Basic Information
ID: ALA3705428
Type: Binding
Description: Inhibtion Assay: Assays (200 μL final volume) were carried out in NUNC polypropylene deep well plates in 50 mM potassium phosphate buffer, pH 7.4, using a microtiter plate shaker in a 37° C. incubator. Pooled human liver microsomes (BD Gentest, 50 μg/mL) were incubated with 5 concentrations of test compound (from 0.1 μM to 10 μM), 1 mM NADPH (Sigma), and 2 μM midazolam (Sigma). A constant amount of dimethylsulfoxide (1%) was added to the incubations with the test compounds, and each analysis was performed in duplicate. For preincubation experiments (Pre), the microsomes, test compounds, and NADPH were mixed and incubated 30 minutes before addition of midazolam. For coincubation experiments (Co), the compounds, microsomes, and midazolam were mixed and the reaction initiated by addition of NADPH to the wells.
Organism: Homo sapiens
Tissue: Liver
Activity Charts
Bioactivity
Activity Types for Assay ALA3705428
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 440.53 | 5.89 | 5.89 |
| 488.55 | 5.18 | 5.18 |
| 528.64 | 5.83 | 5.83 |
| 498.61 | 5.74 | 5.74 |
| 530.61 | 4.41 | 4.41 |