Basic Information
ID: ALA3705470
Type: Binding
Description: Inhibition Assay: The PDE9-inhibiting activity was measured by the following method. That is, to a buffer solution containing tris(hydroxymethyl)aminomethane-hydrochloric acid (40 mM, pH 8.0), magnesium chloride (5 mM), and 2-mercaptoethanol (4 mM) were added cGMP (1 μM) and 3H-cGMP (0.33 μCi/ml) to give a substrate buffer solution. A test substance solution and an enzyme solution which had been adjusted to an optimal concentration were added thereto to perform a reaction at 30° C. The enzyme reaction was stopped by the addition of Scintillation Proximity Assay (SPA) Beads (Perkin Elmer, USA) containing 5 mM 3-isobutyl-1-methylxanthine (IBMX). For the enzyme activity, the amount of 5′-GMP, which is a reaction degradation product bound to SPA beads, was measured with a TopCount microplate reader (Hewlett Packard, USA).
Activity Charts
Bioactivity
Activity Types for Assay ALA3705470
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 339.44 | 4.26 | 4.26 |
| 335.48 | 4.43 | 4.43 |
| 335.48 | 4.43 | 4.43 |
| 432.91 | 4.85 | 4.85 |
| 486.58 | 2.89 | 2.89 |
| 394.48 | 3.16 | 3.16 |
| 448.91 | 4.09 | 4.09 |
| 471.56 | 3.36 | 3.36 |
| 476.51 | 3.58 | 3.58 |
| 553.71 | 5.06 | 5.06 |
| 472.55 | 2.90 | 2.90 |
| 487.56 | 3.62 | 3.62 |
| 507.64 | 3.30 | 3.30 |
| 438.53 | 3.04 | 3.04 |
| 438.53 | 3.18 | 3.18 |
| 464.57 | 3.57 | 3.57 |
| 486.58 | 3.06 | 3.06 |
| 473.54 | 2.29 | 2.29 |
| 452.56 | 3.57 | 3.57 |
| 463.58 | 3.10 | 3.10 |
| 452.56 | 3.43 | 3.43 |
| 481.60 | 2.72 | 2.72 |
| 452.56 | 3.43 | 3.43 |
| 451.57 | 3.10 | 3.10 |
| 511.63 | 3.74 | 3.74 |
| 451.57 | 3.10 | 3.10 |
| 481.60 | 2.72 | 2.72 |
| 424.50 | 2.65 | 2.65 |
| 450.54 | 3.32 | 3.32 |
| 438.53 | 3.04 | 3.04 |
| 474.51 | 3.42 | 3.42 |
| 470.55 | 3.52 | 3.52 |
| 451.57 | 2.78 | 2.78 |
| 495.62 | 3.11 | 3.11 |
| 509.65 | 3.50 | 3.50 |
| 467.57 | 2.84 | 2.84 |
| 481.60 | 3.19 | 3.19 |
| 495.62 | 3.11 | 3.11 |
| 491.51 | 3.25 | 3.25 |
| 509.65 | 3.36 | 3.36 |
| 503.55 | 2.58 | 2.58 |
| 505.54 | 3.64 | 3.64 |
| 493.61 | 2.86 | 2.86 |
| 465.60 | 3.48 | 3.48 |
| 438.53 | 3.04 | 3.04 |
| 491.51 | 3.25 | 3.25 |
| 506.53 | 4.14 | 4.14 |
| 495.62 | 2.80 | 2.80 |
| 461.49 | 3.62 | 3.62 |
| 475.52 | 3.88 | 3.88 |
| 489.52 | 2.19 | 2.19 |
| 466.58 | 3.82 | 3.82 |
| 464.57 | 3.71 | 3.71 |
| 521.54 | 3.22 | 3.22 |
| 488.54 | 3.81 | 3.81 |
| 470.55 | 3.52 | 3.52 |
| 456.52 | 3.13 | 3.13 |
| 451.57 | 3.09 | 3.09 |
| 517.61 | 3.26 | 3.26 |
| 517.61 | 3.26 | 3.26 |
| 509.65 | 3.50 | 3.50 |
| 519.57 | 4.02 | 4.02 |
| 477.49 | 2.86 | 2.86 |
| 479.63 | 3.87 | 3.87 |
| 479.63 | 3.56 | 3.56 |
| 489.54 | 4.08 | 4.08 |
| 493.65 | 3.95 | 3.95 |
| 503.57 | 4.47 | 4.47 |
| 474.51 | 3.42 | 3.42 |
| 462.47 | 3.95 | 3.95 |
| 529.63 | 4.51 | 4.51 |
| 465.60 | 3.17 | 3.17 |
| 450.54 | 3.18 | 3.18 |
| 438.53 | 3.18 | 3.18 |
| 491.51 | 3.25 | 3.25 |
| 452.56 | 3.43 | 3.43 |
| 450.54 | 3.32 | 3.32 |
| 478.47 | 3.33 | 3.33 |
| 515.61 | 3.80 | 3.80 |
| 505.54 | 3.64 | 3.64 |
| 539.55 | 5.04 | 5.04 |
| 475.52 | 3.69 | 3.69 |
| 489.54 | 4.09 | 4.09 |
| 489.54 | 4.40 | 4.40 |
| 514.63 | 3.84 | 3.84 |
| 512.66 | 3.97 | 3.97 |
| 464.57 | 3.57 | 3.57 |
| 436.51 | 2.93 | 2.93 |
| 507.51 | 2.83 | 2.83 |
| 456.52 | 3.13 | 3.13 |
| 478.47 | 3.07 | 3.07 |
| 498.58 | 4.10 | 4.10 |
| 477.49 | 2.86 | 2.86 |
| 515.61 | 4.12 | 4.12 |
| 451.57 | 2.78 | 2.78 |
| 465.60 | 3.17 | 3.17 |
| 465.60 | 3.48 | 3.48 |
| 481.60 | 2.72 | 2.72 |
| 438.53 | 3.04 | 3.04 |
| 388.45 | 4.67 | 4.67 |