Basic Information
ID: ALA3705527
Type: Binding
Description: Binding Assay: MCP-1 Receptor Binding Assay in THP-1 Cells Human monocytic cell line THP-1 cells were obtained from American Type Culture Collection (Manassas, Va., USA). The THP-1 cells were grown in RPMI-1640 (RPMI: Roswell Park Memorial Institute Medium-cell culture growth media) supplemented with 10% fetal bovine serum in a humidified 5% CO2 atmosphere at 37 C. The cell density was maintained between 0.5x106 cells/mL.THP-1 (cells were incubated with 0.5 nM 125I labeled MCP-1 (Perkin-Elmer Life Sciences, Inc. Boston, Mass.) in the presence of varying concentrations of either unlabeled MCP-1 (R & D Systems, Minneapolis, Minn.) or test compound for 2 hours at 30 C. in a 96 well plate. Cells were then harvested onto a filter plate, dried, and 20 uL of Microscint 20 was added to each well. Plates were counted in a TopCount NXT, Microplate Scintillation & Luminescence Counter (Perkin-Elmer Life Sciences, Inc. Boston, Mass.).
Format: BAO_0000219
Cell type: THP-1
Target: C-C chemokine receptor type 2(ALA4015)
Document: ALA3638678
BindingDB Source: 6619
Activity Charts
Bioactivity
Activity Types for Assay ALA3705527
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 553.63 | 5.69 | 5.69 |
| 538.61 | 5.01 | 5.01 |
| 552.64 | 5.27 | 5.27 |
| 566.67 | 5.61 | 5.61 |
| 554.61 | 5.03 | 5.03 |
| 539.60 | 4.40 | 4.40 |
| 539.60 | 4.40 | 4.40 |
| 530.59 | 3.93 | 3.93 |
| 484.64 | 4.59 | 4.59 |
| 502.63 | 4.73 | 4.73 |
| 502.63 | 4.73 | 4.73 |
| 502.63 | 4.73 | 4.73 |
| 519.09 | 5.24 | 5.24 |
| 552.64 | 5.61 | 5.61 |
| 568.64 | 5.49 | 5.49 |
| 520.62 | 4.87 | 4.87 |
| 520.62 | 4.87 | 4.87 |
| 520.62 | 4.87 | 4.87 |
| 542.51 | 5.89 | 5.89 |
| 541.62 | 5.60 | 5.60 |
| 552.64 | 5.61 | 5.61 |
| 557.62 | 5.48 | 5.48 |
| 524.71 | 5.08 | 5.08 |
| 526.68 | 4.52 | 4.52 |
| 541.62 | 5.60 | 5.60 |
| 564.63 | 4.91 | 4.91 |
| 545.68 | 4.76 | 4.76 |
| 491.61 | 4.72 | 4.72 |
| 545.68 | 4.76 | 4.76 |
| 542.68 | 4.36 | 4.36 |
| 535.64 | 5.56 | 5.56 |
| 521.61 | 5.47 | 5.47 |
| 539.60 | 5.61 | 5.61 |
| 543.50 | 6.49 | 6.49 |
| 543.50 | 6.49 | 6.49 |
| 491.61 | 4.72 | 4.72 |
| 527.59 | 5.00 | 5.00 |
| 519.72 | 5.30 | 5.30 |
| 509.60 | 4.86 | 4.86 |
| 540.71 | 4.91 | 4.91 |
| 554.74 | 5.30 | 5.30 |
| 538.61 | 5.01 | 5.01 |
| 538.61 | 5.01 | 5.01 |
| 543.50 | 5.28 | 5.28 |
| 539.60 | 4.40 | 4.40 |
| 543.50 | 5.28 | 5.28 |
| 485.63 | 3.98 | 3.98 |
| 521.61 | 4.26 | 4.26 |
| 521.61 | 4.26 | 4.26 |
| 503.62 | 4.12 | 4.12 |
| 488.64 | 4.29 | 4.29 |
| 540.63 | 4.57 | 4.57 |