Basic Information
ID: ALA3705480
Type: Binding
Description: Radioligand Binding Assay: Radioligand binding assays were carried out in a volume of 200 mL (96-well plates) which contained 100 mL of cell memebranes, [3H]flumazenil at a concentration of 1 nM for α1, α2, α3 subunits and 0.5 nM for α5 subunits and the test compound in the range of 10-10−3x10−6 M. Nonspecific binding was defined by 10−5 M diazepam and typically represented less than 5% of the total binding. Assays were incubated to equilibrium for 1 hour at 4 C. and harvested onto GF/C uni-filters (Packard) by filtration using a Packard harvester and washing with ice-cold wash buffer (50 mM Tris; pH 7.5). After drying, filter-retained radioactivity was detected by liquid scintillation counting.
Organism: Homo sapiens
Activity Charts
Bioactivity
Activity Types for Assay ALA3705480
Ki
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 410.33 | 3.45 | 3.45 |
| 372.36 | 1.88 | 1.88 |
| 356.36 | 2.91 | 2.91 |
| 428.88 | 3.58 | 3.58 |
| 386.84 | 3.81 | 3.81 |
| 384.82 | 3.56 | 3.56 |
| 410.45 | 3.71 | 3.71 |
| 411.43 | 3.41 | 3.41 |
| 407.47 | 3.66 | 3.66 |
| 330.39 | 2.13 | 2.13 |
| 344.42 | 2.52 | 2.52 |
| 388.47 | 2.27 | 2.27 |
| 360.41 | 1.49 | 1.49 |
| 374.44 | 2.15 | 2.15 |
| 418.47 | 2.63 | 2.63 |
| 376.43 | 2.86 | 2.86 |
| 374.42 | 2.61 | 2.61 |
| 406.46 | 2.22 | 2.22 |
| 388.44 | 2.86 | 2.86 |
| 404.44 | 2.32 | 2.32 |
| 362.41 | 2.55 | 2.55 |
| 360.39 | 2.30 | 2.30 |
| 348.38 | 2.16 | 2.16 |
| 434.52 | 1.29 | 1.29 |
| 361.45 | 4.34 | 4.34 |
| 400.48 | 2.02 | 2.02 |
| 400.48 | 2.02 | 2.02 |
| 452.51 | 1.24 | 1.24 |
| 468.96 | 1.51 | 1.51 |
| 444.49 | 2.67 | 2.67 |
| 408.43 | 3.41 | 3.41 |
| 460.94 | 3.18 | 3.18 |
| 433.53 | 1.68 | 1.68 |
| 266.30 | 3.62 | 3.62 |
| 280.33 | 3.93 | 3.93 |
| 345.20 | 4.39 | 4.39 |
| 334.30 | 4.64 | 4.64 |
| 291.31 | 3.50 | 3.50 |
| 311.30 | 3.53 | 3.53 |
| 324.34 | 3.41 | 3.41 |
| 323.35 | 2.98 | 2.98 |
| 337.38 | 3.37 | 3.37 |
| 355.37 | 3.32 | 3.32 |
| 373.36 | 3.62 | 3.62 |
| 391.35 | 3.92 | 3.92 |
