Inhibition Assay: Human recombinant adrenergic β2 receptors expressed in CHO cells are used in modified Tris-HCl buffer pH 7.4. A 50 aliquot is incuba...

ID: ALA3705663

Type: Binding

Description: Inhibition Assay: Human recombinant adrenergic β2 receptors expressed in CHO cells are used in modified Tris-HCl buffer pH 7.4. A 50 aliquot is incubated with 0.2 nM [3H]CGP-12177 for 60 minutes at 25° C. Non-specific binding is estimated in the presence of 10 μM ICI-118551. Receptors are filtered and washed, the filters are then counted to determine [3H]CGP-12177 specifically bound. Compounds are screened at 10 μM.

Format: BAO_0000219

Organism: Homo sapiens

Cell type: CHO

Target:  Beta-2 adrenergic receptor(ALA210)

Document:  ALA3639212

BindingDB Source:  7107