Basic Information
ID: ALA3705520
Type: Binding
Description: Forward Mode Assay: The assay is based on the monitoring of intracellular Ca2+concentrations using the PBX Calcium Assay Kit from BD (Becton, Dickinson and Company) with calcium indicator dye 51-9000177BKa (BD, 640177), CHO cells expressing NCX1 were loaded with the dye, and after a preincubation period with the test compound, lonomycin (Calbiochem, 407950) was added. Ionomycin is an ionophor for Ca2+ions mediating an increase of intracellular Ca2+ions. Consequently, intracellular Ca2+ions are exchanged against extracellular Na+ions (Ca2+efflux, forward mode). The decrease of intracellular Ca2+ions was detected by measuring the fluorescence of the calcium indicator dye at a wavelength of 520 nm by a FLIPR device.Briefly, similarly as for the reverse mode, for the forward mode transport assay 18000 cells per well were seeded into a 96 well microplate (Corning COSTAR 3904) and incubated overnight in culture medium (cf. above). A total volume of 100 ul medium per well was used.
Format: BAO_0000219
Cell type: CHO
Target: Sodium/calcium exchanger 1(ALA4076)
Document: ALA3638669
BindingDB Source: 6597
Activity Charts
Bioactivity
Activity Types for Assay ALA3705520
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 473.56 | 4.47 | 4.47 |
| 475.55 | 6.03 | 6.03 |
| 460.56 | 4.97 | 4.97 |
| 461.52 | 5.47 | 5.47 |
| 491.55 | 4.61 | 4.61 |
| 477.97 | 5.98 | 5.98 |
| 434.48 | 4.31 | 4.31 |
| 457.56 | 5.64 | 5.64 |
| 378.50 | 5.25 | 5.25 |
| 429.55 | 5.69 | 5.69 |
| 477.97 | 5.98 | 5.98 |
| 450.56 | 4.89 | 4.89 |
| 422.51 | 4.47 | 4.47 |
| 492.00 | 6.29 | 6.29 |
| 433.53 | 3.95 | 3.95 |
| 439.49 | 3.69 | 3.69 |
| 410.50 | 4.03 | 4.03 |
| 394.50 | 5.14 | 5.14 |
| 410.50 | 4.03 | 4.03 |
| 477.59 | 4.55 | 4.55 |
| 447.52 | 5.23 | 5.23 |
| 408.52 | 5.45 | 5.45 |
| 461.54 | 5.54 | 5.54 |
| 424.52 | 4.34 | 4.34 |
| 428.49 | 4.17 | 4.17 |
| 426.51 | 5.59 | 5.59 |
| 490.47 | 4.15 | 4.15 |
| 412.49 | 5.15 | 5.15 |
| 338.43 | 4.46 | 4.46 |
| 540.65 | 5.96 | 5.96 |
| 449.60 | 6.42 | 6.42 |
| 392.48 | 4.89 | 4.89 |