Basic Information
ID: ALA3705471
Type: Binding
Description: Homogeneous Time-Resolved Fluorescence Assay: Chinese hamster ovary (CHO) cells expressing either the human or rat recombinant CRF-1 or human CRF-2alpha (Chen et al, Proc Natl Acad Sci USA 90, 8967-8971, 1993; Liaw et al, Endocrinology 137, 72-77, 1996) are propagated in Dulbecco's modified Eagle medium supplemented with 10% foetal calf serum, non-essential amino acids, 100 U/ml penicillin, 100 mg/l streptomycin and 1 g/l geneticin. CHO cells expressing the rat CRF-2beta receptor (Wu et al, Endocrinology 148, 1675-1687, 2007) are propagated in HAM's-F12 Glutamax supplemented with 10% foetal calf serum, 100 IU/ml penicillin, 100 mg/l streptomycin, 600 ug/ml hygromycin, 10 ug/ml blasticidin and induced with 1 ug/ml of tetracyclin for 24 hours prior to experimentation. For cyclic AMP determinations the Homogeneous Time-Resolved Fluoresce (HTRF) CAMP dynamic 2 kit (Cisbio International, France) was used as per manufacturers' instructions.
Organism: Homo sapiens
Cell type: CHO
Activity Charts
Bioactivity
Activity Types for Assay ALA3705471
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 395.33 | 4.50 | 4.50 |
| 360.89 | 3.85 | 3.85 |
| 389.93 | 4.04 | 4.04 |
| 468.90 | 5.39 | 5.39 |
| 555.43 | 7.12 | 7.12 |
| 388.94 | 4.35 | 4.35 |
| 386.93 | 4.11 | 4.11 |
| 422.96 | 5.20 | 5.20 |
| 413.87 | 5.16 | 5.16 |
| 413.87 | 5.16 | 5.16 |
| 425.88 | 5.03 | 5.03 |
| 425.88 | 5.03 | 5.03 |
| 440.95 | 5.34 | 5.34 |
| 423.95 | 4.60 | 4.60 |
| 394.44 | 4.21 | 4.21 |
| 505.97 | 6.53 | 6.53 |
| 519.95 | 6.25 | 6.25 |
| 376.89 | 3.03 | 3.03 |
| 376.89 | 3.03 | 3.03 |
| 388.94 | 4.66 | 4.66 |
| 441.93 | 5.67 | 5.67 |
| 429.87 | 4.86 | 4.86 |
| 429.87 | 4.86 | 4.86 |
| 399.84 | 4.85 | 4.85 |
| 450.29 | 4.69 | 4.69 |
| 427.90 | 5.41 | 5.41 |
| 427.90 | 5.41 | 5.41 |
| 462.34 | 6.07 | 6.07 |