Basic Information
ID: ALA3705519
Type: Binding
Description: Reverse Mode Assay: The assay is based on the monitoring of intracellular Ca2+concentrations using the calcium-sensitive dye Fluo-4. CHO cells expressing NCX1 were loaded with the dye by means of the acetoxymethyl ester Fluo-4 AM (Invitrogen, F14202), which is cleaved intracellularly by esterase activity to yield the charged species of free Fluo-4. After an preincubation period with the test compound, Gramicidine (Sigma, G5002) was added. Gramicidine is an ionophor for Na+ions mediating an increase of intracellular Na+ions. Consequently, intracellular Na+ions are exchanged against extracellular Ca2+ions (Ca2+influx, reverse mode). The intracellular elevation of Ca2+ ions was detected by measuring the fluorescence of Fluo-4 at a wavelength of 520 nm by a FLIPR device.Briefly, for the reverse mode transport assay 18000 cells per well were seeded into a 96 well microplate (Corning COSTAR 3904) and incubated overnight in culture medium (1x Nut Mix F12 (Ham) (Gibco, 21765-029); 10% (v/v) fetal calf serum.
Format: BAO_0000219
Cell type: CHO
Target: Sodium/calcium exchanger 1(ALA4076)
Document: ALA3638669
BindingDB Source: 6597
Activity Charts
Bioactivity
Activity Types for Assay ALA3705519
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 460.56 | 5.15 | 5.15 |
| 473.56 | 4.47 | 4.47 |
| 460.56 | 5.15 | 5.15 |
| 443.53 | 5.33 | 5.33 |
| 446.53 | 4.67 | 4.67 |
| 457.56 | 5.89 | 5.89 |
| 477.61 | 5.74 | 5.74 |
| 474.59 | 5.53 | 5.53 |
| 457.56 | 5.64 | 5.64 |
| 473.55 | 5.34 | 5.34 |
| 458.54 | 4.91 | 4.91 |
| 476.53 | 5.05 | 5.05 |
| 475.55 | 6.03 | 6.03 |
| 460.56 | 4.97 | 4.97 |
| 443.53 | 5.33 | 5.33 |
| 457.56 | 5.26 | 5.26 |
| 501.54 | 5.11 | 5.11 |
| 461.52 | 5.47 | 5.47 |
| 457.56 | 5.26 | 5.26 |
| 491.55 | 4.61 | 4.61 |
| 458.54 | 5.03 | 5.03 |
| 501.61 | 6.29 | 6.29 |
| 474.59 | 5.53 | 5.53 |
| 433.49 | 4.92 | 4.92 |
| 512.64 | 5.93 | 5.93 |
| 526.66 | 6.27 | 6.27 |
| 501.61 | 6.29 | 6.29 |
| 528.63 | 5.16 | 5.16 |
| 486.60 | 5.39 | 5.39 |
| 511.53 | 6.35 | 6.35 |
| 474.59 | 5.83 | 5.83 |
| 444.52 | 4.72 | 4.72 |
| 472.57 | 5.34 | 5.34 |
| 475.57 | 5.58 | 5.58 |
| 483.55 | 4.97 | 4.97 |
| 443.53 | 5.33 | 5.33 |
| 443.53 | 5.33 | 5.33 |
| 489.60 | 5.97 | 5.97 |
| 477.97 | 5.98 | 5.98 |
| 477.97 | 5.98 | 5.98 |
| 482.57 | 5.58 | 5.58 |
| 459.53 | 5.03 | 5.03 |
| 457.56 | 5.26 | 5.26 |
| 461.52 | 5.47 | 5.47 |
| 475.55 | 5.39 | 5.39 |
| 475.55 | 5.39 | 5.39 |
| 479.58 | 4.88 | 4.88 |
| 433.49 | 4.92 | 4.92 |
| 451.48 | 5.06 | 5.06 |
| 482.59 | 4.21 | 4.21 |
| 478.55 | 5.11 | 5.11 |
| 503.65 | 6.83 | 6.83 |
| 505.67 | 7.07 | 7.07 |
| 463.58 | 5.95 | 5.95 |
| 477.97 | 5.98 | 5.98 |
| 476.53 | 5.17 | 5.17 |
| 434.48 | 4.31 | 4.31 |
| 457.56 | 5.64 | 5.64 |
| 449.56 | 5.39 | 5.39 |
| 460.56 | 4.97 | 4.97 |
| 460.56 | 4.97 | 4.97 |
| 457.56 | 5.64 | 5.64 |
| 461.54 | 5.54 | 5.54 |
| 460.56 | 4.97 | 4.97 |
| 474.59 | 5.28 | 5.28 |
| 477.97 | 5.98 | 5.98 |
| 447.52 | 5.23 | 5.23 |
| 460.56 | 5.15 | 5.15 |
| 460.56 | 4.97 | 4.97 |
| 460.56 | 5.15 | 5.15 |
| 463.58 | 5.70 | 5.70 |
| 460.56 | 4.82 | 4.82 |
| 473.55 | 5.34 | 5.34 |
| 475.57 | 5.47 | 5.47 |
| 461.54 | 5.54 | 5.54 |
| 443.53 | 5.33 | 5.33 |
| 477.97 | 5.98 | 5.98 |
| 461.52 | 5.47 | 5.47 |
| 457.56 | 5.64 | 5.64 |
| 528.63 | 5.16 | 5.16 |
| 458.54 | 4.91 | 4.91 |
| 446.53 | 4.67 | 4.67 |
| 444.52 | 4.72 | 4.72 |
| 459.53 | 5.03 | 5.03 |
| 444.52 | 4.72 | 4.72 |
| 460.56 | 4.90 | 4.90 |
| 472.57 | 5.34 | 5.34 |
| 446.53 | 4.51 | 4.51 |
| 460.56 | 5.15 | 5.15 |
| 489.60 | 5.86 | 5.86 |
| 478.53 | 3.17 | 3.17 |
| 501.61 | 6.11 | 6.11 |
| 457.56 | 5.64 | 5.64 |
| 408.57 | 6.23 | 6.23 |
| 436.58 | 5.61 | 5.61 |
| 394.54 | 5.75 | 5.75 |
| 435.55 | 4.28 | 4.28 |
| 378.50 | 5.25 | 5.25 |
| 447.52 | 5.23 | 5.23 |
| 533.65 | 6.37 | 6.37 |