Basic Information
ID: ALA3705382
Type: Binding
Description: Binding Assay: Cells expressing recombinant human CL receptor/RAMP1 were washed with PBS and harvested in harvest buffer containing 50 mM HEPES, 1 mM EDTA and Complete protease inhibitors (Roche). The cell suspension was disrupted with a laboratory homogenizer and centrifuged at 48,000 g to isolate membranes. The pellets were resuspended in harvest buffer plus 250 mM sucrose and stored at −70C. For binding assays, 20 μg of membranes were incubated in 1 ml binding buffer (10 mM HEPES, pH 7.4, 5 mM MgCl2, and 0.2% BSA) for 3 hours at room temperature containing 10 pM 125I-hCGRP (GE Healthcare) and antagonist. The assay was terminated by filtration through 96-well GFB glass fiber filter plates (PerkinElmer) that had been blocked with 0.05% polyethyleneimine. The filters were washed 3 times with ice-cold assay buffer (10 mM HEPES, pH 7.4 and 5 mM MgCl2). Scintillation fluid was added and the plates were counted on a Topeount (Packard).
Organism: Homo sapiens
Activity Charts
Bioactivity
Activity Types for Assay ALA3705382
Ki
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 517.63 | 3.94 | 3.94 |
| 479.56 | 3.90 | 3.90 |
| 434.50 | 4.02 | 4.02 |
| 543.67 | 4.47 | 4.47 |
| 461.57 | 3.77 | 3.77 |
| 557.70 | 4.86 | 4.86 |
| 565.68 | 4.90 | 4.90 |
| 543.67 | 4.47 | 4.47 |
| 385.47 | 2.02 | 2.02 |
| 529.64 | 4.60 | 4.60 |
| 561.69 | 5.41 | 5.41 |
| 425.54 | 2.61 | 2.61 |
| 455.52 | 2.99 | 2.99 |
| 454.57 | 3.71 | 3.71 |
| 516.60 | 4.62 | 4.62 |
| 488.59 | 4.64 | 4.64 |
| 453.59 | 4.07 | 4.07 |
| 420.56 | 2.38 | 2.38 |
| 443.55 | 2.72 | 2.72 |
| 471.56 | 4.66 | 4.66 |