Assay Report Card

Basic Information

ID: ALA3705440

Type: Binding

Description: Inhibition Assay: For sodium-dependent glucose transport assay, cells expressing hSGLT2 were seeded into a 96-well culture plate at a density of 5x104 cells/well in RPMI medium 1640 containing 10% fetal bovine serum. The cells were used 1 day after plating. They were incubated in pretreatment buffer (10 mM HEPES, 5 mM Tris, 140 mM choline chloride, 2 mM KCl, 1 mM CaCl2, and 1 mM MgCl2, pH 7.4) at 37 C. for 10 min. They were then incubated in uptake buffer (10 mM HEPES, 5 mM Tris, 140 mM NaCl, 2 mM KCl, 1 mM CaCl2, 1 mM MgCl2, and 1 mM 14C-nonlabeled AMG pH 7.4) containing 14C-labeled AMG (8 uM) and the inventive compound or dimethyl sulfoxide (DMSO) vehicle at 37 C. for 2 h. Cells were washed twice with washing buffer (pretreatment buffer containing 10 mM AMG at room temperature) and then the radioactivity was measured using a liquid scintillation counter. IC50 was determined by nonlinear regression analysis using GraphPad PRISM [Katsuno, K. et al. J. Pharmacol. Exp. Ther. 2007, 320, 323-330].

Organism: Homo sapiens

Activity Charts

Compound Summary

Parent Molecular WeightALogPPolar Surface Area
449.931.451.45
464.901.151.15
422.861.091.09
480.941.141.14
464.942.172.17
450.871.031.03
452.890.880.88
449.931.451.45
506.981.941.94
461.941.591.59
490.862.282.28
478.932.522.52
464.902.212.21
445.901.451.45
478.931.161.16
464.942.172.17
478.992.642.64
442.921.451.45
435.952.292.29
449.931.281.28
465.931.651.65
479.961.671.67
449.931.811.81
448.942.502.50
450.921.951.95
462.972.682.68
464.942.122.12
464.942.172.17
478.972.562.56
433.932.052.05
478.972.512.51
464.942.342.34
478.972.512.51
464.901.421.42
480.941.921.92
456.542.002.00
458.552.082.08
461.941.741.74
463.911.181.18
490.982.702.70
474.983.043.04
476.952.312.31
448.512.782.78
459.972.142.14
414.501.681.68
418.461.431.43
464.942.172.17
466.911.621.62
436.891.391.39
434.921.941.94
450.921.781.78
415.871.991.99
416.861.391.39
436.891.611.61
416.471.261.26
420.912.662.66
422.932.092.09
494.982.842.84
417.891.941.94
418.881.331.33
418.881.331.33
432.901.821.82
432.901.821.82
446.932.382.38
446.932.382.38
452.891.231.23
478.931.541.54
464.901.151.15
464.973.063.06
438.931.931.93
468.961.941.94
422.861.221.22
438.860.920.92
402.440.870.87
408.831.171.17
404.891.931.93
418.922.322.32
436.962.482.48
450.922.002.00
420.891.771.77
424.901.891.89
422.861.311.31
420.851.691.69
438.931.931.93
470.930.610.61
448.563.333.33
464.563.173.17
482.553.313.31
478.542.542.54
481.341.631.63
499.003.823.82
489.573.043.04
470.593.233.23
470.593.233.23
438.932.552.55
422.861.831.83
422.861.831.83
422.861.831.83
468.962.552.55
452.891.841.84
479.372.662.66
454.932.252.25
386.441.381.38
404.481.541.54
480.900.990.99
462.931.711.71
442.511.361.36
478.491.991.99
438.902.462.46
466.983.353.35
454.973.183.18
486.971.861.86
468.952.552.55
454.973.183.18
450.922.632.63
447.962.002.00
430.932.582.58
444.962.812.81
432.902.022.02
474.862.402.40
441.312.032.03
474.862.402.40
434.922.812.81
452.912.762.76
464.942.172.17
430.501.431.43
450.921.781.78
450.921.781.78
448.942.332.33
460.952.652.65
462.972.722.72
464.942.172.17
448.942.332.33
446.932.412.41
460.952.652.65
418.922.242.24
433.931.981.98
435.901.421.42
466.982.492.49
481.012.882.88
513.012.182.18
497.012.512.51
462.972.892.89
462.972.892.89
448.942.502.50
466.982.492.49
464.942.172.17
450.922.302.30
452.891.571.57
480.942.012.01
464.942.172.17
406.861.381.38
448.901.581.58
450.921.431.43
452.912.412.41
446.932.262.26
444.913.073.07
446.933.143.14
464.941.611.61
456.872.322.32
470.902.492.49
426.511.911.91
474.941.221.22
446.932.412.41
448.942.502.50
430.881.361.36
449.931.101.10
463.961.441.44
461.941.201.20
431.871.251.25
448.942.332.33
446.932.262.26
460.952.652.65
450.482.232.23
438.861.181.18
414.501.601.60
434.921.941.94
450.921.781.78
452.962.102.10
460.952.652.65
481.013.503.50
432.942.502.50
479.372.052.05
448.942.332.33
465.013.043.04
448.902.052.05
464.942.172.17
466.982.492.49
462.932.092.09
450.921.781.78
436.891.391.39
452.891.231.23
450.921.781.78
448.942.332.33
464.942.342.34
450.921.781.78
493.392.442.44
448.942.332.33
436.891.741.74
477.392.612.61
432.902.022.02
434.921.941.94