Basic Information
ID: ALA3705626
Type: Binding
Description: ELISA Assay: HEK293 cells are seeded in 96-well poly-lysine coated plates at 20,000 cells per well in DMEM (10% FBS, 1% NEAA, 0.1% glutamine). Following overnight incubation, the cells are washed with 100 uL of Opti-MEM (Gibco, Carlsbad, Calif.) to remove serum. Compound in DMSO is serially diluted (beginning with 100 μM) in Opti-MEM and added to the cells. The conditioned media is analyzed for VEGF with a Quantikine human VEGF immunoassay kit (R&D Systems, Minneapolis, Minn.).
Organism: Homo sapiens
Cell type: HEK293
Activity Charts
Bioactivity
Activity Types for Assay ALA3705626
EC50
Compound Summary
| Parent Molecular Weight | ALogP | Polar Surface Area |
|---|---|---|
| 320.73 | 2.01 | 2.01 |
| 320.73 | 2.01 | 2.01 |
| 311.30 | 1.91 | 1.91 |
| 286.29 | 1.58 | 1.58 |
| 285.30 | 2.18 | 2.18 |
| 300.31 | 1.67 | 1.67 |
| 320.73 | 2.01 | 2.01 |
| 297.27 | 1.82 | 1.82 |
| 306.71 | 1.92 | 1.92 |
| 314.34 | 2.39 | 2.39 |
| 290.25 | 1.41 | 1.41 |
| 330.34 | 2.06 | 2.06 |
| 320.73 | 2.23 | 2.23 |
| 319.75 | 1.58 | 1.58 |
| 311.30 | 1.91 | 1.91 |
| 354.33 | 0.54 | 0.54 |
| 297.27 | 1.82 | 1.82 |
| 329.31 | 0.46 | 0.46 |
| 383.40 | 1.59 | 1.59 |
| 369.38 | 1.50 | 1.50 |
| 340.30 | 0.45 | 0.45 |