Assay Report Card

Basic Information

ID: ALA3705439

Type: Binding

Description: Inhibition Assay: Fluorometric Assays: All measurements were performed with a BioAssay Reader HTS-7000Plus for microplates (Perkin Elmer) at 30 C. QC activity was evaluated fluorometrically using H-Gln-beta NA. The samples consisted of 0.2 mM fluorogenic substrate, 0.25 U pyroglutamyl aminopeptidase (Unizyme, Horsholm, Denmark) in 0.2 M Tris/HCl, pH 8.0 containing 20 mM EDTA and an appropriately diluted aliquot of QC in a final volume of 250 ul. Excitation/emission wavelengths were 320/410 nm. The assay reactions were initiated by addition of glutaminyl cyclase. QC activity was determined from a standard curve of beta -naphthylamine under assay conditions. One unit is defined as the amount of QC catalyzing the formation of 1 umol pGlu-beta NA from H-Gln-beta NA per minute under the described conditions.In a second fluorometric assay, QC was activity determined using H-Gln-AMC as substrate. Reactions were carried out at 30 C. utilizing the NOVOStar reader for microplates (BMG labtechnology).

Organism: Homo sapiens

Activity Charts

Bioactivity

Activity Types for Assay ALA3705439

Total(67)

Compound Summary

Parent Molecular Weight	ALogP	Polar Surface Area
286.36	2.35	2.35
300.39	2.65	2.65
320.44	3.02	3.02
360.51	3.59	3.59
334.47	3.28	3.28
334.47	3.33	3.33
348.50	3.59	3.59
382.51	4.38	4.38
346.48	3.20	3.20
374.54	3.84	3.84
374.54	3.98	3.98
388.56	4.37	4.37
396.54	4.69	4.69
396.54	4.31	4.31
300.39	2.65	2.65
320.44	3.02	3.02
360.51	3.59	3.59
334.47	3.28	3.28
334.47	3.33	3.33
348.50	3.59	3.59
382.51	4.38	4.38
346.48	3.20	3.20
374.54	3.84	3.84
374.54	3.98	3.98
388.56	4.37	4.37
396.54	4.69	4.69
396.54	4.31	4.31
306.42	2.72	2.72
306.42	2.72	2.72
346.48	3.29	3.29
334.47	3.28	3.28
332.45	2.90	2.90
374.54	4.07	4.07
368.49	3.92	3.92
294.34	2.92	2.92
314.40	3.29	3.29