Basic Information
ID: ALA3705585
Type: Binding
Description: TR-FRET Assay: For the assay 50 nl of a 100 fold concentrated solution of the test compound in DMSO was pipetted into a black low volume 384 well microtiter plate (Greiner Bio-One, Frickenhausen, Germany), 2 ul of a solution of Akt2 in assay buffer [50 mM TRIS/HCl pH 7.5, 5 mM MgCl2, 1 mM dithiothreitol, 0.02% (v/v) Triton X-100 (Sigma)] were added and the mixture was incubated for 15 min at 22 C. to allow prebinding of the test compounds to the enzyme before the start of the kinase reaction. Then the kinase reaction was started by the addition of 3 ul of a solution of adenosine-tri-phosphate (ATP, 16.7 uM=>final conc. in the 5 ul assay volume is 10 uM) and substrate (1.67 uM=>final conc. in the 5 ul assay volume is 1 uM) in assay buffer and the resulting mixture was incubated for a reaction time of 60 min at 22 C. The concentration of Akt2 in the assay was adjusted depending of the activity of the enzyme lot.
Format: BAO_0000357
Organism: Homo sapiens
Target: Serine/threonine-protein kinase AKT2(ALA2431)
Document: ALA3638633
BindingDB Source: 6718
Activity Charts
Bioactivity
Activity Types for Assay ALA3705585
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 382.47 | 4.11 | 4.11 |
| 340.43 | 4.40 | 4.40 |
| 354.46 | 4.71 | 4.71 |
| 380.50 | 5.28 | 5.28 |
| 416.53 | 6.07 | 6.07 |
| 430.56 | 6.38 | 6.38 |
| 354.46 | 4.71 | 4.71 |
| 370.46 | 4.41 | 4.41 |
| 384.48 | 4.80 | 4.80 |
| 416.53 | 6.07 | 6.07 |
| 364.45 | 4.88 | 4.88 |
| 405.51 | 5.19 | 5.19 |
| 427.50 | 4.80 | 4.80 |
| 412.49 | 4.11 | 4.11 |
| 411.51 | 4.76 | 4.76 |
| 433.52 | 4.55 | 4.55 |
| 405.51 | 5.19 | 5.19 |
| 370.46 | 4.41 | 4.41 |
| 356.43 | 4.11 | 4.11 |
| 398.51 | 5.19 | 5.19 |
| 364.45 | 4.88 | 4.88 |
| 353.47 | 5.31 | 5.31 |
| 353.47 | 5.31 | 5.31 |
| 364.45 | 4.88 | 4.88 |
| 353.47 | 5.31 | 5.31 |
| 369.47 | 5.01 | 5.01 |
| 396.49 | 4.37 | 4.37 |
| 383.50 | 5.15 | 5.15 |
| 369.47 | 5.01 | 5.01 |
| 383.50 | 5.06 | 5.06 |
| 369.47 | 4.50 | 4.50 |
| 369.47 | 4.50 | 4.50 |
| 369.47 | 5.01 | 5.01 |
| 382.47 | 4.11 | 4.11 |
| 383.50 | 5.40 | 5.40 |
| 367.50 | 5.57 | 5.57 |
| 405.51 | 5.40 | 5.40 |
| 397.48 | 4.79 | 4.79 |
| 426.56 | 4.74 | 4.74 |
| 369.47 | 4.50 | 4.50 |
| 477.57 | 5.38 | 5.38 |
| 414.51 | 4.43 | 4.43 |
| 477.57 | 5.38 | 5.38 |
| 398.51 | 5.19 | 5.19 |
| 438.58 | 6.11 | 6.11 |
| 432.53 | 6.19 | 6.19 |
| 368.48 | 4.96 | 4.96 |
| 433.53 | 6.81 | 6.81 |
| 397.48 | 4.79 | 4.79 |
| 397.48 | 4.79 | 4.79 |
| 379.51 | 5.88 | 5.88 |
| 367.50 | 5.57 | 5.57 |
| 418.34 | 5.77 | 5.77 |
| 418.34 | 5.77 | 5.77 |
| 436.56 | 5.24 | 5.24 |
| 411.51 | 5.18 | 5.18 |
| 357.43 | 5.15 | 5.15 |
| 373.89 | 5.66 | 5.66 |
| 357.43 | 5.15 | 5.15 |
| 379.51 | 5.88 | 5.88 |
| 365.48 | 5.65 | 5.65 |
| 365.48 | 5.65 | 5.65 |
| 405.51 | 5.40 | 5.40 |
| 383.45 | 4.70 | 4.70 |
| 448.36 | 5.78 | 5.78 |
| 424.55 | 5.14 | 5.14 |
| 428.51 | 4.71 | 4.71 |
| 426.52 | 3.73 | 3.73 |
| 440.55 | 4.38 | 4.38 |
| 422.53 | 4.90 | 4.90 |
| 436.56 | 5.15 | 5.15 |
| 398.47 | 4.13 | 4.13 |
| 431.92 | 5.45 | 5.45 |
| 410.52 | 4.71 | 4.71 |
| 410.52 | 4.76 | 4.76 |
| 379.51 | 5.88 | 5.88 |
| 467.57 | 4.40 | 4.40 |
| 453.55 | 4.05 | 4.05 |
| 373.89 | 5.66 | 5.66 |
| 357.43 | 5.15 | 5.15 |
| 433.53 | 6.81 | 6.81 |
| 433.53 | 6.81 | 6.81 |
| 446.55 | 6.08 | 6.08 |
| 405.51 | 5.40 | 5.40 |
| 455.57 | 6.55 | 6.55 |
| 367.50 | 5.62 | 5.62 |
| 387.91 | 5.97 | 5.97 |
| 375.42 | 5.28 | 5.28 |
| 387.91 | 5.97 | 5.97 |
| 490.40 | 5.95 | 5.95 |
| 408.33 | 6.31 | 6.31 |
| 394.48 | 4.89 | 4.89 |
| 408.33 | 6.31 | 6.31 |
| 466.81 | 6.73 | 6.73 |
| 432.37 | 6.08 | 6.08 |
| 482.00 | 7.77 | 7.77 |
| 453.98 | 6.36 | 6.36 |
| 433.56 | 6.01 | 6.01 |
| 430.56 | 6.38 | 6.38 |
| 419.53 | 5.70 | 5.70 |