Basic Information
ID: ALA3705528
Type: Binding
Description: Inhibition Assay: Enzymatic reactions were carried out in a reaction containing 50 mM NaH2PO4, 100 mM NaCl and 0.1% BSA (pH 7.0) using 2 mM 4-Methylumbelliferyl N-acetyl-β-D-glucosaminide dihydrate (Sigma M2133) dissolved in ddH2O, as a substrate. The amount of purified human O-GlcNAcase enzyme used in the reaction was 0.7 nM. Test compound of varying concentrations was added to the enzyme prior to initiation of the reaction. The reaction was performed at room temperature in a 96-well plate and was initiated with the addition of substrate. The production of fluorescent product was measured every 60 sec for 45 min with a Tecan Infinite M200 plate-reader with excitation at 355 nM and emission detected at 460 nM, with 4-Methylumbelliferone (Sigma M1381) used to produce a standard curve. The slope of product production was determined for each concentration of compound tested and plotted, using standard curve fitting algorithms for sigmoidal dose response curves.
Organism: Homo sapiens
Activity Charts
Bioactivity
Activity Types for Assay ALA3705528
Ki
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 280.32 | -1.15 | -1.15 |
| 288.37 | -0.71 | -0.71 |
| 274.34 | -1.10 | -1.10 |
| 276.36 | -0.76 | -0.76 |
| 274.34 | -0.99 | -0.99 |
| 264.30 | -1.61 | -1.61 |
| 276.31 | -1.58 | -1.58 |
| 291.33 | -1.78 | -1.78 |