| 353.38 | 2.35 | 2.35 |
| 367.40 | 2.73 | 2.73 |
| 381.43 | 3.39 | 3.39 |
| 363.42 | 3.76 | 3.76 |
| 393.49 | 4.79 | 4.79 |
| 408.41 | 2.17 | 2.17 |
| 431.45 | 3.81 | 3.81 |
| 429.48 | 4.15 | 4.15 |
| 431.48 | 3.88 | 3.88 |
| 400.44 | 3.95 | 3.95 |
| 431.45 | 2.95 | 2.95 |
| 423.47 | 3.70 | 3.70 |
| 409.44 | 3.31 | 3.31 |
| 351.41 | 3.76 | 3.76 |
| 349.39 | 3.52 | 3.52 |
| 363.42 | 3.91 | 3.91 |
| 377.44 | 4.30 | 4.30 |
| 391.47 | 4.69 | 4.69 |
| 393.44 | 3.53 | 3.53 |
| 421.50 | 4.31 | 4.31 |
| 441.51 | 2.93 | 2.93 |
| 406.49 | 3.45 | 3.45 |
| 420.51 | 3.84 | 3.84 |
| 434.54 | 4.23 | 4.23 |
| 482.58 | 5.02 | 5.02 |
| 492.58 | 4.75 | 4.75 |
| 420.51 | 3.84 | 3.84 |
| 478.55 | 3.38 | 3.38 |
| 450.50 | 2.90 | 2.90 |
| 477.57 | 2.95 | 2.95 |
| 489.58 | 3.10 | 3.10 |
| 531.54 | 3.50 | 3.50 |
| 493.56 | 1.93 | 1.93 |
| 533.63 | 3.11 | 3.11 |
| 365.43 | 4.15 | 4.15 |
| 385.42 | 4.88 | 4.88 |
| 403.41 | 5.02 | 5.02 |
| 407.47 | 3.88 | 3.88 |
| 377.44 | 4.25 | 4.25 |
| 413.42 | 4.50 | 4.50 |
| 379.42 | 3.10 | 3.10 |
| 395.48 | 3.81 | 3.81 |
| 372.42 | 5.20 | 5.20 |
| 391.35 | 3.92 | 3.92 |
| 393.44 | 3.53 | 3.53 |
| 407.47 | 3.84 | 3.84 |
| 378.31 | 4.34 | 4.34 |
| 419.40 | 4.78 | 4.78 |
| 461.44 | 4.55 | 4.55 |
| 403.23 | 4.17 | 4.17 |
| 472.34 | 4.30 | 4.30 |
| 407.47 | 3.84 | 3.84 |
| 486.37 | 4.60 | 4.60 |
| 430.30 | 4.52 | 4.52 |
| 365.43 | 4.07 | 4.07 |
| 365.43 | 4.10 | 4.10 |
| 407.47 | 3.88 | 3.88 |
| 352.39 | 4.11 | 4.11 |
| 291.31 | 3.50 | 3.50 |
| 338.36 | 2.84 | 2.84 |
| 416.44 | 2.87 | 2.87 |
| 407.47 | 3.78 | 3.78 |
| 393.44 | 3.39 | 3.39 |
| 415.45 | 3.87 | 3.87 |
| 441.49 | 4.63 | 4.63 |
| 432.48 | 4.67 | 4.67 |
| 391.43 | 3.10 | 3.10 |
| 391.43 | 3.24 | 3.24 |
| 352.39 | 4.19 | 4.19 |
| 409.34 | 4.06 | 4.06 |
| 459.35 | 4.69 | 4.69 |
| 369.40 | 3.90 | 3.90 |
| 411.43 | 3.67 | 3.67 |
| 342.33 | 3.55 | 3.55 |
| 409.34 | 4.06 | 4.06 |
| 381.41 | 3.90 | 3.90 |
| 369.40 | 3.90 | 3.90 |
| 367.38 | 3.66 | 3.66 |
| 411.43 | 3.67 | 3.67 |
| 397.41 | 3.24 | 3.24 |
| 413.47 | 3.95 | 3.95 |
