Basic Information
ID: ALA3705429
Type: Binding
Description: Biochemical Assay: The inhibitory activity of putative kinase inhibitors and the potency of selected compounds were determined using a trans-phosphorylation assay.A specific substrate was incubated with the kinase in appropriate buffer conditions in the presence of ATP traced with .sup.33P-.gamma.-ATP (gamma phosphate-labeled, Redivue.TM. Code Number AH9968, 1000-3000 Ci/mmole, Amersham Biosciences Piscataway, N.J., USA), optimal cofactors and test compound.At the end of the phosphorylation reaction, more than 98% cold and radioactive ATP were captured by an excess of Dowex ion exchange resin. The resin was allowed to settle to the bottom of reaction wells by gravity. Supernatant, containing substrate peptide, was subsequently withdrawn and transferred into a counting plate, and radioactivity (corresponding to phosphate incorporated into peptide) was evaluated by .beta.-counting. Assay Conditions:The kinase assay was run with a final enzyme concentration of 6 nM, in the presence of 6 microM ATP, 1 nM .
Format: BAO_0000357
Organism: Homo sapiens
Target: Insulin-like growth factor I receptor(ALA1957)
Document: ALA3638444
BindingDB Source: 6899
Activity Charts
Bioactivity
Activity Types for Assay ALA3705429
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 560.65 | 5.03 | 5.03 |
| 461.52 | 4.44 | 4.44 |
| 534.61 | 4.49 | 4.49 |
| 558.68 | 6.18 | 6.18 |