Basic Information
ID: ALA3705601
Type: Binding
Description: Binding Assay: cDNA sequence encoding a mouse NPY Y5 receptor (Biochim. Biophys. Acta 1328:83-89, 1997) was cloned in a vector (pME18S, Takebe et al. Mol. Cell. Biol. 8, 8957). The obtained expression vector was transfected into CHO cells as a host by using Lipofect AMINE reagent (Trademark, Gico BRL Co., Ltd.) according to the instruction manual. The cells that stably express NPY Y5 receptor were obtained.The membranes prepared from the CHO cells expressing NPY Y5 receptor, the compound of this invention and 30,000 cpm [125I] peptide YY (60 pM of final concentration: Healthcare) were incubated in the assay buffer (20 mM HEPES-Hanks buffer containing 0.1% bovine serum albumin, pH 7.4) at 25° C. for 2 hours, and then the membrane was filtered from the mixture through a glass filter (GF/C) presoaked with 1% polyethyleneimine. After washing with 50 mM Tris-HCl buffer (pH 7.4), radioactivity retained on the filters was quantified with a gamma counter.
Organism: Mus musculus
Cell type: CHO
Activity Charts
Bioactivity
Activity Types for Assay ALA3705601
IC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 382.46 | 2.79 | 2.79 |
| 364.47 | 2.65 | 2.65 |
| 382.46 | 2.79 | 2.79 |
| 396.49 | 3.17 | 3.17 |
| 368.43 | 2.40 | 2.40 |
| 378.50 | 3.04 | 3.04 |
| 368.43 | 2.40 | 2.40 |
| 400.45 | 2.92 | 2.92 |
| 400.45 | 2.92 | 2.92 |
| 400.45 | 2.92 | 2.92 |
| 384.89 | 2.91 | 2.91 |
| 397.48 | 2.57 | 2.57 |
| 411.50 | 2.96 | 2.96 |
| 434.61 | 4.08 | 4.08 |
| 384.42 | 2.78 | 2.78 |
| 374.48 | 2.72 | 2.72 |
| 420.53 | 3.45 | 3.45 |
| 370.40 | 2.39 | 2.39 |
| 383.44 | 3.38 | 3.38 |
| 406.50 | 3.05 | 3.05 |
| 434.43 | 3.41 | 3.41 |
| 369.41 | 2.99 | 2.99 |
| 420.40 | 3.02 | 3.02 |
| 406.38 | 2.63 | 2.63 |
| 424.49 | 3.35 | 3.35 |