| 445.47 | 2.63 | 2.63 |
| 358.78 | 4.06 | 4.06 |
| 425.79 | 4.57 | 4.57 |
| 397.86 | 4.42 | 4.42 |
| 383.84 | 4.17 | 4.17 |
| 427.89 | 4.19 | 4.19 |
| 461.93 | 3.15 | 3.15 |
| 342.33 | 3.55 | 3.55 |
| 328.30 | 3.46 | 3.46 |
| 409.34 | 4.06 | 4.06 |
| 381.41 | 3.90 | 3.90 |
| 369.40 | 3.90 | 3.90 |
| 367.38 | 3.66 | 3.66 |
| 411.43 | 3.67 | 3.67 |
| 397.41 | 3.24 | 3.24 |
| 413.47 | 3.95 | 3.95 |
| 445.47 | 2.63 | 2.63 |
| 356.35 | 2.98 | 2.98 |
| 358.78 | 4.06 | 4.06 |
| 344.75 | 3.98 | 3.98 |
| 425.79 | 4.57 | 4.57 |
| 397.86 | 4.42 | 4.42 |
| 385.85 | 4.42 | 4.42 |
| 383.84 | 4.17 | 4.17 |
| 427.89 | 4.19 | 4.19 |
| 413.86 | 3.75 | 3.75 |
| 429.93 | 4.47 | 4.47 |
| 427.33 | 4.19 | 4.19 |
| 387.39 | 4.04 | 4.04 |
| 429.42 | 3.81 | 3.81 |
| 378.31 | 4.12 | 4.12 |
| 377.32 | 3.69 | 3.69 |
| 391.35 | 4.08 | 4.08 |
| 407.35 | 3.06 | 3.06 |
| 417.39 | 4.47 | 4.47 |
| 405.38 | 4.47 | 4.47 |
| 403.36 | 4.23 | 4.23 |
| 447.41 | 4.24 | 4.24 |
| 396.30 | 4.26 | 4.26 |
| 395.31 | 3.83 | 3.83 |
| 409.34 | 4.22 | 4.22 |
| 425.34 | 3.20 | 3.20 |
| 463.31 | 4.77 | 4.77 |
| 435.38 | 4.61 | 4.61 |
| 423.37 | 4.61 | 4.61 |
| 421.35 | 4.37 | 4.37 |
| 465.40 | 4.38 | 4.38 |
| 499.44 | 3.34 | 3.34 |
| 392.34 | 3.31 | 3.31 |
| 364.41 | 3.16 | 3.16 |
| 350.38 | 2.91 | 2.91 |
| 352.39 | 3.16 | 3.16 |
| 394.43 | 2.93 | 2.93 |
| 380.40 | 2.49 | 2.49 |
| 396.47 | 3.21 | 3.21 |
| 428.47 | 1.89 | 1.89 |
| 325.32 | 2.81 | 2.81 |
| 392.34 | 3.31 | 3.31 |
| 364.41 | 3.16 | 3.16 |
| 350.38 | 2.91 | 2.91 |
| 352.39 | 3.16 | 3.16 |
| 394.43 | 2.93 | 2.93 |
| 265.32 | 3.66 | 3.66 |
| 323.35 | 3.44 | 3.44 |
| 352.39 | 2.38 | 2.38 |
| 419.49 | 3.01 | 3.01 |
| 350.42 | 3.80 | 3.80 |
| 348.41 | 3.55 | 3.55 |
| 392.46 | 3.57 | 3.57 |
| 341.34 | 3.58 | 3.58 |
| 408.36 | 4.09 | 4.09 |
| 368.41 | 3.93 | 3.93 |
| 366.40 | 3.69 | 3.69 |
| 341.34 | 3.58 | 3.58 |
| 408.36 | 4.09 | 4.09 |
| 380.42 | 3.94 | 3.94 |
| 368.41 | 3.93 | 3.93 |
| 366.40 | 3.69 | 3.69 |
| 410.45 | 3.71 | 3.71 |
| 323.35 | 3.58 | 3.58 |
| 367.36 | 2.74 | 2.74 |
