Basic Information
ID: ALA3705610
Type: Binding
Description: SPA Assay: The Glucagon SPA assay is used to determine the ability of test compounds to block the binding of glucagon-cex to the glucagon receptor. Test compounds are re-suspended and serially diluted in 100% DMSO. 1 ul of test compound at the desired concentrations is spotted into the appropriate wells of 96 well low binding white clear bottom plate (Corning). 1 ul of DMSO is spotted into total binding wells. 1 ul of a known glucagon antagonist at a concentration of 20 uM is added to non specific binding wells. 0.3-0.75 ug of membrane from chem-1 cells stably transfected with the human glucagon receptor (Millipore), 125 pM of [125I]Glucagon-Cex (Perkin Elmer) and 175 ug of WGA PVT SPA beads (Perkin Elmer) are added to all wells of the assay plate. All assay ingredients with the exception of test compounds are re-suspended in the following buffer; 50 mM Hepes pH 7.4; 5 mM MgCl2; 1 mM CaCl; 5% glycerol and 0.2% BSA.
Organism: Homo sapiens
Activity Charts
Bioactivity
Activity Types for Assay ALA3705610
Ki
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 445.56 | 5.34 | 5.34 |
| 463.55 | 5.48 | 5.48 |
| 405.50 | 4.70 | 4.70 |
| 405.50 | 4.70 | 4.70 |
| 405.50 | 4.64 | 4.64 |
| 424.48 | 4.17 | 4.17 |
| 405.50 | 4.64 | 4.64 |
| 435.52 | 4.65 | 4.65 |
| 420.51 | 4.34 | 4.34 |
| 405.50 | 4.64 | 4.64 |
| 419.53 | 4.95 | 4.95 |
| 419.53 | 4.95 | 4.95 |
| 437.52 | 5.09 | 5.09 |
| 439.94 | 5.29 | 5.29 |
| 473.50 | 5.66 | 5.66 |
| 473.50 | 5.66 | 5.66 |
| 419.53 | 4.95 | 4.95 |
| 419.53 | 4.95 | 4.95 |
| 463.55 | 5.48 | 5.48 |
| 449.53 | 5.17 | 5.17 |
| 449.53 | 5.17 | 5.17 |
| 419.53 | 4.95 | 4.95 |
| 431.41 | 4.54 | 4.54 |