 567.67 | 6.27 | 6.27 |
| 518.10 | 4.75 | 4.75 |
| 451.95 | 4.60 | 4.60 |
| 554.13 | 4.52 | 4.52 |
| 564.56 | 5.75 | 5.75 |
| 522.52 | 5.78 | 5.78 |
| 552.12 | 4.27 | 4.27 |
| 522.52 | 5.64 | 5.64 |
| 504.05 | 4.88 | 4.88 |
| 522.52 | 5.78 | 5.78 |
| 540.11 | 4.13 | 4.13 |
| 522.52 | 5.64 | 5.64 |
| 516.13 | 5.76 | 5.76 |
| 516.13 | 5.76 | 5.76 |
| 516.13 | 5.76 | 5.76 |
| 580.17 | 4.91 | 4.91 |
| 566.14 | 4.66 | 4.66 |
| 520.05 | 4.81 | 4.81 |
| 534.08 | 5.20 | 5.20 |
| 545.06 | 4.70 | 4.70 |
| 517.05 | 4.63 | 4.63 |
| 531.08 | 5.02 | 5.02 |
| 458.97 | 4.35 | 4.35 |
| 501.05 | 5.39 | 5.39 |
| 543.13 | 6.51 | 6.51 |
| 580.17 | 5.05 | 5.05 |
| 552.12 | 4.27 | 4.27 |
| 582.60 | 5.18 | 5.18 |
| 518.10 | 4.35 | 4.35 |
| 504.08 | 3.96 | 3.96 |
| 459.95 | 4.38 | 4.38 |
| 441.96 | 4.24 | 4.24 |
| 469.03 | 4.86 | 4.86 |
| 469.03 | 4.81 | 4.81 |
| 553.03 | 5.38 | 5.38 |
| 525.10 | 5.02 | 5.02 |
| 492.04 | 4.69 | 4.69 |
| 550.17 | 4.77 | 4.77 |
| 497.09 | 5.59 | 5.59 |
| 496.03 | 3.30 | 3.30 |