| 351.41 | 4.22 | 4.22 |
| 349.39 | 3.97 | 3.97 |
| 381.43 | 3.12 | 3.12 |
| 367.40 | 3.00 | 3.00 |
| 427.48 | 2.54 | 2.54 |
| 421.38 | 3.28 | 3.28 |
| 393.44 | 3.22 | 3.22 |
| 395.46 | 3.37 | 3.37 |
| 395.46 | 3.78 | 3.78 |
| 367.40 | 2.73 | 2.73 |
| 365.39 | 2.44 | 2.44 |
| 407.47 | 3.66 | 3.66 |
| 381.43 | 3.12 | 3.12 |
| 379.42 | 2.88 | 2.88 |
| 367.40 | 2.73 | 2.73 |
| 367.40 | 2.73 | 2.73 |
| 407.47 | 3.66 | 3.66 |
| 393.44 | 3.27 | 3.27 |
| 383.40 | 1.71 | 1.71 |
| 379.42 | 3.14 | 3.14 |
| 407.47 | 3.66 | 3.66 |
| 407.47 | 3.66 | 3.66 |
| 394.43 | 2.49 | 2.49 |
| 395.46 | 3.37 | 3.37 |
| 409.49 | 3.76 | 3.76 |
| 381.43 | 3.12 | 3.12 |
| 381.43 | 3.12 | 3.12 |
| 393.44 | 3.27 | 3.27 |
| 393.44 | 3.27 | 3.27 |
| 393.44 | 3.27 | 3.27 |
| 393.44 | 3.27 | 3.27 |
| 421.46 | 2.38 | 2.38 |
| 381.43 | 3.12 | 3.12 |
| 464.52 | 3.97 | 3.97 |
| 452.51 | 3.88 | 3.88 |
| 383.40 | 1.71 | 1.71 |
| 367.41 | 2.74 | 2.74 |
| 381.43 | 3.13 | 3.13 |
| 395.46 | 3.52 | 3.52 |
| 409.49 | 3.91 | 3.91 |
| 379.42 | 2.83 | 2.83 |
| 469.54 | 5.10 | 5.10 |
| 377.44 | 4.25 | 4.25 |
| 363.42 | 3.86 | 3.86 |
| 471.51 | 4.14 | 4.14 |
| 457.49 | 4.05 | 4.05 |
| 379.42 | 3.14 | 3.14 |
| 429.50 | 3.48 | 3.48 |
| 405.38 | 4.30 | 4.30 |
| 405.38 | 4.30 | 4.30 |
| 427.48 | 3.19 | 3.19 |
| 389.42 | 3.61 | 3.61 |
| 376.37 | 2.80 | 2.80 |
| 363.42 | 3.91 | 3.91 |
| 349.39 | 3.47 | 3.47 |
| 379.42 | 3.09 | 3.09 |
| 381.46 | 3.77 | 3.77 |
| 374.40 | 3.41 | 3.41 |
| 389.42 | 3.61 | 3.61 |
| 348.36 | 3.98 | 3.98 |
| 347.38 | 3.72 | 3.72 |
| 312.39 | 4.35 | 4.35 |
| 328.39 | 3.36 | 3.36 |
| 407.47 | 3.84 | 3.84 |
| 365.43 | 4.07 | 4.07 |
| 407.47 | 3.84 | 3.84 |
| 365.43 | 4.07 | 4.07 |
| 383.42 | 4.21 | 4.21 |
| 415.88 | 3.78 | 3.78 |
| 423.86 | 4.26 | 4.26 |
| 401.85 | 3.39 | 3.39 |
| 401.85 | 3.39 | 3.39 |
| 439.82 | 4.96 | 4.96 |
| 427.89 | 3.92 | 3.92 |
| 427.89 | 3.92 | 3.92 |
| 387.39 | 4.04 | 4.04 |
| 429.42 | 3.81 | 3.81 |
| 387.39 | 4.04 | 4.04 |
| 429.42 | 3.81 | 3.81 |
| 387.39 | 4.04 | 4.04 |
| 429.42 | 3.81 | 3.81 |
| 417.41 | 3.40 | 3.40 |
