Basic Information
ID: ALA3705478
Type: Binding
Description: Fluorescence Based Assay: Compound inhibitory activity of FabI enzyme is measured in vitro by the IC5 so determination using a fluorescence based assay.The protein FabI from S. aureus is prepared and purified using standard methods for recombinant protein expression after cloning of the gene in a prokaryotic expression vector.The biochemical activity of the FabI enzyme is assessed using the following method.The assay buffer AB contained 50 mM ADA (N-(2-acetamido)iminodiacetic acid monosodium salt) pH 6.5, 1 mM dithiothreitol, 0.006% Triton-X100 and 50 mM NaCl.The following components are added in a white polystyrene Costar plate (Ref 3912) up to a final volume of 55.5 uL: 1.5 uL DMSO or inhibitor dissolved in DMSO and 54 uL of a FabI/NADPH/NADP+ mixture in AB. After 60 min of pre-incubation at room temperature, the reaction is started by addition of 5 uL of trans-2-octenoyl N-acetylcysteamine thioester (t-o-NAC) to a final volume of 60.5 uL.
Organism: Staphylococcus aureus
Activity Charts
Bioactivity
Activity Types for Assay ALA3705478
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 363.42 | 2.41 | 2.41 |
| 257.29 | 1.21 | 1.21 |
| 271.32 | 1.60 | 1.60 |
| 285.35 | 1.99 | 1.99 |
| 329.40 | 1.60 | 1.60 |
| 309.38 | 3.85 | 3.85 |
| 317.41 | 2.44 | 2.44 |
| 347.42 | 3.34 | 3.34 |
| 327.43 | 3.02 | 3.02 |
| 309.38 | 3.85 | 3.85 |
| 363.42 | 2.66 | 2.66 |
| 338.37 | 2.43 | 2.43 |
| 369.45 | 2.47 | 2.47 |
| 367.41 | 2.42 | 2.42 |
| 377.44 | 2.49 | 2.49 |
| 343.43 | 2.40 | 2.40 |
| 364.41 | 2.05 | 2.05 |
| 327.38 | 1.78 | 1.78 |
| 370.43 | 1.86 | 1.86 |
| 406.45 | 1.62 | 1.62 |
| 391.49 | 1.80 | 1.80 |
| 310.36 | 1.50 | 1.50 |
| 352.39 | 1.88 | 1.88 |
| 467.50 | 2.84 | 2.84 |
| 439.49 | 2.65 | 2.65 |
| 465.48 | 3.18 | 3.18 |
| 452.49 | 2.15 | 2.15 |
| 402.45 | 4.52 | 4.52 |