Assay Report Card

ID: ALA3705730

Type: Binding

Description: Binding Assay: Streptavidin (20 ul) (10 ug/ml Streptavidin type II (Wako Pure Chemical Industries, Ltd.), 10 mM Tris-HCl (pH 7.5), 10 mM NaCl) was added to a 384 well black plate (Nunc MaxiSorp, Thermo Fisher Scientific Inc.), and the plate was subjected to centrifugation (1000 rpm, 1 min) and coated overnight at 4 C. The plate was washed twice with PBST (PBS, 0.05% Tween 20, 100 ul/well) and blocked with 25% Block Ace (Snow Brand Milk Products Co., Ltd., PBS, 100 ul/well). The plate was subjected to centrifugation (1000 rpm, 1 min) and incubated at room temperature for 4 hr or overnight at 4 C. The plate was washed twice with PBST (PBS, 0.05% Tween 20, 100 ul/well), and biotinylate human TTR (stock solution concentration 1.0 mg/ml) diluted 750-fold with PBST was added at 20 ul/well. The plate was subjected to centrifugation (1000 rpm, 1 min) and stood still at room temperature for 1.5 hr or overnight at 4 C. The plate was washed 3 times with PBST (100 ul/well).

Organism: Homo sapiens