| 431.44 | 3.65 | 3.65 |
| 417.41 | 3.40 | 3.40 |
| 403.84 | 4.55 | 4.55 |
| 325.32 | 2.81 | 2.81 |
| 394.43 | 2.93 | 2.93 |
| 352.39 | 3.16 | 3.16 |
| 380.40 | 2.49 | 2.49 |
| 392.34 | 3.31 | 3.31 |
| 364.41 | 3.16 | 3.16 |
| 350.38 | 2.91 | 2.91 |
| 396.47 | 3.21 | 3.21 |
| 311.30 | 2.72 | 2.72 |
| 343.31 | 2.94 | 2.94 |
| 329.29 | 2.86 | 2.86 |
| 398.39 | 2.63 | 2.63 |
| 359.77 | 3.46 | 3.46 |
| 345.74 | 3.37 | 3.37 |
| 416.87 | 3.01 | 3.01 |
| 424.35 | 3.84 | 3.84 |
| 342.33 | 2.39 | 2.39 |
| 398.85 | 3.86 | 3.86 |
| 426.78 | 4.01 | 4.01 |
| 414.85 | 3.14 | 3.14 |
| 430.92 | 3.86 | 3.86 |
| 388.81 | 2.27 | 2.27 |
| 326.31 | 2.20 | 2.20 |
| 353.38 | 2.55 | 2.55 |
| 351.37 | 2.31 | 2.31 |
| 383.41 | 1.91 | 1.91 |
| 381.39 | 1.89 | 1.89 |
| 339.36 | 2.16 | 2.16 |
| 325.33 | 1.77 | 1.77 |
| 397.46 | 2.60 | 2.60 |
| 355.35 | 1.14 | 1.14 |
| 337.38 | 3.45 | 3.45 |
| 379.42 | 3.22 | 3.22 |
| 355.37 | 3.59 | 3.59 |
| 397.41 | 3.36 | 3.36 |
| 371.37 | 2.57 | 2.57 |
| 371.37 | 2.57 | 2.57 |
| 371.37 | 2.57 | 2.57 |
| 367.38 | 3.59 | 3.59 |
| 353.35 | 3.35 | 3.35 |
| 395.31 | 3.75 | 3.75 |
| 397.41 | 3.10 | 3.10 |
| 397.41 | 3.10 | 3.10 |
| 425.34 | 3.11 | 3.11 |
| 387.37 | 1.54 | 1.54 |
| 398.39 | 2.32 | 2.32 |
| 371.37 | 2.83 | 2.83 |
| 371.37 | 2.57 | 2.57 |
| 357.34 | 2.18 | 2.18 |
| 383.38 | 2.71 | 2.71 |
| 431.45 | 2.37 | 2.37 |
| 411.77 | 4.26 | 4.26 |
| 369.81 | 3.86 | 3.86 |
| 371.82 | 4.11 | 4.11 |
| 413.86 | 3.88 | 3.88 |
| 373.80 | 2.69 | 2.69 |
| 387.82 | 3.08 | 3.08 |
| 387.82 | 3.08 | 3.08 |
| 401.85 | 3.47 | 3.47 |
| 415.88 | 3.72 | 3.72 |
| 484.94 | 4.32 | 4.32 |
| 413.86 | 3.61 | 3.61 |
| 403.82 | 2.05 | 2.05 |
| 387.82 | 3.08 | 3.08 |
| 387.82 | 3.08 | 3.08 |
| 414.85 | 2.83 | 2.83 |
| 425.79 | 4.65 | 4.65 |
| 380.40 | 2.62 | 2.62 |
| 338.37 | 2.85 | 2.85 |
| 336.35 | 2.60 | 2.60 |
| 350.38 | 2.85 | 2.85 |
| 378.31 | 3.00 | 3.00 |
| 340.34 | 1.43 | 1.43 |
| 324.34 | 2.46 | 2.46 |
| 386.38 | 2.18 | 2.18 |
| 396.30 | 3.19 | 3.19 |
| 358.33 | 1.45 | 1.45 |
| 328.30 | 2.09 | 2.09 |